"Boyde, Alan" . . . "Zikmund, Tom\u00E1\u0161" . . . . "Correlation between 3D imaging methods in studying bone architecture: SEM, microCT and confocal LM" . . "[ED72F99AC9C0]" . "Correlation between 3D imaging methods in studying bone architecture: SEM, microCT and confocal LM"@en . "Kvasnica, Luk\u00E1\u0161" . . . "Computerised x-ray microtomography (microCT) is increasingly used in the 3D study of bone microarchitecture and in quantifying bone volume fractions. However, the volumetric resolution in labora- tory apparatus for small rodent studies is at best several microns linear and recognisable detail characterising forming, resting and resorbing surfaces is completely missing. Backscattered electron mode scanning electron microscopy (BSE SEM) of both macerated 3D samples and polished surfaces of blocks of PMMA embedded tissue provides this information but samples have to be cut and processed. The same PMMA material is good for confocal fluores- cence microscopy (CSLM) for both tissue morphology and the study of tetracycline and calcein mineralising front labels to 50\u2013 200 microns deep to the block surface. With the recent acquisi- tion of SEM with variable chamber pressure to permit examina- tion of uncoated specimens, we are able to conduct CSLM after SEM for correlation studies. Here, we report new approa" . . "128856" . . "RIV/00216305:26210/12:PU99555" . "26210" . . "Correlation between 3D imaging methods in studying bone architecture: SEM, microCT and confocal LM"@en . . "2"^^ . . "3"^^ . "I" . . . "x-ray microtomography,scanning electron microscopy,confocal fluorescence microscopy,rat femur"@en . . "RIV/00216305:26210/12:PU99555!RIV13-MSM-26210___" . "Correlation between 3D imaging methods in studying bone architecture: SEM, microCT and confocal LM" . . "Computerised x-ray microtomography (microCT) is increasingly used in the 3D study of bone microarchitecture and in quantifying bone volume fractions. However, the volumetric resolution in labora- tory apparatus for small rodent studies is at best several microns linear and recognisable detail characterising forming, resting and resorbing surfaces is completely missing. Backscattered electron mode scanning electron microscopy (BSE SEM) of both macerated 3D samples and polished surfaces of blocks of PMMA embedded tissue provides this information but samples have to be cut and processed. The same PMMA material is good for confocal fluores- cence microscopy (CSLM) for both tissue morphology and the study of tetracycline and calcein mineralising front labels to 50\u2013 200 microns deep to the block surface. With the recent acquisi- tion of SEM with variable chamber pressure to permit examina- tion of uncoated specimens, we are able to conduct CSLM after SEM for correlation studies. Here, we report new approa"@en .