. "Mikrosatelity nebo jednoduch\u00E9 sekven\u010Dn\u00ED opakov\u00E1n\u00ED (SSR) jsou velmi roz\u0161\u00ED\u0159en\u00E9 kategorie repetitivn\u00EDch sekvenc\u00ED DNA, kter\u00E9 se pou\u017E\u00EDvaj\u00ED v popula\u010Dn\u00ED genetice a studi\u00EDch mapov\u00E1n\u00ED genetick\u00E9 rozmanitosti. Genetick\u00E9 a evolu\u010Dn\u00ED mechanismy z pohledu SSR nejsou zcela objasn\u011Bny. T\u0159i mikrosatelitn\u00ED lokusy s r\u016Fznou pozic\u00ED v genomu hrachu (Pisum sativum L.), A9 aktivn\u00ED lokus v LTR oblasti bohat\u00E9 retrotransposony, AD270 a AF016458 se nach\u00E1zej\u00EDc\u00ED se v oblasti genu 5'. Srovn\u00E1vac\u00ED anal\u00FDza 35 p\u00E1r\u016F vzork\u016F od sedmi odr\u016Fd vyp\u011Bstovan\u00FDch z jednoho semene po deset generac\u00ED, odhalila jednu 4 bp mutaci v 10. generaci vzorku v AD270 lokusu odpov\u00EDdaj\u00EDc\u00ED skokov\u00E9mu n\u00E1r\u016Fstu v jedn\u00E9 dal\u0161\u00ED ATCT jednotce opakov\u00E1n\u00ED.Odhadovan\u00E1 m\u00EDra mutace byla 4,76 x 10 (-3) na lokus a na generaci, s 95% intervalem spolehlivosti 1,2 x 10 (-4) a\u017E 2,7 x 10 (-2). Srovn\u00E1vac\u00ED polo\u017Eky odr\u016Fdy Bohat\u00FDr z\u00EDskan\u00E9 z r\u016Fzn\u00FDch kolekc\u00ED genov\u00FDch zdroj\u016F , uk\u00E1zaly intra a inter-odr\u016Fdovou variabilitu v doprovodn\u00FDch a opakovan\u00FDch sekvenc\u00EDch. Rozd\u00EDly ve velikosti fragmentu a st\u0159\u00EDd\u00E1n\u00ED sekvenc\u00ED byly nalezeny i v dlouhodob\u00E9m horizontu v in vitro organogenn\u00ED kultu\u0159e, zalo\u017Een\u00E9 v roce 1983 sv\u011Bd\u010D\u00EDc\u00ED o somatick\u00E9m muta\u010Dn\u00EDm procesu. D\u016Fkaz homoplasie byl zji\u0161t\u011Bn v r\u00E1mci nez\u00E1visl\u00FDch genotyp\u016F hrachu, kter\u00E1 nep\u0159\u00EDzniv\u011B ovliv\u0148uje spolehlivost genov\u00FDch zdroj\u016F, ale tak\u00E9 vysoce pro\u0161lecht\u011Bn\u00E9ho materi\u00E1l\u016F. Z\u00E1v\u011Bry t\u00E9to studie maj\u00ED d\u016Fle\u017Eit\u00FD v\u00FDznam pro studium fylogeneze rodu Pisum, ur\u010Den\u00ED odr\u016Fdy a postup registrace odr\u016Fd hrachu, kde m\u00EDra mutace ovliv\u0148uje genetickou rozmanitost a efektivn\u00EDch odhady velikosti v\u00FDchoz\u00ED populace."@cs . "11"^^ . . . . . . "4" . "Odhad mikrosatelitn\u00ED mutace hrachu (Pisum sativum L.) stanoven\u00FD na z\u00E1klad\u011B anal\u00FDz rodokmenu a anal\u00FDzy p\u016Fvodu jednoho semene"@cs . "Sm\u00FDkal, Petr" . "Homoplasy; Microsatellites; Mutation rate; Pea; Pedigree; Pisum sativum; Simple sequence repeats"@en . . . . . "Odhad mikrosatelitn\u00ED mutace hrachu (Pisum sativum L.) stanoven\u00FD na z\u00E1klad\u011B anal\u00FDz rodokmenu a anal\u00FDzy p\u016Fvodu jednoho semene" . "10.1007/s13353-011-0058-9" . "217626" . . "Odhad mikrosatelitn\u00ED mutace hrachu (Pisum sativum L.) stanoven\u00FD na z\u00E1klad\u011B anal\u00FDz rodokmenu a anal\u00FDzy p\u016Fvodu jednoho semene"@cs . "RIV/26784246:_____/11:#0000425" . "Estimation of pea (Pisum sativum L.) microsatellite mutation rate based on pedigree and single-seed descent analyses"@en . "JOURNAL OF APPLIED GENETICS" . "RIV/26784246:_____/11:#0000425!RIV12-MZE-26784246" . "1234-1983" . "Estimation of pea (Pisum sativum L.) microsatellite mutation rate based on pedigree and single-seed descent analyses"@en . "Odhad mikrosatelitn\u00ED mutace hrachu (Pisum sativum L.) stanoven\u00FD na z\u00E1klad\u011B anal\u00FDz rodokmenu a anal\u00FDzy p\u016Fvodu jednoho semene" . "Fialov\u00E1, Eva" . "52" . "http://www.springerlink.com/content/h07640m38373872u/" . . "I, S, Z(MSM2678424601)" . "DE - Spolkov\u00E1 republika N\u011Bmecko" . . "3"^^ . "Microsatellites, or simple sequence repeats (SSRs) are widespread class of repetitive DNA sequences, used in population genetics, genetic diversity and mapping studies. In spite of the SSR