"0231-6900" . "Papr\u0161tein, Franti\u0161ek" . "I" . . . "CZ - \u010Cesk\u00E1 republika" . . . . "explant; ribavirin; shoot; virus; medium"@en . . . "Svobodov\u00E1, Ladislava" . . . "RIV/25271121:_____/13:#0000491" . . "4"^^ . . . "Tal\u00E1cko, Lubo\u0161" . "RIV/25271121:_____/13:#0000491!RIV14-MZE-25271121" . "4"^^ . . . "23" . "In vitro thermotherapy and chemotherapy of apple cultivar \u00B4Jarka\u00B4"@en . "8"^^ . "In vitro termoterapie a chemoterapie odr\u016Fdy jablon\u011B \u00B4Jarka\u00B4"@cs . . "Apple cultivar \u00B4Jarka\u00B4 was selected for application of in vitro thermotherapy at 34 or 38 \u00B0C and chemotherapy on medium with 20 mg . l-1 of antivirotikum ribavirin. The presence of viruses in selected initial tree was detected by ELISA and RT-PCR testing before the beginning of sanitation procedures. Cultivar \u00B4Jarka\u00B4 was infected with Apple chlorotic leaf spot virus (ACLSV) and with Apple stem pitting virus (ASPV). \u00B4Jarka\u00B4 was successfully multiplied in in vitro cultures. Multiplication coefficient was 2.3 \u00B1 0.1 for \u00B4Jarka\u00B4 after four weeks of cultivation on MS medium with 1.5 mg . l-1 BAP. In the course of thermotherapy at 38 \u00B0C, the apical part of in vitro plants withered by the 5th day and viable shoots tips could not be taken. The heat treatment (34 \u00B0C) and chemotherapy (ribavirin 20 mg . l-1) were applied to the in vitro cultures on the same MS medium as for multiplication for the period of 30 days. After a culture period of 30 days, the apical part of the axis (about 3 mm in length comprising the apical meristem plus two-three primordial leaves) was dissected under a laminar flow hood and transferred to a fresh multiplication MS medium with 1.5 mg . l-1 BAP for regeneration. Twenty in vitro explants were taken in each treatment. After the end of chemotherapy and after repeated testing, 11 (55 %) clones of apple cultivar \u00B4Jarka\u00B4 were free of viruses discovered in initial plant. In contrary, only 1 (5 %) clone of \u00B4Jarka\u00B4 was virus-free after thermotherapy at 34 \u00B0C. Obtained results demonstrate the effectiveness of the system of virus elimination by a combination of in vitro cultures, chemotherapy with ribavirin and subsequent removing of apical meristematic region. Relatively high percentage of obtained virus free plants confirms high antiviral activity of ribavirin against ASPV and ACLSV."