"326413" . "0173-0835" . "4"^^ . "Miniaturized electrochemical detector as a tool for detection of DNA amplified by PCR" . . "DE - Spolkov\u00E1 republika N\u011Bmecko" . "Electrophoresis" . . "Miniaturized electrochemical detector as a tool for detection of DNA amplified by PCR"@en . "2008 (29)" . "Kizek, Ren\u00E9" . "Adam, Vojt\u011Bch" . "Miniaturized electrochemical detector as a tool for detection of DNA amplified by PCR" . "Hub\u00E1lek, Jarom\u00EDr" . . "Carbon nanotubes, DNA, Gel electrophoresis on chip, PCR, Voltammetry"@en . "RIV/00216305:26220/09:PU85269!RIV10-AV0-26220___" . "H\u00FAska, Dalibor" . . . . . . "8"^^ . "This paper reports on the analysis of specific sequence of Phage Lambda DNA amplified by PCR. Agarose gel electrophoresis, gel electrophoresis on chip and stationary electrochemical instrument were employed for detection of amplicons obtained after 2, 4, 6, 8, 10, 15, 20, 25, 30 and 35 cycles. In the case of agarose gel electrophoresis the lowest detectable amount of DNA was obtained after 15 PCR cycles. Gel electrophoresis on chip offers higher sensitivity because the lowest detectable amount of amplicons by this technique was obtained after eight PCR cycles. Further we employed square wave voltammetry and various working electrodes (hanging mercury drop electrode, screen-printed carbon electrode and carbon-nanotube-based screen-printed electrodes) to detect amplicons. Amplicons obtained even after two cycles were detectable at all electrodes. To improve the selectivity of electrochemical detection carbon nanoelectrodes were off-line coupled with gel electrophoresis. Into the agarose gel the electrod"@en . . . . . . . "This paper reports on the analysis of specific sequence of Phage Lambda DNA amplified by PCR. Agarose gel electrophoresis, gel electrophoresis on chip and stationary electrochemical instrument were employed for detection of amplicons obtained after 2, 4, 6, 8, 10, 15, 20, 25, 30 and 35 cycles. In the case of agarose gel electrophoresis the lowest detectable amount of DNA was obtained after 15 PCR cycles. Gel electrophoresis on chip offers higher sensitivity because the lowest detectable amount of amplicons by this technique was obtained after eight PCR cycles. Further we employed square wave voltammetry and various working electrodes (hanging mercury drop electrode, screen-printed carbon electrode and carbon-nanotube-based screen-printed electrodes) to detect amplicons. Amplicons obtained even after two cycles were detectable at all electrodes. To improve the selectivity of electrochemical detection carbon nanoelectrodes were off-line coupled with gel electrophoresis. Into the agarose gel the electrod" . . . "24" . . "Miniaturized electrochemical detector as a tool for detection of DNA amplified by PCR"@en . "P(KAN208130801)" . "[5E99A23BB1EB]" . "RIV/00216305:26220/09:PU85269" . "1"^^ . "26220" .