"RIV/00216305:26620/14:PU112643!RIV15-MSM-26620___" . "Kone\u010Dn\u00E1, Romana" . "[633E02E10A4A]" . "Doxorubicin Encapsulation Investigated by Capillary Electrophoresis with Laser- Induced Fluorescence Detection" . "RIV/00216305:26620/14:PU112643" . "7"^^ . "I, P(GAP301/10/0356)" . "0009-5893" . "26620" . . . . . . "12326" . "4"^^ . . "Doxorubicin Encapsulation Investigated by Capillary Electrophoresis with Laser- Induced Fluorescence Detection"@en . "Nguyen, Hoai Viet" . . . "Stiborov\u00E1, Marie" . "21-22" . . . "Capillary electrophoresis, Fluorescence, Drug delivery, Doxorubicin, Apoferritin"@en . "Doxorubicin (DOX) belongs to the group of anthracycline antibiotics with very effective anticancer properties. On the other hand, the cardiotoxic effects limit its application over the maximum cumulative dose. To overcome this obstacle, encapsulation of this drug into the protective nanotransporter such as apoferritin is beneficial. In this study, fluorescent behavior of DOX in various solvents was determined by fluorescence spectrometry, demonstrating the fluorescence quenching effect of water, which is often used as a solvent. It was found that by increasing the amount of the organic phase in the DOX solvent the dynamic quenching is significantly suppressed. Ethanol, acetonitrile and dimethyl sulfoxide were tested and the best linearity of the calibration curve was obtained when above 50 % of the solvent was present in the binary mixture with water. Moreover, pH influence on the DOX fluorescence was also observed within the range of 4\u201310. Two times higher fluorescence intensity was observed at pH"@en . . "Nguyen, Hoai Viet" . . "Bla\u017Ekov\u00E1, Iva" . "CHROMATOGRAPHIA" . . "Doxorubicin Encapsulation Investigated by Capillary Electrophoresis with Laser- Induced Fluorescence Detection" . "Doxorubicin (DOX) belongs to the group of anthracycline antibiotics with very effective anticancer properties. On the other hand, the cardiotoxic effects limit its application over the maximum cumulative dose. To overcome this obstacle, encapsulation of this drug into the protective nanotransporter such as apoferritin is beneficial. In this study, fluorescent behavior of DOX in various solvents was determined by fluorescence spectrometry, demonstrating the fluorescence quenching effect of water, which is often used as a solvent. It was found that by increasing the amount of the organic phase in the DOX solvent the dynamic quenching is significantly suppressed. Ethanol, acetonitrile and dimethyl sulfoxide were tested and the best linearity of the calibration curve was obtained when above 50 % of the solvent was present in the binary mixture with water. Moreover, pH influence on the DOX fluorescence was also observed within the range of 4\u201310. Two times higher fluorescence intensity was observed at pH" . "77" . . "Vaculovi\u010Dov\u00E1, Mark\u00E9ta" . "8"^^ . "DE - Spolkov\u00E1 republika N\u011Bmecko" . . . "K\u0159\u00ED\u017Ekov\u00E1, So\u0148a" . . . . "Stanisavljevic, Maja" . "Doxorubicin Encapsulation Investigated by Capillary Electrophoresis with Laser- Induced Fluorescence Detection"@en . .