"Odli\u0161en\u00ED kmen\u016F druhu L.helveticus od fylogeneticky p\u0159\u00EDbuzn\u00FDch druh\u016F L.delbrueckii a L.acidophilus pomoc\u00ED biochemick\u00FDch test\u016F a molekul\u00E1rn\u011B genetick\u00FDch metod"@cs . "Vil\u00EDmkov\u00E1, Miroslava" . . "5"^^ . "The differentiation of the Lactobacillus helveticus strains from phylogenetically related species Lactobacillus delbrueckii and Lactobacillusacidophilus by means of biochemical tests and genotyping"@en . . "Total 41 strains of thermophilic lactobacilli, stored in the Collection of Dairy Microorganisms Lactoflora (CCDM) and employed in dairy industry mainly forcheese and yogurt production, were identified by means of the combination biochemical (API 50 CH) and genotypic methods (species specific PCR for L helveticus), ribotyping. Biochemical identification using commercial set API 50 CH did not allow reliably differentiation of L. helveticus from L. delbrueckii and of complex L. acidophilus. Species specific PCR for L. helveticus was positive for 30 strains. Because specific PCR products were also detected for phylogenetically related species L. gallinarium CCM 4383 T, the correct taxonomic classification was confirmed by means of ribotyping with restriction endonuclease EcoRI. The other 4 strains were classified as L. delbrueckii. Used typing techniques didn\u00B4t allow classify 7 strains."@en . "Dr\u00E1b, Vladim\u00EDr" . . "[C3570258B576]" . "\u0160vec, Pavel" . . "RIV/26722861:_____/08:ODLISE08!RIV09-MSM-26722861" . . . "108" . . "Z(MSM2672286101)" . "384275" . "Celkem 41 kmen\u016F termofiln\u00EDch laktobacil\u016F ulo\u017Een\u00FDch ve Sb\u00EDrce ml\u00E9k\u00E1rensk\u00FDch mikroorganism\u016F Laktoflora (CCDM) a vyu\u017E\u00EDvan\u00FDch v ml\u00E9k\u00E1rensk\u00E9m pr\u016Fmyslu p\u0159ev\u00E1\u017En\u011B pro v\u00FDrobu s\u00FDr\u016F a jogurt\u016F bylo identifikov\u00E1no pomoc\u00ED kombinace biochemick\u00FDch (API 50 CH) a genotypov\u00FDch metod (druhov\u011B specifick\u00E1 PCR pro L. helveticus, ribotypizace). Biochemick\u00E1 identifikace pomoc\u00ED komer\u010Dn\u00ED soupravy API 50 CH neumo\u017Enila spolehliv\u00E9 rozli\u0161en\u00ED L. helveticus od L. delbrueckii a komplexu L. acidophilus. Druhov\u011B specifick\u00E1 PCR pro L. helveticus byla pozitivn\u00ED pro 30 analyzovan\u00FDch kmen\u016F. Vzhledem k detekci specifick\u00FDch PCR produkt\u016F tak\u00E9 u kmene L. gallinarum CCM 4383T (fylogeneticky bl\u00EDzce p\u0159\u00EDbuzn\u00FD druh), bylo za\u0159azen\u00ED kmen\u016F do druhu L. helveticus ov\u011B\u0159eno ribotypizac\u00ED s restrik\u010Dn\u00EDm enzymem EcoRI. Ribotypizac\u00ED bylo potvrzeno za\u0159azen\u00ED kmen\u016F do druhu L. helveticus. Dal\u0161\u00ED 4 kmeny byly za\u0159azeny do druhu L. delbrueckii. Celkem 7 kmen\u016F nebylo mo\u017En\u00E9 pomoc\u00ED pou\u017Eit\u00FDch metod taxonomicky za\u0159adit."@cs . "The differentiation of the Lactobacillus helveticus strains from phylogenetically related species Lactobacillus delbrueckii and Lactobacillusacidophilus by means of biochemical tests and genotyping"@en . . . . . "CZ - \u010Cesk\u00E1 republika" . "Celkem 41 kmen\u016F termofiln\u00EDch laktobacil\u016F ulo\u017Een\u00FDch ve Sb\u00EDrce ml\u00E9k\u00E1rensk\u00FDch mikroorganism\u016F Laktoflora (CCDM) a vyu\u017E\u00EDvan\u00FDch v ml\u00E9k\u00E1rensk\u00E9m pr\u016Fmyslu p\u0159ev\u00E1\u017En\u011B pro v\u00FDrobu s\u00FDr\u016F a jogurt\u016F bylo identifikov\u00E1no pomoc\u00ED kombinace biochemick\u00FDch (API 50 CH) a genotypov\u00FDch metod (druhov\u011B specifick\u00E1 PCR pro L. helveticus, ribotypizace). Biochemick\u00E1 identifikace pomoc\u00ED komer\u010Dn\u00ED soupravy API 50 CH neumo\u017Enila spolehliv\u00E9 rozli\u0161en\u00ED L. helveticus od L. delbrueckii a komplexu L. acidophilus. Druhov\u011B specifick\u00E1 PCR pro L. helveticus byla pozitivn\u00ED pro 30 analyzovan\u00FDch kmen\u016F. Vzhledem k detekci specifick\u00FDch PCR produkt\u016F tak\u00E9 u kmene L. gallinarum CCM 4383T (fylogeneticky bl\u00EDzce p\u0159\u00EDbuzn\u00FD druh), bylo za\u0159azen\u00ED kmen\u016F do druhu L. helveticus ov\u011B\u0159eno ribotypizac\u00ED s restrik\u010Dn\u00EDm enzymem EcoRI. Ribotypizac\u00ED bylo potvrzeno za\u0159azen\u00ED kmen\u016F do druhu L. helveticus. Dal\u0161\u00ED 4 kmeny byly za\u0159azeny do druhu L. delbrueckii. Celkem 7 kmen\u016F nebylo mo\u017En\u00E9 pomoc\u00ED pou\u017Eit\u00FDch metod taxonomicky za\u0159adit." . . "1212-950X" . . "6"^^ . "2008" . "strains of thermophilic lactobacilli"@en . "3"^^ . "Odli\u0161en\u00ED kmen\u016F druhu L.helveticus od fylogeneticky p\u0159\u00EDbuzn\u00FDch druh\u016F L.delbrueckii a L.acidophilus pomoc\u00ED biochemick\u00FDch test\u016F a molekul\u00E1rn\u011B genetick\u00FDch metod"@cs . "Kle\u010Dack\u00E1, Jana" . "Ml\u00E9ka\u0159sk\u00E9 listy zpravodaj" . . "Sedl\u00E1\u010Dek, Ivo" . . "Odli\u0161en\u00ED kmen\u016F druhu L.helveticus od fylogeneticky p\u0159\u00EDbuzn\u00FDch druh\u016F L.delbrueckii a L.acidophilus pomoc\u00ED biochemick\u00FDch test\u016F a molekul\u00E1rn\u011B genetick\u00FDch metod" . "RIV/26722861:_____/08:ODLISE08" . "Odli\u0161en\u00ED kmen\u016F druhu L.helveticus od fylogeneticky p\u0159\u00EDbuzn\u00FDch druh\u016F L.delbrueckii a L.acidophilus pomoc\u00ED biochemick\u00FDch test\u016F a molekul\u00E1rn\u011B genetick\u00FDch metod" .