"miRNA, EUS-FNA, Pancreatic Cancer"@en . . "The optimization of procedures for the quantification of prognostic and diagnostic miRNAs in pancreatic cancer from samples gained by EUS-FNA"@en . "\u010Cuperkov\u00E1, Romana" . . "OPTIMALIZACE POSTUP\u016E PRO KVANTIFIKACI PROGNOSTICK\u00DDCH A DIAGNOSTICK\u00DDCH MIRNA U KARCINOMU PANKREATU ZE VZORK\u016E Z\u00CDSKAN\u00DDCH METODOU EUS-FNA"@cs . "Min\u00E1rik, Marek" . "The optimization of procedures for the quantification of prognostic and diagnostic miRNAs in pancreatic cancer from samples gained by EUS-FNA"@en . . . "RIV/26475821:_____/13:#0000185!RIV14-MZ0-26475821" . "Bene\u0161ov\u00E1, Lucie" . . . . "Bel\u0161\u00E1nov\u00E1, Barbora" . "7"^^ . . "RIV/26475821:_____/13:#0000185" . "OPTIMALIZACE POSTUP\u016E PRO KVANTIFIKACI PROGNOSTICK\u00DDCH A DIAGNOSTICK\u00DDCH MIRNA U KARCINOMU PANKREATU ZE VZORK\u016E Z\u00CDSKAN\u00DDCH METODOU EUS-FNA"@cs . . . "OPTIMALIZACE POSTUP\u016E PRO KVANTIFIKACI PROGNOSTICK\u00DDCH A DIAGNOSTICK\u00DDCH MIRNA U KARCINOMU PANKREATU ZE VZORK\u016E Z\u00CDSKAN\u00DDCH METODOU EUS-FNA" . "94349" . . "Poster: Pancreatic cancer (CP) is a very serious disease with most infaust prognosis (average 5-year survival rate <5%), a high mortality rate and increasing incidence in the population. Currently there is no reliable tool or marker, which can diagnose the disease in its early stages with a reasonable specificity and sensitivity. Patients with CP at the time of diagnosis are often in poor health and treatment does not usually have a significant effect. For these reasons, more and more emphasis on finding suitable molecular markers and in particular to improve the diagnosis and estimation of prognosis. One of these markers are microRNAs, 21-23 nt long single-stranded noncoding RNA, potentially useful in both diagnosis and prognosis, as well as to monitor the course of disease, prediction of response to treatment or the design of new therapies. Since it is only a few patients indicated for surgery, sampling for subsequent examination is carried by fine needle biopsy under control endoscopic ultrasonography (EUS-FNA) in order to confirm the diagnosis of CP or chronic pancreatitis. Normally, these samples are processed only as cytological preparations, but more complex molecular genetic testing, such as determining the levels of miRNA, require larger amounts of tissue. Taking a such sample is not performed in a routine practice, and it requires in addition to skill and experience of the physician, the choice of appropriate biopsy needle and sampling techniques. In addition, the success of molecular analyzes of pancreatic tissue is lower compared to other tissues and thus the right choice procedure of isolation of nucleic acids (NA) and subsequent analyzes including the kits is crucial. The aim of this work was to optimize the whole process from the sampling over NA isolation and cDNA synthesis to analysis of KRAS mutations and levels of selected miRNAs from pancreatic tissue samples obtained by EUS-FNA to find prognostic and diagnostic miRNA."