"Molecular-biological and cultivation methods for detection and enumeration of B. cereus"@en . . "Molekul\u00E1rn\u011B-biologick\u00E9 a kultiva\u010Dn\u00ED metody detekce a stanoven\u00ED B. cereus" . . "RIV/26722861:_____/10:HYGIEN10!RIV11-MSM-26722861" . "N\u011Bme\u010Dkov\u00E1, Irena" . . "P(2B06048), Z(MSM2672286101)" . "1"^^ . "Molekul\u00E1rn\u011B-biologick\u00E9 a kultiva\u010Dn\u00ED metody detekce a stanoven\u00ED B. cereus"@cs . . . "Bacillus cereus; cultivation methods; PCR methods"@en . . "Molekul\u00E1rn\u011B-biologick\u00E9 a kultiva\u010Dn\u00ED metody detekce a stanoven\u00ED B. cereus" . . "RIV/26722861:_____/10:HYGIEN10" . "Hlavn\u00EDm c\u00EDlem t\u00E9to pr\u00E1ce je porovnat kultiva\u010Dn\u00ED metody stanoven\u00ED spor B. cereus v ml\u00E9ce a potvrdit v\u00FDsledky pomoc\u00ED PCR metod. 70 vzork\u016F syrov\u00E9ho kravsk\u00E9ho ml\u00E9ka bylo inaktivov\u00E1no z\u00E1h\u0159evem 85 \u00B0C/10 min a pot\u00E9 o\u010Dkov\u00E1no na referen\u010Dn\u00ED p\u016Fdu (MYPA) a na testovan\u00E9 p\u016Fdy (PEMBA a dv\u011B chromogenn\u00ED p\u016Fdy - BrillianceTM Bacillus cereus agar, HiCrome Bacillus agar) a kultivov\u00E1no 30 \u00B0C/24 \u2013 48 h. Jak pozitivn\u00ED, tak negativn\u00ED kolonie byly p\u0159eneseny z jedn\u00E9 na ostatn\u00ED p\u016Fdy a netypick\u00E9 kolonie suspektn\u00EDho B. cereus byly izolov\u00E1ny a konfirmov\u00E1ny PCR metodami. Potvrzena byla p\u0159\u00EDslu\u0161nost k rodu Bacillus, av\u0161ak p\u0159\u00EDslu\u0161nost k druhu B. cereus nebyla s navr\u017Een\u00FDmi primery prok\u00E1z\u00E1na. V\u0161echny kultiva\u010Dn\u00ED metody se kvantitativn\u011B osv\u011Bd\u010Dily, av\u0161ak li\u0161ily se v po\u017Eadavc\u00EDch na zku\u0161enost laboratorn\u00EDho person\u00E1lu. Hygiena Alimentorum XXXI \u201EBezpe\u010Dnos\u0165 a kvalita mlieka\u201C, kv\u011Bten 2010, \u0160trbsk\u00E9 Pleso, Vysok\u00E9 Tatry. Sborn\u00EDk str. 208 \u2013 210. ISBN 978-80-8077-186-7" . . . "Uhrov\u00E1, B." . "Plockov\u00E1, M." . . "Solichov\u00E1, K." . "Molecular-biological and cultivation methods for detection and enumeration of B. cereus"@en . "Molekul\u00E1rn\u011B-biologick\u00E9 a kultiva\u010Dn\u00ED metody detekce a stanoven\u00ED B. cereus"@cs . "Roubal, Petr" . . "272407" . "The main aim of this work is to compare cultivation plate methods for enumeration of B. cereus spores in milk and to confirm results by PCR methods. 70 samples of raw cow milk were inactivated at 85 \u00B0C for 10 min and then inoculated on standard medium (MYPA) and tested media (PEMBA and two chromogenic media \u2013 BrillianceTM Bacillus cereus agar, HiCrome Bacillus agar) and incubated at 30 \u00B0C for 24 \u2013 48 hrs. Both positive and negative colonies were transferred from one to each other media and B. cereus-like but non-typical colonies were isolated and genus confirmed by PCR method only as bacilli, their species identification as B. cereus was not by designed primers confirmed. All cultivation methods approved themselves quantitatively but differences were found in demands on laboratory workers experience. Hygiena Alimentorum XXXI \u201EBezpe\u010Dnos\u0165 a kvalita mlieka\u201C, kv\u011Bten 2010, \u0160trbsk\u00E9 Pleso, Vysok\u00E9 Tatry. Sborn\u00EDk str. 208 \u2013 210. ISBN 978-80-8077-186-7"@en . . . . . "[BE7521082134]" . "Hlavn\u00EDm c\u00EDlem t\u00E9to pr\u00E1ce je porovnat kultiva\u010Dn\u00ED metody stanoven\u00ED spor B. cereus v ml\u00E9ce a potvrdit v\u00FDsledky pomoc\u00ED PCR metod. 70 vzork\u016F syrov\u00E9ho kravsk\u00E9ho ml\u00E9ka bylo inaktivov\u00E1no z\u00E1h\u0159evem 85 \u00B0C/10 min a pot\u00E9 o\u010Dkov\u00E1no na referen\u010Dn\u00ED p\u016Fdu (MYPA) a na testovan\u00E9 p\u016Fdy (PEMBA a dv\u011B chromogenn\u00ED p\u016Fdy - BrillianceTM Bacillus cereus agar, HiCrome Bacillus agar) a kultivov\u00E1no 30 \u00B0C/24 \u2013 48 h. Jak pozitivn\u00ED, tak negativn\u00ED kolonie byly p\u0159eneseny z jedn\u00E9 na ostatn\u00ED p\u016Fdy a netypick\u00E9 kolonie suspektn\u00EDho B. cereus byly izolov\u00E1ny a konfirmov\u00E1ny PCR metodami. Potvrzena byla p\u0159\u00EDslu\u0161nost k rodu Bacillus, av\u0161ak p\u0159\u00EDslu\u0161nost k druhu B. cereus nebyla s navr\u017Een\u00FDmi primery prok\u00E1z\u00E1na. V\u0161echny kultiva\u010Dn\u00ED metody se kvantitativn\u011B osv\u011Bd\u010Dily, av\u0161ak li\u0161ily se v po\u017Eadavc\u00EDch na zku\u0161enost laboratorn\u00EDho person\u00E1lu. Hygiena Alimentorum XXXI \u201EBezpe\u010Dnos\u0165 a kvalita mlieka\u201C, kv\u011Bten 2010, \u0160trbsk\u00E9 Pleso, Vysok\u00E9 Tatry. Sborn\u00EDk str. 208 \u2013 210. ISBN 978-80-8077-186-7"@cs . . "5"^^ . .