. . "495488" . "Proliferation and cold hardening of in vitro grown apple shoot tips" . "micropropagation; germplasm; encapsulation; cryopreservation; freezing"@en . . "Debrecen" . . . "2004-09-12+02:00"^^ . "International Society for Horticultural Science" . . "000245292600066" . "Z\u00E1me\u010Dn\u00EDk, Ji\u0159\u00ED" . "RIV/25271121:_____/06:0R000945!RIV10-MZE-25271121" . "Sedl\u00E1k, Ji\u0159\u00ED" . . "Leuven" . . . . "Shoot tips of apple cv. Rubin were successfully established in vitro. Five proliferation MS (Murashige and Skoog) media containing different concentrations of BAP (0.25, 0.5, 1, 2 mg L-1) or a combination of 1 mg /L BAP and 0.1 mg /L IBA were tested. The highest proliferation rate was obtained on MS medium with 1 mg/ L BAP, which produced 4.2 +/- 0.3 shoots/explant after one month of cultivation. In vitro plants of cv. Rubin were cultivated for 4 weeks at low temperature (4 degrees C) and short photoperiod (8/16-h - light/dark). The assessment of frost resistance of cold hardened and non-hardened in vitro apple plants was done by freezing tests. The lethal temperature (LT) was 13.0 degrees C for cold hardened and -6.5 degrees C for non-hardened in vitro plants. Cold hardened in vitro cultures were used for subsequent cryopreservation. Cryopreservation was made by encapsulation-dehydration vitrification method. The survival and regeneration of cv. Rubin was 78% after thawing in 40 degrees C water." . "Bilav\u010D\u00EDk, Alois" . . "Proliferation and cold hardening of in vitro grown apple shoot tips"@en . . "978-90-6605-719-7" . . "[9D9BE7457F9D]" . "Acta Horticulturae" . "Papr\u0161tein, Franti\u0161ek" . "Shoot tips of apple cv. Rubin were successfully established in vitro. Five proliferation MS (Murashige and Skoog) media containing different concentrations of BAP (0.25, 0.5, 1, 2 mg L-1) or a combination of 1 mg /L BAP and 0.1 mg /L IBA were tested. The highest proliferation rate was obtained on MS medium with 1 mg/ L BAP, which produced 4.2 +/- 0.3 shoots/explant after one month of cultivation. In vitro plants of cv. Rubin were cultivated for 4 weeks at low temperature (4 degrees C) and short photoperiod (8/16-h - light/dark). The assessment of frost resistance of cold hardened and non-hardened in vitro apple plants was done by freezing tests. The lethal temperature (LT) was 13.0 degrees C for cold hardened and -6.5 degrees C for non-hardened in vitro plants. Cold hardened in vitro cultures were used for subsequent cryopreservation. Cryopreservation was made by encapsulation-dehydration vitrification method. The survival and regeneration of cv. Rubin was 78% after thawing in 40 degrees C water."@en . . . "P(QC1052)" . "4"^^ . "Proliferation and cold hardening of in vitro grown apple shoot tips" . "RIV/25271121:_____/06:0R000945" . . . . . "4"^^ . "Proliferation and cold hardening of in vitro grown apple shoot tips"@en . "2"^^ .