. . "Pro zv\u00FD\u0161en\u00ED procenta transformovan\u00FDch pletiv na explant\u00E1tech po kokultivaci s Agrobacteriem byl sledov\u00E1n vliv odstran\u011Bn\u00ED epidermis z hypokotylu, vliv r\u016Fzn\u00E9 doby kokultivace a r\u016Fzn\u00FDch koncentrac\u00ED acetosyringonu. Efektivita transformace byla porovn\u00E1v\u00E1na na z\u00E1klad\u011B GUS zabarven\u00ED explant\u00E1t\u016F po 3 t\u00FDdnech od kokultivace pomoc\u00ED obrazov\u00E9 anal\u00FDzy. Na z\u00E1klad\u011B statistick\u00E9ho hodnocen\u00ED v\u00FDsledk\u016F obrazov\u00E9 anal\u00FDzy byla vyhodnocena jako neefektivn\u011Bj\u0161\u00ED koncentrace 100mg/l acetosyringonu a doba trv\u00E1n\u00ED kokultivace 10 minut. Odstran\u011Bn\u00ED epidermis zv\u00FD\u0161ilo procento transformovan\u00FDch pletiv o 11,75%."@cs . "\u0160v\u00E1bov\u00E1, Lenka" . "2008-01-01+01:00"^^ . . "Griga, Miroslav" . . . "6"^^ . "Improved transformation methodology for aquirement of stably transformed flax (Linum usitatissumum L.)."@en . "Vrbov\u00E1, Miroslava" . "Improved transformation methodology for aquirement of stably transformed flax (Linum usitatissumum L.)."@en . "978-80-7375-234-7" . . . . . "Zdokonalen\u00ED transforma\u010Dn\u00ED metodiky pro z\u00EDsk\u00E1n\u00ED stabiln\u011B transformovan\u00E9ho lnu."@cs . "P(1M06030), Z(MSM2678424601)" . "[8143256ABC03]" . . "Improved transformation methodology for aquirement of stably transformed flax (Linum usitatissumum L.)." . "Improved transformation methodology for aquirement of stably transformed flax (Linum usitatissumum L.)." . . "Funk\u010Dn\u00ED genomika a proteomika ve \u0161lecht\u011Bn\u00ED rostlin." . . "Sm\u00FDkal, Petr" . . . "Zdokonalen\u00ED transforma\u010Dn\u00ED metodiky pro z\u00EDsk\u00E1n\u00ED stabiln\u011B transformovan\u00E9ho lnu."@cs . "5"^^ . . . . "K\u0159tiny, 9-10.9.2008" . . "RIV/26784246:_____/08:#0000103" . . "Vinkl\u00E1rkov\u00E1, Petr" . . "RIV/26784246:_____/08:#0000103!RIV09-MSM-26784246" . "5"^^ . . "MZLU, Brno, \u010Cesk\u00E1 Republika" . "Agrobacterium- mediated transformation, acetosyringone, flax"@en . "To increase the percentage of the transformed tissue explants after cocultivation with Agrobacterium has been monitoring the influence of the removal of the hypocotyl epidermis , the influence of different time cocutivation and various acetosyringone concentrations. Efficiency of transformation was compared on the basis of GUS exxpresion on explants after 3 weeks from cocultivation using image analysis. Based on the statistical evaluation of the results of image analysis was evaluated he concentration of 100mg/l acetosyringonu as the most effecient and time of duration cocultivation 10 minutes. Removal of the epidermis increased the percentage of transformed tissue on 11,75%." . "MZLU, Brno, \u010Cesk\u00E1 Republika" . "To increase the percentage of the transformed tissue explants after cocultivation with Agrobacterium has been monitoring the influence of the removal of the hypocotyl epidermis , the influence of different time cocutivation and various acetosyringone concentrations. Efficiency of transformation was compared on the basis of GUS exxpresion on explants after 3 weeks from cocultivation using image analysis. Based on the statistical evaluation of the results of image analysis was evaluated he concentration of 100mg/l acetosyringonu as the most effecient and time of duration cocultivation 10 minutes. Removal of the epidermis increased the percentage of transformed tissue on 11,75%."@en . . "371701" .