utility, the genetic and evolutionary mechanisms are not fully understood. We have investigated three microsatellite loci with different position in the pea (Pisum sativum L.) genome, the A9 locus residing in LTR region of abundant retrotransposon, AD270 as intergenic and AF016458 located in 5'untranslated region of expressed gene. Comparative analysis of a 35 pair samples from seven pea varieties propagated by single-seed descent for ten generations, revealed single 4 bp mutation in 10th generation sample at AD270 locus corresponding to stepwise increase in one additional ATCT repeat unit. The estimated mutation rate was 4.76 x 10(-3) per locus per generation, with a 95% confidence interval of 1.2 x 10(-4) to 2.7 x 10(-2). The comparison of cv. BohatA1/2r accessions retrieved from different collections, showed intra-, inter-accession variation and differences in flanking and repeat sequences. Fragment size and sequence alternations were also found in long term in vitro organogenic culture, established at 1983, indicative of somatic mutation process. The evidence of homoplasy was detected across of unrelated pea genotypes, which adversely affects the reliability of diversity estimates not only for diverse germplasm but also highly bred material. The findings of this study have important implications for Pisum phylogeny studies, variety identification and registration process in pea breeding where mutation rate influences the genetic diversity and the effective population size estimates."@en . . . "[44FAA31D94F8]" . "Mikrosatelity nebo jednoduch\u00E9 sekven\u010Dn\u00ED opakov\u00E1n\u00ED (SSR) jsou velmi roz\u0161\u00ED\u0159en\u00E9 kategorie repetitivn\u00EDch sekvenc\u00ED DNA, kter\u00E9 se pou\u017E\u00EDvaj\u00ED v popula\u010Dn\u00ED genetice a studi\u00EDch mapov\u00E1n\u00ED genetick\u00E9 rozmanitosti. Genetick\u00E9 a evolu\u010Dn\u00ED mechanismy z pohledu SSR nejsou zcela objasn\u011Bny. T\u0159i mikrosatelitn\u00ED lokusy s r\u016Fznou pozic\u00ED v genomu hrachu (Pisum sativum L.), A9 aktivn\u00ED lokus v LTR oblasti bohat\u00E9 retrotransposony, AD270 a AF016458 se nach\u00E1zej\u00EDc\u00ED se v oblasti genu 5'. Srovn\u00E1vac\u00ED anal\u00FDza 35 p\u00E1r\u016F vzork\u016F od sedmi odr\u016Fd vyp\u011Bstovan\u00FDch z jednoho semene po deset generac\u00ED, odhalila jednu 4 bp mutaci v 10. generaci vzorku v AD270 lokusu odpov\u00EDdaj\u00EDc\u00ED skokov\u00E9mu n\u00E1r\u016Fstu v jedn\u00E9 dal\u0161\u00ED ATCT jednotce opakov\u00E1n\u00ED.Odhadovan\u00E1 m\u00EDra mutace byla 4,76 x 10 (-3) na lokus a na generaci, s 95% intervalem spolehlivosti 1,2 x 10 (-4) a\u017E 2,7 x 10 (-2). Srovn\u00E1vac\u00ED polo\u017Eky odr\u016Fdy Bohat\u00FDr z\u00EDskan\u00E9 z r\u016Fzn\u00FDch kolekc\u00ED genov\u00FDch zdroj\u016F , uk\u00E1zaly intra a inter-odr\u016Fdovou variabilitu v doprovodn\u00FDch a opakovan\u00FDch sekvenc\u00EDch. Rozd\u00EDly ve velikosti fragmentu a st\u0159\u00EDd\u00E1n\u00ED sekvenc\u00ED byly nalezeny i v dlouhodob\u00E9m horizontu v in vitro organogenn\u00ED kultu\u0159e, zalo\u017Een\u00E9 v roce 1983 sv\u011Bd\u010D\u00EDc\u00ED o somatick\u00E9m muta\u010Dn\u00EDm procesu. D\u016Fkaz homoplasie byl zji\u0161t\u011Bn v r\u00E1mci nez\u00E1visl\u00FDch genotyp\u016F hrachu, kter\u00E1 nep\u0159\u00EDzniv\u011B ovliv\u0148uje spolehlivost genov\u00FDch zdroj\u016F, ale tak\u00E9 vysoce pro\u0161lecht\u011Bn\u00E9ho materi\u00E1l\u016F. Z\u00E1v\u011Bry t\u00E9to studie maj\u00ED d\u016Fle\u017Eit\u00FD v\u00FDznam pro studium fylogeneze rodu Pisum, ur\u010Den\u00ED odr\u016Fdy a postup registrace odr\u016Fd hrachu, kde m\u00EDra mutace ovliv\u0148uje genetickou rozmanitost a efektivn\u00EDch odhady velikosti v\u00FDchoz\u00ED populace." . "000295682700002" . . . "H\u00FDbl, Miroslav" . . . "5"^^ . . . . . .