@en . . . "4"^^ . "Karcinom pankreatu (CP) je velmi z\u00E1va\u017En\u00E9 onemocn\u011Bn\u00ED s v\u011Bt\u0161inou infaustn\u00ED progn\u00F3zou (pr\u016Fm\u011Brn\u00E9 5-let\u00E9 p\u0159e\u017Eit\u00ED < 5%), vysokou mortalitou a zvy\u0161uj\u00EDc\u00ED se incidenc\u00ED v populaci. V sou\u010Dasn\u00E9 dob\u011B neexistuje \u017E\u00E1dn\u00FD spolehliv\u00FD n\u00E1stroj ani marker, kter\u00FD by s dostate\u010Dnou specifitou a citlivost\u00ED diagnostikoval toto onemocn\u011Bn\u00ED v jeho \u010Dasn\u00FDch st\u00E1di\u00EDch. Pacienti s CP v dob\u011B diagn\u00F3zy tak b\u00FDvaj\u00ED \u010Dasto ve \u0161patn\u00E9m zdravotn\u00EDm stavu a l\u00E9\u010Dba nem\u00EDv\u00E1 v\u00FDrazn\u00FD \u00FA\u010Dinek. Z t\u011Bchto d\u016Fvod\u016F se klade st\u00E1le v\u011Bt\u0161\u00ED d\u016Fraz na nalezen\u00ED vhodn\u00FDch molekul\u00E1rn\u00EDch marker\u016F a to zejm\u00E9na pro zlep\u0161en\u00ED diagn\u00F3zy a odhad progn\u00F3zy. Jedn\u00EDm z takov\u00FDch marker\u016F jsou mikroRNA, 21-23 nt dlouh\u00E9 jedno\u0159et\u011Bzcov\u00E9 nek\u00F3duj\u00EDc\u00ED RNA, potencion\u00E1ln\u011B vyu\u017Eiteln\u00E9 jak v diagnostice a stanoven\u00ED progn\u00F3zy, tak i ke sledov\u00E1n\u00ED pr\u016Fb\u011Bhu onemocn\u011Bn\u00ED, predikci odpov\u011Bdi na l\u00E9\u010Dbu nebo n\u00E1vrhu nov\u00FDch l\u00E9\u010Debn\u00FDch terapi\u00ED. Vzhledem k tomu, \u017Ee je jen m\u00E1lo pacient\u016F indikov\u00E1no k operaci, odb\u011Br vzork\u016F pro n\u00E1sledn\u00E1 vy\u0161et\u0159en\u00ED se prov\u00E1d\u00ED biopsi\u00ED tenkou jehlou pod kontrolou endoskopick\u00E9 ultrasonografie (EUS-FNA) a to za \u00FA\u010Delem potvrzen\u00ED CP nebo stanoven\u00ED diagn\u00F3zy chronick\u00E1 pankreatitida. B\u011B\u017En\u011B jsou tyto vzorky zpracov\u00E1v\u00E1ny pouze jako cytologick\u00E9 prepar\u00E1ty, ale slo\u017Eit\u011Bj\u0161\u00ED molekul\u00E1rn\u011B-genetick\u00E1 vy\u0161et\u0159en\u00ED, jako je stanoven\u00ED hladin miRNA, vy\u017Eaduj\u00ED v\u011Bt\u0161\u00ED mno\u017Estv\u00ED tk\u00E1n\u011B. Odb\u011Br takov\u00E9ho vzorku se v rutinn\u00ED praxi neprov\u00E1d\u00ED a vy\u017Eaduje krom\u011B zru\u010Dnosti a zku\u0161enosti l\u00E9ka\u0159e tak\u00E9 volbu vhodn\u00E9 bioptick\u00E9 jehly a techniky odb\u011Bru. Nav\u00EDc \u00FAsp\u011B\u0161nost molekul\u00E1rn\u00EDch vy\u0161et\u0159en\u00ED pankreatick\u00E9 tk\u00E1n\u011B b\u00FDv\u00E1 ni\u017E\u0161\u00ED v porovn\u00E1n\u00ED s ostatn\u00EDmi tk\u00E1n\u011Bmi a tak spr\u00E1vn\u00E1 volba postupu izolace nukleov\u00FDch kyselin (NK) a n\u00E1sledn\u00FDch anal\u00FDz v\u010Detn\u011B pou\u017Eit\u00FDch kit\u016F je kl\u00ED\u010Dov\u00E1. C\u00EDlem t\u00E9to pr\u00E1ce bylo zoptimalizovat cel\u00FD postup od odb\u011Bru vzork\u016F, p\u0159es izolaci NK a synt\u00E9zu cDNA a\u017E po anal\u00FDzu mutac\u00ED genu KRAS a hladin vybran\u00FDch miRNA ze vzork\u016F tk\u00E1n\u011B pankreatu z\u00EDskan\u00FDch pomoc\u00ED EUS-FNA za \u00FA\u010Delem nalezen\u00ED prognostick\u00FDch a diagnostick\u00FDch miRNA."