"CHROMATOGRAPHIA" . "Gur\u00E1\u0148, Roman" . "Kizek, Ren\u00E9" . . "21-22" . "77" . "26620" . . "RIV/00216305:26620/14:PU112580" . "35716" . "Considerable efforts have been devoted to the development of rapid and sensitive methods allowing the detection of viral nucleic acid. We herein describe an assay for identification of a specific influenza sequence. The suggested method was based on isolation using paramagnetic particles coupled with electrochemical detection of isolated product. Peptide nucleic acid (PNA) was used as a probe for hybridization and identification of the influenza-derived specific sequence. The use of PNA can show numerous benefits: PNA probe is not degradable by enzymes and the duplex of PNA with RNA/DNA is more thermostable and more resistant to pH changes than DNA/DNA or RNA/ RNA duplexes. This PNA probe assay can be applied as a magnetically guidable tool for detection of DNA/RNA samples under different conditions." . "Nguyen, Hoai Viet" . . "Paramagnetic Particles and PNA Probe for Automated Separation and Electrochemical Detection of Influenza" . "RIV/00216305:26620/14:PU112580!RIV15-MSM-26620___" . . "0009-5893" . . . . "Considerable efforts have been devoted to the development of rapid and sensitive methods allowing the detection of viral nucleic acid. We herein describe an assay for identification of a specific influenza sequence. The suggested method was based on isolation using paramagnetic particles coupled with electrochemical detection of isolated product. Peptide nucleic acid (PNA) was used as a probe for hybridization and identification of the influenza-derived specific sequence. The use of PNA can show numerous benefits: PNA probe is not degradable by enzymes and the duplex of PNA with RNA/DNA is more thermostable and more resistant to pH changes than DNA/DNA or RNA/ RNA duplexes. This PNA probe assay can be applied as a magnetically guidable tool for detection of DNA/RNA samples under different conditions."@en . "6"^^ . "Nguyen, Hoai Viet" . . . "Paramagnetic Particles and PNA Probe for Automated Separation and Electrochemical Detection of Influenza"@en . "Krej\u010Dov\u00E1, Ludmila" . "DE - Spolkov\u00E1 republika N\u011Bmecko" . . "6"^^ . . "O, P(ED1.1.00/02.0068)" . "[5E65FAA1A0F5]" . . . . "Hynek, David" . . "8"^^ . "Paramagnetic Particles and PNA Probe for Automated Separation and Electrochemical Detection of Influenza" . . "Adam, Vojt\u011Bch" . . "Protein nucleic acid, Biosensor, Magneticparticle- based separation, Voltammetry"@en . . . . "Paramagnetic Particles and PNA Probe for Automated Separation and Electrochemical Detection of Influenza"@en . .