. "\u00DAsp\u011B\u0161nost hybridizace souboru odr\u016Fd dvou\u0159ad\u00FDch jarn\u00EDch sladovnick\u00FDch je\u010Dmen\u016F r\u016Fzn\u00E9ho p\u016Fvodu byla ov\u011B\u0159ena prost\u0159ednictv\u00EDm kodominantn\u00EDho SSR (Simple Sequence Repeats) markeru HVWAXY, kter\u00FD je lokalizov\u00E1n v k\u00F3duj\u00EDc\u00ED sekvenci waxy genu. Kombinace primer\u016F F 5\u2019 AAG ACG TGG TGT TCG TGT G 3\u2019 a R 5\u2019 ATG GTT CCA GGG GTA AGT TC 3\u2019 generovala PCR produkt o velikosti 200 bp u odr\u016Fd sladovnick\u00E9ho je\u010Dmene Maridol, Bojos, Malz, Xanadu, Isotta, Josefin, Native, Sebastian, Conrad, KM 2436, KM 2629, KM-H-1320, kter\u00E9 byly pro k\u0159\u00ED\u017Een\u00ED pou\u017Eity jako mate\u0159sk\u00E9 a 250 bp u lini\u00ED KM 2416, Br 7571h33 pou\u017Eit\u00FDch jako komponent otcovsk\u00FD. Rodi\u010Dovsk\u00E9 genotypy nesouc\u00ED r\u016Fzn\u00E9 alely markeru byly k\u0159\u00ED\u017Eeny a k selekci \u0161t\u011Bp\u00EDc\u00EDho potomstva F3 generace byly pou\u017Eity molekul\u00E1rn\u00ED metody (marker-asisted selection). Tato p\u0159edselekce umo\u017Enila pracovat s ni\u017E\u0161\u00EDm po\u010Dtem jedinc\u016F (vy\u0159azen\u00EDm samoopylen\u00FDch jedinc\u016F) ji\u017E v po\u010D\u00E1te\u010Dn\u00ED f\u00E1zi \u0161lechtitelsk\u00E9ho procesu."@cs . "Z(MSM2532885901)" . . . . . "Pouch, Milan" . . . "Utilization of a molecular marker for evaluation of hybridization success in spring barley genetics and breeding" . . . . "[D7FB1D6F7273]" . "Utilization of a molecular marker for evaluation of hybridization success in spring barley genetics and breeding" . "43" . "5"^^ . "4" . "144-148" . "\u0160punarov\u00E1, Marie" . . . "RIV/25328859:_____/07:#0000225!RIV08-MSM-25328859" . . . . "RIV/25328859:_____/07:#0000225" . "Czech Journal of Genetics and Plant Breeding" . . "1212-1975" . "The success in hybridization of two-rowed spring barley genotypes of various origins was verified using a codominant SSR marker HVWAXY which is located in the coding sequence of the waxy gene. The primer pair F 5\u2019 AAG ACG TGG TGT TCG TGT G 3\u2019 and R 5\u2019 ATG GTT CCA GGG GTA AGT TC 3\u2019 generated the PCR product of approximately 200 bp in the spring barley genotypes Maridol, Bojos, Malz, Xanadu, Isotta, Josefin, Native, Sebastian, Conrad, KM 2436, KM 2439, KM 2629, KM-H-1320, whereas the product of about 250 bp was amplified in the breeding lines KM 2416 and Br 7571h33. The parental genotypes possessing different alleles of the marker were crossed and the segregating lines of the F3 generation were characterized employing molecular methods (marker-assisted selection). This prescreening enabled working with a lower number of individuals (selfed individuals were excluded) at the beginning stages of the breeding process leading to the acceleration of selection as a particular phase of breeding." . "\u0160punar, Jaroslav" . "3"^^ . "The success in hybridization of two-rowed spring barley genotypes of various origins was verified using a codominant SSR marker HVWAXY which is located in the coding sequence of the waxy gene. The primer pair F 5\u2019 AAG ACG TGG TGT TCG TGT G 3\u2019 and R 5\u2019 ATG GTT CCA GGG GTA AGT TC 3\u2019 generated the PCR product of approximately 200 bp in the spring barley genotypes Maridol, Bojos, Malz, Xanadu, Isotta, Josefin, Native, Sebastian, Conrad, KM 2436, KM 2439, KM 2629, KM-H-1320, whereas the product of about 250 bp was amplified in the breeding lines KM 2416 and Br 7571h33. The parental genotypes possessing different alleles of the marker were crossed and the segregating lines of the F3 generation were characterized employing molecular methods (marker-assisted selection). This prescreening enabled working with a lower number of individuals (selfed individuals were excluded) at the beginning stages of the breeding process leading to the acceleration of selection as a particular phase of breeding."@en . . "3"^^ . "457118" . "CZ - \u010Cesk\u00E1 republika" . . "Utilization of a molecular marker for evaluation of hybridization success in spring barley genetics and breeding"@en . "Vyu\u017Eit\u00ED molekul\u00E1rn\u00EDho markeru k hodnocen\u00ED \u00FAsp\u011Bchu k\u0159\u00ED\u017Een\u00ED v genetice a \u0161lecht\u011Bn\u00ED jarn\u00EDho je\u010Dmene"@cs . "Vyu\u017Eit\u00ED molekul\u00E1rn\u00EDho markeru k hodnocen\u00ED \u00FAsp\u011Bchu k\u0159\u00ED\u017Een\u00ED v genetice a \u0161lecht\u011Bn\u00ED jarn\u00EDho je\u010Dmene"@cs . "Utilization of a molecular marker for evaluation of hybridization success in spring barley genetics and breeding"@en . "molecular marker; hybridization; spring barley; breeding"@en .