. "Rep-PCR-based typing as a tool for tracking of MRSA infection origin" . . . . "RIV/49608851:_____/12:#0000559" . "2"^^ . "Rep-PCR-based typing as a tool for tracking of MRSA infection origin"@en . . "RIV/49608851:_____/12:#0000559!RIV13-MZE-49608851" . . . . . . "A performance of rep-PCR fi ngerprinting method with the (GTG)5 primer was explored in order to evaluate its practical application for confi rmation the source of human staphylococci infection. A laboratory worker daily working with the MRSA and Staphylococcus aureus strains has been tested positively for MRSA nosocomial infection. The collection of nine MRSA strains held in the laboratory has been typed with the rep-PCR method. Analysis of the fi ngerprints with the unweighted pairgroup method using arithmetic averages (UPGMA) clustering method revealed presence of six strain clusters with similarity lower than 85%. The fi ngerprint of MRSA strain infecting the human diff ered signifi cantly from remaining fi ngerprints. This provided clear evidence that MRSA strain infecting the personal did not come from the laboratory strains collection. Our experimental results showed rep-PCR with the (GTG)5 primer as an eff ective tool applicable for diff erentiation of individual S. aureus strains. However, the reproducibility and discrimination power of the method depended on strict observance of the optimal PCR time and temperature profi le and PCR composition as well."@en . "2"^^ . "A performance of rep-PCR fi ngerprinting method with the (GTG)5 primer was explored in order to evaluate its practical application for confi rmation the source of human staphylococci infection. A laboratory worker daily working with the MRSA and Staphylococcus aureus strains has been tested positively for MRSA nosocomial infection. The collection of nine MRSA strains held in the laboratory has been typed with the rep-PCR method. Analysis of the fi ngerprints with the unweighted pairgroup method using arithmetic averages (UPGMA) clustering method revealed presence of six strain clusters with similarity lower than 85%. The fi ngerprint of MRSA strain infecting the human diff ered signifi cantly from remaining fi ngerprints. This provided clear evidence that MRSA strain infecting the personal did not come from the laboratory strains collection. Our experimental results showed rep-PCR with the (GTG)5 primer as an eff ective tool applicable for diff erentiation of individual S. aureus strains. However, the reproducibility and discrimination power of the method depended on strict observance of the optimal PCR time and temperature profi le and PCR composition as well." . "6" . "I, P(LA10029), P(QH81111)" . . "165055" . "Vylet\u011Blov\u00E1, Marcela" . . . . . "[99D271F660DE]" . . "Acta Universitatis Agriculturae et Silviculturae Mendeleianae Brunensis" . "6"^^ . . . "1211-8516" . . "Rep-PCR-based typing as a tool for tracking of MRSA infection origin" . "rep-PCR; fingerprinting; MRSA; infection"@en . . "CZ - \u010Cesk\u00E1 republika" . "Rep-PCR-based typing as a tool for tracking of MRSA infection origin"@en . "60" . "Manga, Ivan" .