"AFLP-based PCR markers that differentiate spot and net forms of Pyrenophora teres"@en . "[EA960D224CE5]" . "Mina\u0159\u00EDkov\u00E1, V\u011Bra" . "AFLP-based PCR markers that differentiate spot and net forms of Pyrenophora teres"@en . "Ku\u010Dera, Ladislav" . . "Specific polymerase chain reaction (PCR) primers were developed from amplified fragment length polymorphism (AFLP) fragments of Pyrenophora teres, the causal agent of net blotch on barley leaves. The primers were designed specifically to amplify DNA from P. teres f. teres (net form) and allow its differentiation from P. teres f. maculata (spot form), which is morphologically very similar to P. teres f. teres in culture. The PCR amplification was carried out successfully from DNA extracted from fungal mycelium. The PCR assay was validated with 60 samples of P. teres species. The amplification with four designed PCR primer pairs provided P. teres form-specific products. No cross-reaction was observed with DNA of several other species, such as P. tritici-repentis, P. graminea and Helminthosporium sativum." . . "Markery PCR zalo\u017Een\u00E9 na AFLP, kter\u00E9 rozli\u0161uj\u00ED skvrnitou a s\u00ED\u0165ovou formu Pyrenophora teres"@cs . . "RIV/25328859:_____/05:#0000035!RIV06-MSM-25328859" . . "66-73" . . "0032-0862" . "Specific polymerase chain reaction (PCR) primers were developed from amplified fragment length polymorphism (AFLP) fragments of Pyrenophora teres, the causal agent of net blotch on barley leaves. The primers were designed specifically to amplify DNA from P. teres f. teres (net form) and allow its differentiation from P. teres f. maculata (spot form), which is morphologically very similar to P. teres f. teres in culture. The PCR amplification was carried out successfully from DNA extracted from fungal mycelium. The PCR assay was validated with 60 samples of P. teres species. The amplification with four designed PCR primer pairs provided P. teres form-specific products. No cross-reaction was observed with DNA of several other species, such as P. tritici-repentis, P. graminea and Helminthosporium sativum."@en . "RIV/25328859:_____/05:#0000035" . . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . "Markery PCR zalo\u017Een\u00E9 na AFLP, kter\u00E9 rozli\u0161uj\u00ED skvrnitou a s\u00ED\u0165ovou formu Pyrenophora teres"@cs . . . "AFLP-based PCR markers that differentiate spot and net forms of Pyrenophora teres" . "1"^^ . . "AFLP-based PCR markers that differentiate spot and net forms of Pyrenophora teres" . "1" . "54" . "Byly vytvo\u0159eny primery PCR z AFLP fragment\u016F Pyrenophora teres, patogena hn\u011Bd\u00E9 skvrnitosti je\u010Dmene. Primery byly navr\u017Eeny tak, aby amplifikovaly DNA z P. teres f. teres (s\u00ED\u0165ov\u00E1 forma) a umo\u017Enily jej\u00ED odli\u0161en\u00ED od P. teres f. maculata (skvrnit\u00E1 forma), kter\u00E1 m\u00E1 kulturu morfologicky podobnou P. teres f. teres. Z DNA izolovan\u00E9 z mycelia houby byla \u00FAsp\u011B\u0161n\u011B provedena PCR amplifikace. Metoda PCR byla ov\u011B\u0159ena pomoc\u00ED 60 vzork\u016F druhu P. teres. Amplifikace \u010Dty\u0159 p\u00E1r\u016F PCR primer\u016F vytvo\u0159ila specifick\u00E9 produkty pro P. teres. Nebyla pozorov\u00E1na \u017E\u00E1dn\u00E1 k\u0159\u00ED\u017Eov\u00E1 reakce s DNA n\u011Bkolika jin\u00FDch druh\u016F, jako nap\u0159. P. tritici-repentis, P. graminea a Helminthosporium sativum."@cs . . . "4"^^ . "Ovesn\u00E1, Jaroslava" . "Plant Pathology" . "511521" . "PCR markers; Pyrenophora teres"@en . . "P(QC1361), Z(MSM2532885901)" . "8"^^ . . "Lei\u0161ov\u00E1, Leona" . . . .