"We have used two highly abundant retrotransposons, representative one of the major group of long terminal repeats (LTR) - containing retrotransposons of Ty3/gypsy-like class, Ogre and Cyclop, for development of molecular markers for pea cultivars genotyping. The aim of this study was to access retrotransposon based markers to evaluate the genetic diversity of pea (Pisum sativum L.). To test the utility and reliability of the method we have chosen 20 commercially used pea cultivars with well known pedegree. Due to the sequence knowledge, IRAP markers are better scorable and result in more accurate genetic distance estimations over the REMAP, ISSR and RAPD techniques. With the use of just two outward-facing primers annealing to LTR Ogre and Cyclop target sequences 56 and 43 polymorphic bands per cultivar and reaction were produced which clearly distinguished and separated 20 investigated pea cultivars into groups related to known pedegree." . . "Vienna, Rakousko" . "1"^^ . "2004-01-01+01:00"^^ . "[12DCB6513873]" . . . "3-900962-56-1" . . . "We have used two highly abundant retrotransposons, representative one of the major group of long terminal repeats (LTR) - containing retrotransposons of Ty3/gypsy-like class, Ogre and Cyclop, for development of molecular markers for pea cultivars genotyping. The aim of this study was to access retrotransposon based markers to evaluate the genetic diversity of pea (Pisum sativum L.). To test the utility and reliability of the method we have chosen 20 commercially used pea cultivars with well known pedegree. Due to the sequence knowledge, IRAP markers are better scorable and result in more accurate genetic distance estimations over the REMAP, ISSR and RAPD techniques. With the use of just two outward-facing primers annealing to LTR Ogre and Cyclop target sequences 56 and 43 polymorphic bands per cultivar and reaction were produced which clearly distinguished and separated 20 investigated pea cultivars into groups related to known pedegree."@en . "Vyu\u017Eitelnost vysoce kopiov\u00FDch retrotransposonov\u00FDch element\u016F Ogre a cyclop pro molekul\u00E1rn\u00ED genotypizaci kolekce hrachu."@cs . "Utility of high copy number Ogre/Cyclop retrotransposons for molecular genotyping of pea (Pisum sativum L.) germplasm."@en . . "pea, genetic diversity, fingerprinting, retrotransposon"@en . "Utility of high copy number Ogre/Cyclop retrotransposons for molecular genotyping of pea (Pisum sativum L.) germplasm."@en . "591837" . . "H\u00FDbl, Miroslav" . "4"^^ . . . "Vyu\u017Eitelnost vysoce kopiov\u00FDch retrotransposonov\u00FDch element\u016F Ogre a cyclop pro molekul\u00E1rn\u00ED genotypizaci kolekce hrachu."@cs . "Genetic Variation for Plant Breeding" . "Z(MSM2678424601)" . "RIV/26784246:_____/04:#0000005!RIV07-MSM-26784246" . "4"^^ . . . "326" . . . . . . "Tulln , Rakousko" . "Pomoc\u00ED dvou vybran\u00FDch retrotransposon\u016F Ogre a Cyclop byla vyvinuta metoda vhodn\u00E1 pro studium genetick\u00E9 struktury genofondov\u00FDch sb\u00EDrek harchu. V\u00FDhoda t\u00E9to IRAP techniky ve srovn\u00E1n\u00ED v jin\u00FDmi je vysok\u00E1 specificita a zisk dostate\u010Dn\u00E9ho mno\u017Estv\u00ED informac\u00ED posta\u010Duj\u00EDc\u00EDch k odli\u0161en\u00ED i bl\u00EDzce p\u0159\u00EDbuzn\u00FDch odr\u016Fd hrachu"@cs . "Sm\u00FDkal, Petr" . "Utility of high copy number Ogre/Cyclop retrotransposons for molecular genotyping of pea (Pisum sativum L.) germplasm." . . . . "Griga, Miroslav" . "Dost\u00E1lov\u00E1, Radmila" . "Utility of high copy number Ogre/Cyclop retrotransposons for molecular genotyping of pea (Pisum sativum L.) germplasm." . "BOKU University, Tull" . "RIV/26784246:_____/04:#0000005" . .