@cs . . "OPTIMALIZACE POSTUP\u016E PRO KVANTIFIKACI PROGNOSTICK\u00DDCH A DIAGNOSTICK\u00DDCH MIRNA U KARCINOMU PANKREATU ZE VZORK\u016E Z\u00CDSKAN\u00DDCH METODOU EUS-FNA" . . "Karcinom pankreatu (CP) je velmi z\u00E1va\u017En\u00E9 onemocn\u011Bn\u00ED s v\u011Bt\u0161inou infaustn\u00ED progn\u00F3zou (pr\u016Fm\u011Brn\u00E9 5-let\u00E9 p\u0159e\u017Eit\u00ED < 5%), vysokou mortalitou a zvy\u0161uj\u00EDc\u00ED se incidenc\u00ED v populaci. V sou\u010Dasn\u00E9 dob\u011B neexistuje \u017E\u00E1dn\u00FD spolehliv\u00FD n\u00E1stroj ani marker, kter\u00FD by s dostate\u010Dnou specifitou a citlivost\u00ED diagnostikoval toto onemocn\u011Bn\u00ED v jeho \u010Dasn\u00FDch st\u00E1di\u00EDch. Pacienti s CP v dob\u011B diagn\u00F3zy tak b\u00FDvaj\u00ED \u010Dasto ve \u0161patn\u00E9m zdravotn\u00EDm stavu a l\u00E9\u010Dba nem\u00EDv\u00E1 v\u00FDrazn\u00FD \u00FA\u010Dinek. Z t\u011Bchto d\u016Fvod\u016F se klade st\u00E1le v\u011Bt\u0161\u00ED d\u016Fraz na nalezen\u00ED vhodn\u00FDch molekul\u00E1rn\u00EDch marker\u016F a to zejm\u00E9na pro zlep\u0161en\u00ED diagn\u00F3zy a odhad progn\u00F3zy. Jedn\u00EDm z takov\u00FDch marker\u016F jsou mikroRNA, 21-23 nt dlouh\u00E9 jedno\u0159et\u011Bzcov\u00E9 nek\u00F3duj\u00EDc\u00ED RNA, potencion\u00E1ln\u011B vyu\u017Eiteln\u00E9 jak v diagnostice a stanoven\u00ED progn\u00F3zy, tak i ke sledov\u00E1n\u00ED pr\u016Fb\u011Bhu onemocn\u011Bn\u00ED, predikci odpov\u011Bdi na l\u00E9\u010Dbu nebo n\u00E1vrhu nov\u00FDch l\u00E9\u010Debn\u00FDch terapi\u00ED. Vzhledem k tomu, \u017Ee je jen m\u00E1lo pacient\u016F indikov\u00E1no k operaci, odb\u011Br vzork\u016F pro n\u00E1sledn\u00E1 vy\u0161et\u0159en\u00ED se prov\u00E1d\u00ED biopsi\u00ED tenkou jehlou pod kontrolou endoskopick\u00E9 ultrasonografie (EUS-FNA) a to za \u00FA\u010Delem potvrzen\u00ED CP nebo stanoven\u00ED diagn\u00F3zy chronick\u00E1 pankreatitida. B\u011B\u017En\u011B jsou tyto vzorky zpracov\u00E1v\u00E1ny pouze jako cytologick\u00E9 prepar\u00E1ty, ale slo\u017Eit\u011Bj\u0161\u00ED molekul\u00E1rn\u011B-genetick\u00E1 vy\u0161et\u0159en\u00ED, jako je stanoven\u00ED hladin miRNA, vy\u017Eaduj\u00ED v\u011Bt\u0161\u00ED mno\u017Estv\u00ED tk\u00E1n\u011B. Odb\u011Br takov\u00E9ho vzorku se v rutinn\u00ED praxi neprov\u00E1d\u00ED a vy\u017Eaduje krom\u011B zru\u010Dnosti a zku\u0161enosti l\u00E9ka\u0159e tak\u00E9 volbu vhodn\u00E9 bioptick\u00E9 jehly a techniky odb\u011Bru. Nav\u00EDc \u00FAsp\u011B\u0161nost molekul\u00E1rn\u00EDch vy\u0161et\u0159en\u00ED pankreatick\u00E9 tk\u00E1n\u011B b\u00FDv\u00E1 ni\u017E\u0161\u00ED v porovn\u00E1n\u00ED s ostatn\u00EDmi tk\u00E1n\u011Bmi a tak spr\u00E1vn\u00E1 volba postupu izolace nukleov\u00FDch kyselin (NK) a n\u00E1sledn\u00FDch anal\u00FDz v\u010Detn\u011B pou\u017Eit\u00FDch kit\u016F je kl\u00ED\u010Dov\u00E1. C\u00EDlem t\u00E9to pr\u00E1ce bylo zoptimalizovat cel\u00FD postup od odb\u011Bru vzork\u016F, p\u0159es izolaci NK a synt\u00E9zu cDNA a\u017E po anal\u00FDzu mutac\u00ED genu KRAS a hladin vybran\u00FDch miRNA ze vzork\u016F tk\u00E1n\u011B pankreatu z\u00EDskan\u00FDch pomoc\u00ED EUS-FNA za \u00FA\u010Delem nalezen\u00ED prognostick\u00FDch a diagnostick\u00FDch miRNA." . "P(NT13638)" . . "[2355FC45B900]" . . . .