"Influence of physiological and supraphysiological hyperinsulinemia on skin microcirculation in healthy volunteers"@en . "6"^^ . . "World Journal of Diabetes" . . "4" . "CN - \u010C\u00EDnsk\u00E1 lidov\u00E1 republika" . "\u010Cechurov\u00E1, Daniela" . . . . "7"^^ . "Jankovec, Zden\u011Bk" . "\u017Dourek, Michal" . "6" . "Influence of physiological and supraphysiological hyperinsulinemia on skin microcirculation in healthy volunteers" . . . "Kr\u010Dma, Michal" . "RIV/00669806:_____/13:10145795!RIV14-MZ0-00669806" . "7"^^ . . . . "I" . . "Influence of physiological and supraphysiological hyperinsulinemia on skin microcirculation in healthy volunteers"@en . "Microcirculation; Laser-doppler flowmetry; Insulin; Diabetes"@en . . "AIM To examine skin perfusion in dependency on insulinemia in healthy subjects. METHODS: All volunteers were informed in detail about the procedures and signed informed consent. The protocol of this study was approved by ethical committee. In our study a two-stage hyperinsulinemic euglycaemic clamp was performed, with insulinemia 100 and 250 mIU/mL and glycaemia 5.0 mmol/L (3% standard deviation). Before the clamp and in steady states, microcirculation was measured by laser-doppler flowmetry and transcutaneous oximetry and energy expenditure was measured by indirect calorimetry. Results (average and standard deviation) were evaluated with paired t-test. RESULTS: Physiological (50 mIU/L) insulinemia leaded to higher perfusion in both tests- hyperemia after heating to 44 oC- 1848% (984 - 2046) vs 1599% (801 - 1836), P < 0.05, half time of reaching peak perfusion after occlusion release 1.2 s (0.9 - 2.6) vs 4.9 s (1.8 - 11.4), P < 0.05. Supraphysiological (150 mIU/L) insulinemia leaded to even higher perfusion in both tests - hyperemia after heating to 44 oC - 1937% (1177 - 2488) vs 1599% (801 - 1836), P < 0.005, half time to reach peak perfusion after occlusion release 1.0 s (0.7 - 1.1) vs 4.9 s (1.8 - 11.4), P < 0.005. There occurred a statistically significant increase in tissue oxygenation in both insulinemia. The difference in perfusion and oxygenation between physiological and supraphysiological hyperinsulinemia were not statistically significant. CONCLUSION: Post occlusive hyperemia test in accordance with heating test showed significantly increasing skin perfusion in the course of artificial hyperinsulinemia. This effect rises non-linearly with increasing insulinemia. Dependency on the dose was not statistically significant."@en . . . "Influence of physiological and supraphysiological hyperinsulinemia on skin microcirculation in healthy volunteers" . "1948-9358" . "[8AC75D22103B]" . . "AIM To examine skin perfusion in dependency on insulinemia in healthy subjects. METHODS: All volunteers were informed in detail about the procedures and signed informed consent. The protocol of this study was approved by ethical committee. In our study a two-stage hyperinsulinemic euglycaemic clamp was performed, with insulinemia 100 and 250 mIU/mL and glycaemia 5.0 mmol/L (3% standard deviation). Before the clamp and in steady states, microcirculation was measured by laser-doppler flowmetry and transcutaneous oximetry and energy expenditure was measured by indirect calorimetry. Results (average and standard deviation) were evaluated with paired t-test. RESULTS: Physiological (50 mIU/L) insulinemia leaded to higher perfusion in both tests- hyperemia after heating to 44 oC- 1848% (984 - 2046) vs 1599% (801 - 1836), P < 0.05, half time of reaching peak perfusion after occlusion release 1.2 s (0.9 - 2.6) vs 4.9 s (1.8 - 11.4), P < 0.05. Supraphysiological (150 mIU/L) insulinemia leaded to even higher perfusion in both tests - hyperemia after heating to 44 oC - 1937% (1177 - 2488) vs 1599% (801 - 1836), P < 0.005, half time to reach peak perfusion after occlusion release 1.0 s (0.7 - 1.1) vs 4.9 s (1.8 - 11.4), P < 0.005. There occurred a statistically significant increase in tissue oxygenation in both insulinemia. The difference in perfusion and oxygenation between physiological and supraphysiological hyperinsulinemia were not statistically significant. CONCLUSION: Post occlusive hyperemia test in accordance with heating test showed significantly increasing skin perfusion in the course of artificial hyperinsulinemia. This effect rises non-linearly with increasing insulinemia. Dependency on the dose was not statistically significant." . "Lacigov\u00E1, Silvie" . "Bro\u017Eov\u00E1, Jitka" . . . "10.4239/wjd.v4.i6.372" . . . "Ru\u0161av\u00FD, Zden\u011Bk" . "79872" . . . "RIV/00669806:_____/13:10145795" . . "http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3874498/pdf/WJD-4-372.pdf" .