"St\u00E1dn\u00EDk, Lud\u011Bk" . . "\u0160palekov\u00E1, Eli\u0161ka" . "RIV/49608851:_____/13:#0000593!RIV14-MZE-49608851" . . "RIV/49608851:_____/13:#0000593" . . "Breed differences in several characteristics of bull frozen-thawed sperm"@en . . "This study was aimed at comparison of some sperm characteristics in Czech Fleckvieh (n= 8) and Holstein (n= 8) bulls at the age of 1 to 6 years. After determination of pathologic sperm (staining with Congo red, Bromothymol blue and Janus green) and sperm motility, the samples of semen with progressive movement >=70% were diluted in AndroMed extender containing soybean lecithin extract, frozen in a programmable freezing device and stored in liquid nitrogen until analyses. Sperm motility was analyzed by CASA. Sperm plasma membrane integrity and apoptosis incidence were detected under a Leica fluorescent microscope after staining with PNA-Alexa Fluor and Yo-Pro-I in combination with propidium iodide (PI), using specific wavelength filters. Average age of bulls and incidence of pathological sperm were not different between CF and H breeds, whilst average body condition score (BCS) was significantly higher (p< 0,05) in CF (2.84\u00B10, 17) than in H (2.19\u00B10, 13) bulls. Total sperm motility and progressive movement measured immediately after thawing (38.6\u00B11.8 and 34.19\u00B11.9, resp) and following 0,5 h (46.44\u00B11.79 and 44,14\u00B11.8) were higher in H bulls compared to CF bulls (34,15\u00B12 and 30.27\u00B12,13; 39,17 \u00B12 and 36.12\u00B12.07, resp.), but following 2 h these traits were not different. More sperm with plasma membrane damages were recorded in CF bulls (36.41\u00B11.81%) compared to H bulls (26.33\u00B1 1.63%). Similarly, higher incidence of apoptotic sperm was noted in CF bulls (19.12\u00B11.71%) compared to H bulls (12.58\u00B1 1.51 %). We observed differences in sperm motility and viability between two tested breeds, which may be a result of interbreed difference in average body condition. Makarevi\u010D., A. \u2013 \u0160palekov\u00E1, E. \u2013 St\u00E1dn\u00EDk, L. \u2013 Kubovi\u010Dov\u00E1, E. \u2013 Louda, F.: Breed differences in several characteristics of bull frozen-thawed sperm. 10th Int. Sci. Conference Animal Physiology 2013 (June 4th-6th 2013; Crocus-Ke\u017Emarsk\u00E9 \u017D\u013Eaby). In: J. Microbiol. Biotechnol. Food Sci., 2013:2 (Special Issue), s. 6." . . . "P(QI91A061)" . "Kubovi\u010Dov\u00E1, Elena" . . . "Holstein; Czech Fleckvieh; bull; sperm; motility; plasma membrane; apoptosis"@en . "Breed differences in several characteristics of bull frozen-thawed sperm" . "1"^^ . . "[ADDC9E2B4FF4]" . . . . . "Louda, Franti\u0161ek" . "Makarevi\u010D, Alexander" . "Breed differences in several characteristics of bull frozen-thawed sperm"@en . . "5"^^ . . . "Breed differences in several characteristics of bull frozen-thawed sperm" . . . . "This study was aimed at comparison of some sperm characteristics in Czech Fleckvieh (n= 8) and Holstein (n= 8) bulls at the age of 1 to 6 years. After determination of pathologic sperm (staining with Congo red, Bromothymol blue and Janus green) and sperm motility, the samples of semen with progressive movement >=70% were diluted in AndroMed extender containing soybean lecithin extract, frozen in a programmable freezing device and stored in liquid nitrogen until analyses. Sperm motility was analyzed by CASA. Sperm plasma membrane integrity and apoptosis incidence were detected under a Leica fluorescent microscope after staining with PNA-Alexa Fluor and Yo-Pro-I in combination with propidium iodide (PI), using specific wavelength filters. Average age of bulls and incidence of pathological sperm were not different between CF and H breeds, whilst average body condition score (BCS) was significantly higher (p< 0,05) in CF (2.84\u00B10, 17) than in H (2.19\u00B10, 13) bulls. Total sperm motility and progressive movement measured immediately after thawing (38.6\u00B11.8 and 34.19\u00B11.9, resp) and following 0,5 h (46.44\u00B11.79 and 44,14\u00B11.8) were higher in H bulls compared to CF bulls (34,15\u00B12 and 30.27\u00B12,13; 39,17 \u00B12 and 36.12\u00B12.07, resp.), but following 2 h these traits were not different. More sperm with plasma membrane damages were recorded in CF bulls (36.41\u00B11.81%) compared to H bulls (26.33\u00B1 1.63%). Similarly, higher incidence of apoptotic sperm was noted in CF bulls (19.12\u00B11.71%) compared to H bulls (12.58\u00B1 1.51 %). We observed differences in sperm motility and viability between two tested breeds, which may be a result of interbreed difference in average body condition. Makarevi\u010D., A. \u2013 \u0160palekov\u00E1, E. \u2013 St\u00E1dn\u00EDk, L. \u2013 Kubovi\u010Dov\u00E1, E. \u2013 Louda, F.: Breed differences in several characteristics of bull frozen-thawed sperm. 10th Int. Sci. Conference Animal Physiology 2013 (June 4th-6th 2013; Crocus-Ke\u017Emarsk\u00E9 \u017D\u013Eaby). In: J. Microbiol. Biotechnol. Food Sci., 2013:2 (Special Issue), s. 6."@en . "63852" . .