"197-12/K\u00DA/UKZUZ/2011" . "Molekul\u00E1rn\u00ED detekce genu rezistence hrachu k viru mozaiky p\u0159enosn\u00E9 semenem (PSbMV)"@cs . "Molecular detection of rezistance gene in pea for PSbMV virus."@en . . "2011-10-14+02:00"^^ . "Sm\u00FDkal, Petr" . . . . . . "Molekul\u00E1rn\u00ED detekce genu rezistence hrachu k viru mozaiky p\u0159enosn\u00E9 semenem (PSbMV)" . "RIV/26784246:_____/11:#0000446!RIV12-MZE-26784246" . . . "P(QI91A229)" . "Molekul\u00E1rn\u00ED detekce genu rezistence hrachu k viru mozaiky p\u0159enosn\u00E9 semenem (PSbMV)"@cs . "Virus mozaiky hrachu p\u0159enosn\u00FD semenem (Pea seed-borne mosaic virus, PSbMV) pat\u0159\u00ED v sou\u010Dasn\u00E9 dob\u011B mezi nej\u010Dast\u011Bji se vyskytuj\u00EDc\u00ED virov\u00E9 patogeny, zp\u016Fsobuj\u00EDc\u00ED v\u00E1\u017En\u00E9 \u0161kody na porostech hrachu set\u00E9ho a n\u011Bkter\u00FDch dal\u0161\u00EDch z\u00E1stupc\u016F luskovin. T\u011Bmto \u0161kod\u00E1m lze p\u0159edej\u00EDt p\u011Bs-tov\u00E1n\u00EDm rezistentn\u00EDch odr\u016Fd. Spolehliv\u00E9 markery rezistence v\u0161ak dosud chyb\u011Bly a \u0161lecht\u011Bn\u00ED bylo n\u00E1ro\u010Dn\u00E9. Byly identifikov\u00E1ny geny eIF4E resp. eIF4E(iso) odpov\u00EDdaj\u00EDc\u00ED lokus\u016Fm sbm-1 (LG VI) a sbm-2 (LG II), zprost\u0159edkuj\u00EDc\u00ED recesivn\u011B zalo\u017Eenou rezistenci k n\u011Bkolika virov\u00FDm patogen\u016Fm rodu Potyvirus, v\u010Detn\u011B PSbMV. Ur\u010Dili jsme alely eIF4E genu u vybran\u00FDch genotyp\u016F hrachu, zahrnuj\u00EDc\u00EDch v\u0161echny dostupn\u00E9 a v praxi pou\u017E\u00EDvan\u00E9 donory rezistence k PSbMV. Na z\u00E1klad\u011B sekven\u010Dn\u00ED anal\u00FDzy byly navr\u017Eeny, testov\u00E1ny a vybr\u00E1ny PCR molekul\u00E1rn\u00ED markery, umo\u017E\u0148uj\u00EDc\u00ED identifikaci jak homozygotn\u00EDch tak heterozygotn\u00EDch rostlin se 100% spolehlivost\u00ED. Touto nedestruktivn\u00ED anal\u00FDzou je mo\u017En\u00E9 uskute\u010Dnit v\u00FDb\u011Br vhodn\u00FDch jedinc\u016F v \u0159\u00E1du n\u011Bkolika hodin - na rozd\u00EDl od n\u011Bkolikadenn\u00EDch virologick\u00FDch a imunologick\u00FDch test\u016F."@cs . "Molecular detection of rezistance gene in pea for PSbMV virus."@en . "Molekul\u00E1rn\u00ED detekce genu rezistence hrachu k viru mozaiky p\u0159enosn\u00E9 semenem (PSbMV)" . . "sbm-1 detekce" . . "Virus mozaiky hrachu p\u0159enosn\u00FD semenem (Pea seed-borne mosaic virus, PSbMV) pat\u0159\u00ED v sou\u010Dasn\u00E9 dob\u011B mezi nej\u010Dast\u011Bji se vyskytuj\u00EDc\u00ED virov\u00E9 patogeny, zp\u016Fsobuj\u00EDc\u00ED v\u00E1\u017En\u00E9 \u0161kody na porostech hrachu set\u00E9ho a n\u011Bkter\u00FDch dal\u0161\u00EDch z\u00E1stupc\u016F luskovin. T\u011Bmto \u0161kod\u00E1m lze p\u0159edej\u00EDt p\u011Bs-tov\u00E1n\u00EDm rezistentn\u00EDch odr\u016Fd. Spolehliv\u00E9 markery rezistence v\u0161ak dosud chyb\u011Bly a \u0161lecht\u011Bn\u00ED bylo n\u00E1ro\u010Dn\u00E9. Byly identifikov\u00E1ny geny eIF4E resp. eIF4E(iso) odpov\u00EDdaj\u00EDc\u00ED lokus\u016Fm sbm-1 (LG VI) a sbm-2 (LG II), zprost\u0159edkuj\u00EDc\u00ED recesivn\u011B zalo\u017Eenou