@en . . "[6582F3AFAF09]" . . "79413" . "In vitro termoterapie a chemoterapie odr\u016Fdy jablon\u011B \u00B4Jarka\u00B4" . "Odr\u016Fda jablon\u011B \u00B4Jarka\u00B4 byla vybr\u00E1na pro aplikaci in vitro termoterapie v 34 nebo 38 \u00B0C a chemoterapie na m\u00E9diu s 20 mg . l-1 antivirotika ribavirin. P\u0159\u00EDtomnost vir\u016F ve vybran\u00E9m v\u00FDchoz\u00EDm stromu byla p\u0159ed zah\u00E1jen\u00EDm ozdravov\u00E1n\u00ED zji\u0161\u0165ov\u00E1na metodou ELISA a RT-PCR. Bylo zji\u0161t\u011Bno, \u017Ee vybran\u00FD v\u00FDchoz\u00ED strom byl infikov\u00E1n virem chlorotick\u00E9 skvrnitosti jablon\u011B (Apple chlorotic leaf spot virus, ACLSV) a virem vr\u00E1s\u010Ditosti kmene jablon\u011B (Apple stem pitting virus, ASPV). Odr\u016Fda \u00B4Jarka\u00B4 byla \u00FAsp\u011B\u0161n\u011B namno\u017Eena v in vitro kultu\u0159e. Po \u010Dty\u0159ech t\u00FDdnech kultivace na MS m\u00E9diu s 1,5 mg . l-1 BAP byl dosa\u017Een multiplika\u010Dn\u00ED koeficient 2,3 \u00B1 0,1. V pr\u016Fb\u011Bhu termoterapie p\u0159i teplot\u011B 38 \u00B0C za\u010Dala odum\u00EDrat apik\u00E1ln\u00ED \u010D\u00E1st in vitro rostlin odr\u016Fdy ji\u017E do 5 dne, a z t\u011Bchto rostlin tak nemohly b\u00FDt odebr\u00E1ny \u017Eivotaschopn\u00E9 r\u016Fstov\u00E9 vrcholy. Teplotn\u00ED o\u0161et\u0159en\u00ED (34 \u00B0C) a chemoterapie (ribavirin 20 mg . l-1) byly u in vitro kultur po dobu 30 dn\u00ED aplikov\u00E1ny na stejn\u00E9m MS m\u00E9diu jako pro multiplikaci. Po t\u0159icetidenn\u00ED terapii byly vrcholov\u00E9 \u010D\u00E1sti velikosti okolo 3 mm obsahuj\u00EDc\u00ED meristematickou oblast se dv\u011Bma a\u017E t\u0159emi listov\u00FDmi primordii vypreparov\u00E1ny ve steriln\u00EDm prost\u0159ed\u00ED lamin\u00E1rn\u00EDho boxu a p\u0159eneseny na MS m\u00E9dium s 1,5 mg . l-1 BAP pro regeneraci. V ka\u017Ed\u00E9 variant\u011B terapie bylo odebr\u00E1no 20 in vitro explant\u00E1t\u016F. Na z\u00E1klad\u011B opakovan\u00E9ho testov\u00E1n\u00ED bylo po chemoterapii celkem z\u00EDsk\u00E1no 11 (55 %) meriklon\u016F a po termoterapii v 34 \u00B0C pouze 1 (5 %) meriklon odr\u016Fdy \u00B4Jarka\u00B4, kter\u00E9 se poda\u0159ilo ozdravit od vir\u016F, jimi\u017E byla infikov\u00E1na v\u00FDchoz\u00ED mate\u010Dn\u00E1 rostlina. Dosa\u017Een\u00E9 v\u00FDsledky potvrzuj\u00ED efektivitu syst\u00E9mu eliminace vir\u016F zalo\u017Een\u00E9 na kombinaci pou\u017Eit\u00ED in vitro kultury, chemoterapie s ribavirinem a n\u00E1sledn\u00E9m odb\u011Bru apik\u00E1ln\u00EDho meristematick\u00E9ho pletiva. Relativn\u011B vysok\u00FD pod\u00EDl z\u00EDskan\u00FDch bezvir\u00F3zn\u00EDch rostlin dokl\u00E1d\u00E1 vysokou antivirovou \u00FA\u010Dinnost ribavirinu proti ASPV a ACLSV."