"Vliv prost\u0159ed\u00ED vodn\u00EDch par na biologick\u00E9 vzorky pozorovan\u00E9 v EREM"@cs . "Environmental scanning electron microscope (ESEM) allows the visualisation of specimens that are difficult or impossible to image in a conventional high vacuum SEM. Pressure in the specimen chamber ranges from 1 Pa over 2000 Pa, which permits to observe specimens of beeing free from charging artefacts and containing different volume of water. But there are some difficulties with the observation of highly wet biological specimens in their natural state because during a pre-pumping process of the specimenn chamber an escape of water from the specimen occurs and thus its surface is damaged. The aim of this contribution is to find a method making possible to decrease water escape from the specimen during the initiation pumping process of the ESEM, immediately after the inserting of the specimen into the specimen chamber and during an image recording."@en . "biological specimens, imaging, EREM"@en . . "Neuveden" . "Influence of water environment on biological specimens imaging in ESEM"@en . . "Vliv prost\u0159ed\u00ED vodn\u00EDch par na biologick\u00E9 vzorky pozorovan\u00E9 v EREM"@cs . . . . "Influence of water environment on biological specimens imaging in ESEM" . "2"^^ . "610424" . "Environmental scanning electron microscope (ESEM) allows the visualisation of specimens that are difficult or impossible to image in a conventional high vacuum SEM. Pressure in the specimen chamber ranges from 1 Pa over 2000 Pa, which permits to observe specimens of beeing free from charging artefacts and containing different volume of water. But there are some difficulties with the observation of highly wet biological specimens in their natural state because during a pre-pumping process of the specimenn chamber an escape of water from the specimen occurs and thus its surface is damaged. The aim of this contribution is to find a method making possible to decrease water escape from the specimen during the initiation pumping process of the ESEM, immediately after the inserting of the specimen into the specimen chamber and during an image recording." . "RIV/00216305:26220/03:PU36425" . . . "Environment\u00E1ln\u00ED rastrovac\u00ED elektronov\u00FD mikroskop (EREM) umo\u017E\u0148uje pozorovat vzorky, kter\u00E9 je obt\u00ED\u017En\u00E9 \u010Di nemo\u017En\u00E9 pozorovat v REM. Tlak v komo\u0159e vzorku v rozsahu od 1Pa do 2000Pa dovoluje pozorovat izola\u010Dn\u00ED vzorky bez v\u00FDskytu n\u00E1bojov\u00FDch artefakt\u016F na jejich povrchu a vzorky obsahuj\u00EDc\u00ED vodu. Nicm\u00E9n\u011B ur\u010Dit\u00E9 probl\u00E9my p\u0159etrv\u00E1vaj\u00ED p\u0159i pozorov\u00E1n\u00ED vysoce vlhk\u00FDch biologick\u00FDch vzork\u016F v jejich p\u0159irozen\u00E9 podob\u011B a to p\u0159edev\u0161\u00EDm v pr\u016Fv\u011Bhu prvotn\u00EDch f\u00E1z\u00ED \u010Derp\u00E1n\u00ED mikroskopu, kdy doch\u00E1z\u00ED k odpa\u0159ov\u00E1n\u00ED vody ze vzork\u016F a jejichh po\u0161kozen\u00ED. C\u00EDlem t\u00E9to pr\u00E1ce je naj\u00EDt a vytvo\u0159it takov\u00E9 metody, umo\u017E\u0148uj\u00EDc\u00ED sn\u00EDzit odpar vody ze vzorku v EREM a zachovat tak jeho p\u0159irozenou podobu."@cs . "Pula" . "Influence of water environment on biological specimens imaging in ESEM" . "Ned\u011Bla, Vil\u00E9m" . "Pula" . . . "2"^^ . . "2003-06-01+02:00"^^ . "3"^^ . "Autrata, Rudolf" . . "Influence of water environment on biological specimens imaging in ESEM"@en . "P(GA102/01/1271), Z(MSM 262200010)" . . "Proceedings 6th Multinational Congress on Microscopy" . . . "RIV/00216305:26220/03:PU36425!RIV06-GA0-26220___" . . . "497-498" . "26220" . . "[F10EF64604EA]" . "Hork\u00FD, Drahom\u00EDr" . . .