"The selected set of twenty inter-line F1 hybrids of Brassica napus L. was evaluated for their capacity to produce microspore-derived doubled haploid (DH) plants subsequently utilized in the breeding \u201ECzech Winter Rape''. All tested genotypes were able to produce microspore-derived embryos based on the standardized protocol previously adopted for the Czech winter rape varieties/breeding lines; nevertheless, significant differences were found in the frequency of microspore-derived embryos production as related to particular genotypes. However, the fertile dihaploid DH regenerants were obtained only in 12 hybrid lines of this set. The other 8 lines failed to produce fertile DH plants for several reasons, mainly due to altered morphology of microspore embryos connected with limited germination/conversion potential and due to unsuccessful colchicine treatment resulting in haploid or mixoploid (chimeric) regenerants exhibiting partial or full sterility. In general, colchicine treatment induced diploid" . "14"^^ . . "V\u011Btrovcov\u00E1, Martina" . "Efficiency of Microspore Culture for Doubled Haploid Production in the Breeding Project ''Czech Winter Rape\u201D. Czech J. Genet. Plant Breed., 42, 58-71, ISSN 1212-1975."@en . . . . "Mach\u00E1\u010Dkov\u00E1, Ivana" . "Griga, Miroslav" . "Efficiency of Microspore Culture for Doubled Haploid Production in the Breeding Project ''Czech Winter Rape\u201D. Czech J. Genet. Plant Breed., 42, 58-71, ISSN 1212-1975." . . "RIV/47116099:_____/06:R0090916" . "1212-1975" . "[8AAE366B23BE]" . . "Vybran\u00FD soubor 20 meziliniov\u00FDch F1 hybrid\u016F Brassica napus L. bylo hodnoceno z hlediska jejich schopnosti produkovat dihaploidn\u00ED (DH) rostliny odvozen\u00E9 z mikrosp\u00F3rov\u00E9 kultury n\u00E1sledn\u011B vyu\u017Eit\u00FDch ve \u0161lechtitelsk\u00E9m projektu \u010Cesk\u00E1 \u0158epka. V\u0161echny testovan\u00E9 genotypy byly schopny regenerovat embrya z mikrosp\u00F3r podle standardizovan\u00E9ho protokolu, kter\u00FD byl ji\u017E d\u0159\u00EDve upraven pro odr\u016Fdy/\u0161lechtitelsk\u00E9 linie \u010Cesk\u00E9 \u0158epky; byly zji\u0161t\u011Bny v\u00FDznamn\u00E9 rozd\u00EDly v tvorb\u011B embry\u00ED mezi jednotliv\u00FDmi genotypy. Fertiln\u00ED DH regeneranti byly z\u00EDsk\u00E1ni z 12 lini\u00ED souboru. Ostan\u00EDch 8 lini\u00ED v podstat\u011B netvo\u0159ilo DH rostliny z r\u016Fzn\u00FDch d\u016Fvod\u016F, zejm\u00E9na v d\u016Fsledku zm\u011Bn\u011Bn\u00E9 morfologie mikrospor\u00F3rov\u00FDch embry\u00ED ve spojen\u00ED s omezenou schopnost\u00ED kl\u00ED\u010Den\u00ED/konverze a v d\u016Fsledku ne\u00FAsp\u011B\u0161n\u00E9ho o\u0161et\u0159en\u00ED kolchicinem, kter\u00E9 m\u011Blo za n\u00E1sledek haploidn\u00ED nebo mixoploidn\u00ED (chimerick\u00E9) regeneranty vykazuj\u00EDc\u00ED \u010D\u00E1ste\u010Dnou nebo \u00FAplnou sterilitu. Obecn\u011B lze konstatovat, \u017Ee kolchicinov\u00E9 o\u0161et\u0159en\u00ED idukovalo diploidn\u00ED, polyploidn\u00ED, (triploidn\u00ED, tetraploidn\u00ED) a mixoploi"@cs . . . "Efficiency of Microspore Culture for Doubled Haploid Production in the Breeding Project ''Czech Winter Rape\u201D. Czech J. Genet. Plant Breed., 42, 58-71, ISSN 1212-1975." . "\u00DA\u010Dinnost mikrosporov\u00E9 kultury na tvorbu dihaploid\u016F ve \u0161lechtitelsk\u00E9m projektu ''\u010Cesk\u00E1 \u0159epka\u201D. Czech J. Genet. Plant Breed., 42, 58-71, ISSN 1212-1975."@cs . . "\u00DA\u010Dinnost mikrosporov\u00E9 kultury na tvorbu dihaploid\u016F ve \u0161lechtitelsk\u00E9m projektu ''\u010Cesk\u00E1 \u0159epka\u201D. Czech J. Genet. Plant Breed., 42, 58-71, ISSN 1212-1975."@cs . "2" . . "P(1G46061), P(QE0052)" . "The selected set of twenty inter-line F1 hybrids of Brassica napus L. was evaluated for their capacity to produce microspore-derived doubled haploid (DH) plants subsequently utilized in the breeding \u201ECzech Winter Rape''. All tested genotypes were able to produce microspore-derived embryos based on the standardized protocol previously adopted for the Czech winter rape varieties/breeding lines; nevertheless, significant differences were found in the frequency of microspore-derived embryos production as related to particular genotypes. However, the fertile dihaploid DH regenerants were obtained only in 12 hybrid lines of this set. The other 8 lines failed to produce fertile DH plants for several reasons, mainly due to altered morphology of microspore embryos connected with limited germination/conversion potential and due to unsuccessful colchicine treatment resulting in haploid or mixoploid (chimeric) regenerants exhibiting partial or full sterility. In general, colchicine treatment induced diploid"@en . "Efficiency of Microspore Culture for Doubled Haploid Production in the Breeding Project ''Czech Winter Rape\u201D. Czech J. Genet. Plant Breed., 42, 58-71, ISSN 1212-1975."@en . "microspore culture, doubled haploids, winter rape, Brassica napus L., hybrid breeding"@en . "5"^^ . "2" . . "Czech Journal of Genetics and Plant Breeding" . "Kl\u00EDma, Miroslav" . . . "RIV/47116099:_____/06:R0090916!RIV07-MZE-47116099" . . "1"^^ . . . . "Sm\u00FDkalov\u00E1, Iva" . "CZ - \u010Cesk\u00E1 republika" . "473414" . .