"\u0160imek, P." . "1186" . . . "Fluoroalkyl chloroformates in treating amino acids for gas chromatographic analysis" . "Novel fluoroalkyl chloroformates with 3 and 4 carbon atoms were investigated to immediate conversion of amino acids into hydrophobic derivatives in water-containing media. Derivatization conditions were extensively studied and optimized sample preparation protocols elaborated. More than 30 amino acids were treated with the particular reagent in isooctane by simply vortexing the reactive organic phase with a slightly basified aqueous medium containing pyridine or 3-picoline as a catalyst. Outstanding separation of nearly all components on 5% phenylmethylsilicone phase in gas chromatographic (GC) analysis with mass spectrometric (MS) or flame ionization detection (FID) required <10 min. Quantitation characteristics involving linearity in range of 0.1 to 100 nmol, MS limit of detection (LOD, 0.03 pmol - 20 pmol) and electron impact (EI) spectra and diagnostic SIM fragment ions of the derivatives are reported. The novel method is simple, robust and rapid, running in aqueous environment." . "Hu\u0161ek, Petr" . . . . "amino acids; derivatization; pentafluoropropyl- and heptafluorobutyl chloroformates; gas chromatography"@en . . "1"^^ . "000254884700034" . "P(GA203/04/0192), P(GA303/06/1674), Z(AV0Z50070508)" . . "Fluoroalkyl chloroformates in treating amino acids for gas chromatographic analysis" . . . "1-2" . "Fluoroalkyl chloroformates in treating amino acids for gas chromatographic analysis"@en . "RIV/00843989:_____/08:00100618!RIV10-GA0-00843989" . "RIV/00843989:_____/08:00100618" . "4"^^ . "10"^^ . "[6091F024E5BA]" . . "Zahradn\u00ED\u010Dkov\u00E1, H." . "Novel fluoroalkyl chloroformates with 3 and 4 carbon atoms were investigated to immediate conversion of amino acids into hydrophobic derivatives in water-containing media. Derivatization conditions were extensively studied and optimized sample preparation protocols elaborated. More than 30 amino acids were treated with the particular reagent in isooctane by simply vortexing the reactive organic phase with a slightly basified aqueous medium containing pyridine or 3-picoline as a catalyst. Outstanding separation of nearly all components on 5% phenylmethylsilicone phase in gas chromatographic (GC) analysis with mass spectrometric (MS) or flame ionization detection (FID) required <10 min. Quantitation characteristics involving linearity in range of 0.1 to 100 nmol, MS limit of detection (LOD, 0.03 pmol - 20 pmol) and electron impact (EI) spectra and diagnostic SIM fragment ions of the derivatives are reported. The novel method is simple, robust and rapid, running in aqueous environment."@en . . . "Hartvich, P." . "Journal of Chromatography A" . . "368246" . "0021-9673" . . . "NL - Nizozemsko" . . . "Fluoroalkyl chloroformates in treating amino acids for gas chromatographic analysis"@en . . .