. "9"^^ . "Effect of insulin-like growth factor I on functional parameters of ram cooled-stored spermatozoa"@en . . "\u0160palekov\u00E1, Eli\u0161ka" . . "P(QH81324)" . . "Makarevi\u010D, Alexander" . "The study was aimed at examining the effects of IGF-I on ram sperm traits after hypothermic (4\u00BAC) storage. Sperm ejaculates from 3 Lacaune rams were diluted in Triladyl, pooled and divided into the groups of IGF-I doses added (0, 10, 100 or 200 ng/ml). Following 72 h of storage the sperm samples were stained for a plasma membrane integrity (peanut agglutinin, PNA-FITC), membrane stability (annexin V-FITC) and apoptosis (Yo-Pro-1) and analyzed under a fluorescent microscope. Sperm motility was determined by CASA (at 0, 24 and 48h) and fertilizing ability (at 48h) - by an in vitro fertilization (IVF) test on bovine oocytes. IGF-I (100 and 200 ng/ml) decreased the portion of spermatozoa with disrupted plasma membrane integrity (15.9 and 16.5% resp. vs 28.3% in control, p< 0.05) and, given at all tested doses, reduced the rate of apoptotic sperm (6.2, 4.9 and 4.4% resp. vs 9.7% in control, p< 0.05). The proportion of spermatozoa with annexin V-labelled membrane changes was reduced by IGF-I from 9.6% (control) to 4.9% (100 ng/ml) and 6.1% (200 ng/ml). IGF-I (10 ng/ml), elevated sperm motility measured after 24 h storage (83.3% vs. 69.2% in control); the higher doses had no effect. IGF-I, given at 100 ng/ml, increased sperm fertilization rate (67.8% vs. 47.0%, p<0.05). In summary, IGF-I improved ram sperm functions during cooling storage and their effects were reflected in fertilizing ability in vitro." . "133097" . "Kubovi\u010Dov\u00E1, Elena" . "The study was aimed at examining the effects of IGF-I on ram sperm traits after hypothermic (4\u00BAC) storage. Sperm ejaculates from 3 Lacaune rams were diluted in Triladyl, pooled and divided into the groups of IGF-I doses added (0, 10, 100 or 200 ng/ml). Following 72 h of storage the sperm samples were stained for a plasma membrane integrity (peanut agglutinin, PNA-FITC), membrane stability (annexin V-FITC) and apoptosis (Yo-Pro-1) and analyzed under a fluorescent microscope. Sperm motility was determined by CASA (at 0, 24 and 48h) and fertilizing ability (at 48h) - by an in vitro fertilization (IVF) test on bovine oocytes. IGF-I (100 and 200 ng/ml) decreased the portion of spermatozoa with disrupted plasma membrane integrity (15.9 and 16.5% resp. vs 28.3% in control, p< 0.05) and, given at all tested doses, reduced the rate of apoptotic sperm (6.2, 4.9 and 4.4% resp. vs 9.7% in control, p< 0.05). The proportion of spermatozoa with annexin V-labelled membrane changes was reduced by IGF-I from 9.6% (control) to 4.9% (100 ng/ml) and 6.1% (200 ng/ml). IGF-I (10 ng/ml), elevated sperm motility measured after 24 h storage (83.3% vs. 69.2% in control); the higher doses had no effect. IGF-I, given at 100 ng/ml, increased sperm fertilization rate (67.8% vs. 47.0%, p<0.05). In summary, IGF-I improved ram sperm functions during cooling storage and their effects were reflected in fertilizing ability in vitro."@en . "\u0159\u00EDjen 2012" . "5"^^ . . . "ram; sperm; IGF-I; viability; motility; fertilizing ability"@en . . "http://dx.doi.org/10.1017/S0967199412000500" . . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . . "Effect of insulin-like growth factor I on functional parameters of ram cooled-stored spermatozoa"@en . "Effect of insulin-like growth factor I on functional parameters of ram cooled-stored spermatozoa" . "Heged\u00FC\u0161ov\u00E1, Zde\u0148ka" . . "10.1017/S0967199412000500" . . . "RIV/49608851:_____/12:#0000581" . . "[71E140F00A66]" . "0967-1994" . . . "Olexikov\u00E1, Lucia" . "Zygote" . "Effect of insulin-like growth factor I on functional parameters of ram cooled-stored spermatozoa" . . "RIV/49608851:_____/12:#0000581!RIV13-MZE-49608851" . . . "1"^^ . .