rezistenci k n\u011Bkolika virov\u00FDm patogen\u016Fm rodu Potyvirus, v\u010Detn\u011B PSbMV. Ur\u010Dili jsme alely eIF4E genu u vybran\u00FDch genotyp\u016F hrachu, zahrnuj\u00EDc\u00EDch v\u0161echny dostupn\u00E9 a v praxi pou\u017E\u00EDvan\u00E9 donory rezistence k PSbMV. Na z\u00E1klad\u011B sekven\u010Dn\u00ED anal\u00FDzy byly navr\u017Eeny, testov\u00E1ny a vybr\u00E1ny PCR molekul\u00E1rn\u00ED markery, umo\u017E\u0148uj\u00EDc\u00ED identifikaci jak homozygotn\u00EDch tak heterozygotn\u00EDch rostlin se 100% spolehlivost\u00ED. Touto nedestruktivn\u00ED anal\u00FDzou je mo\u017En\u00E9 uskute\u010Dnit v\u00FDb\u011Br vhodn\u00FDch jedinc\u016F v \u0159\u00E1du n\u011Bkolika hodin - na rozd\u00EDl od n\u011Bkolikadenn\u00EDch virologick\u00FDch a imunologick\u00FDch test\u016F." . "Agritec Plant Research s. r. o., ISBN 978-80-87360-08-8 \u2013 odesl\u00E1n povinn\u00FD v\u00FDtisk" . . "\u0160kody zp\u016Fsoben\u00E9 vir\u00F3zami na kvalit\u011B semen hrachu mohou dosahovat v\u00FD\u0161e a\u017E 80 % a to jak v realiza\u010Dn\u00EDch cen\u00E1ch, tak ve v\u00FDsledn\u00E9 produkci semen, p\u0159\u00EDp. osiv. Finan\u010Dn\u00ED vyj\u00E1d\u0159en\u00ED t\u00E9to skute\u010Dnosti p\u0159i pr\u016Fm\u011Brn\u00E9m v\u00FDnosu hrachu 2,4 t/ha dosahovan\u00E9m v \u010Cesk\u00E9 republice a cen\u011B poln\u00EDho hrachu (5500 K\u010D/ tunu) odpov\u00EDd\u00E1 \u010D\u00E1stce 10 560 K\u010D na hektar. Na z\u00E1klad\u011B informac\u00ED \u010CS\u00DA uv\u00E1d\u011Bn\u00FDch v Situa\u010Dn\u00ED a v\u00FDhledov\u00E9 zpr\u00E1v\u011B pro luskoviny (2011) se pr\u016Fm\u011Brn\u00E1 cena poln\u00EDho hrachu pohybuje v rozmez\u00ED 5000 \u2013 6000 korun, cena d\u0159e\u0148ov\u00E9ho hrachu je p\u0159ibli\u017En\u011B 28 000 K\u010D, by p\u0159edstavovalo zaveden\u00ED rezistentn\u00EDch genotyp\u016F \u00FAsporu v \u0159\u00E1du 117 mil. K\u010D ro\u010Dn\u011B." . "RIV/26784246:_____/11:#0000446" . . "[18D8744E603A]" . "1"^^ . . . . "Pea seed-borne mosaic virus (PSbMV) belongs among the most frequent viral pathogens causing severe losses in field pea and other legumes. These losses might be prevented by growth of resistant varieties. Reliable markers of viral resistances were not available until now. Recently eIF4E resp. eIF4E(iso) genes were identified, corresponding to sbm-1 (LG VI) and sbm-2 (LG II) loci, governing recessive resistance to potyviruses, including PSbMV. We identified alelles of eIF4E gene in selected pea genotypes, including all used resistance donors. Based on sequences we have selected and verified PCR-based markers, allowing 100% precise identification, including distinction of heterozygotes. Thus this method in contrast to tedious virological and imunological tests offers faster, cheaper and precise results directly usable in pea breeding."@en . "pea; pea seedborne mosaic virus; resistance; eIF4E; marker assisted selection; breeding"@en . . "1"^^ . . . . "213520" . "\u00DAst\u0159edn\u00ED kontroln\u00ED a zku\u0161ebn\u00ED \u00FAstav zem\u011Bd\u011Blsk\u00FD, Odd\u011Blen\u00ED fytopatologie, Hroznov\u00E1 2, 656 06 Brno" .