@cs . "Sedl\u00E1k, Ji\u0159\u00ED" . "In vitro thermotherapy and chemotherapy of apple cultivar \u00B4Jarka\u00B4"@en . "Odr\u016Fda jablon\u011B \u00B4Jarka\u00B4 byla vybr\u00E1na pro aplikaci in vitro termoterapie v 34 nebo 38 \u00B0C a chemoterapie na m\u00E9diu s 20 mg . l-1 antivirotika ribavirin. P\u0159\u00EDtomnost vir\u016F ve vybran\u00E9m v\u00FDchoz\u00EDm stromu byla p\u0159ed zah\u00E1jen\u00EDm ozdravov\u00E1n\u00ED zji\u0161\u0165ov\u00E1na metodou ELISA a RT-PCR. Bylo zji\u0161t\u011Bno, \u017Ee vybran\u00FD v\u00FDchoz\u00ED strom byl infikov\u00E1n virem chlorotick\u00E9 skvrnitosti jablon\u011B (Apple chlorotic leaf spot virus, ACLSV) a virem vr\u00E1s\u010Ditosti kmene jablon\u011B (Apple stem pitting virus, ASPV). Odr\u016Fda \u00B4Jarka\u00B4 byla \u00FAsp\u011B\u0161n\u011B namno\u017Eena v in vitro kultu\u0159e. Po \u010Dty\u0159ech t\u00FDdnech kultivace na MS m\u00E9diu s 1,5 mg . l-1 BAP byl dosa\u017Een multiplika\u010Dn\u00ED koeficient 2,3 \u00B1 0,1. V pr\u016Fb\u011Bhu termoterapie p\u0159i teplot\u011B 38 \u00B0C za\u010Dala odum\u00EDrat apik\u00E1ln\u00ED \u010D\u00E1st in vitro rostlin odr\u016Fdy ji\u017E do 5 dne, a z t\u011Bchto rostlin tak nemohly b\u00FDt odebr\u00E1ny \u017Eivotaschopn\u00E9 r\u016Fstov\u00E9 vrcholy. Teplotn\u00ED o\u0161et\u0159en\u00ED (34 \u00B0C) a chemoterapie (ribavirin 20 mg . l-1) byly u in vitro kultur po dobu 30 dn\u00ED aplikov\u00E1ny na stejn\u00E9m MS m\u00E9diu jako pro multiplikaci. Po t\u0159icetidenn\u00ED terapii byly vrcholov\u00E9 \u010D\u00E1sti velikosti okolo 3 mm obsahuj\u00EDc\u00ED meristematickou oblast se dv\u011Bma a\u017E t\u0159emi listov\u00FDmi primordii vypreparov\u00E1ny ve steriln\u00EDm prost\u0159ed\u00ED lamin\u00E1rn\u00EDho boxu a p\u0159eneseny na MS m\u00E9dium s 1,5 mg . l-1 BAP pro regeneraci. V ka\u017Ed\u00E9 variant\u011B terapie bylo odebr\u00E1no 20 in vitro explant\u00E1t\u016F. Na z\u00E1klad\u011B opakovan\u00E9ho testov\u00E1n\u00ED bylo po chemoterapii celkem z\u00EDsk\u00E1no 11 (55 %) meriklon\u016F a po termoterapii v 34 \u00B0C pouze 1 (5 %) meriklon odr\u016Fdy \u00B4Jarka\u00B4, kter\u00E9 se poda\u0159ilo ozdravit od vir\u016F, jimi\u017E byla infikov\u00E1na v\u00FDchoz\u00ED mate\u010Dn\u00E1 rostlina. Dosa\u017Een\u00E9 v\u00FDsledky potvrzuj\u00ED efektivitu syst\u00E9mu eliminace vir\u016F zalo\u017Een\u00E9 na kombinaci pou\u017Eit\u00ED in vitro kultury, chemoterapie s ribavirinem a n\u00E1sledn\u00E9m odb\u011Bru apik\u00E1ln\u00EDho meristematick\u00E9ho pletiva. Relativn\u011B vysok\u00FD pod\u00EDl z\u00EDskan\u00FDch bezvir\u00F3zn\u00EDch rostlin dokl\u00E1d\u00E1 vysokou antivirovou \u00FA\u010Dinnost ribavirinu proti ASPV a ACLSV." . "In vitro termoterapie a chemoterapie odr\u016Fdy jablon\u011B \u00B4Jarka\u00B4"@cs . "V\u011Bdeck\u00E9 pr\u00E1ce ovocn\u00E1\u0159sk\u00E9" . . . . "In vitro termoterapie a chemoterapie odr\u016Fdy jablon\u011B \u00B4Jarka\u00B4" .