. "[63D342BAD67E]" . "RIV/00216305:26210/11:PU96865" . . . . "222039" . "Proch\u00E1zka, David" . "3"^^ . . "The question of biofilm definition has been debated in many discussions. We favour the simple definition from a recently published review \u2013 a biofilm is a thin coating comprised of living material. We report on chemical analysis of biofilms (algal and bacterial) and single microorganism cells. We combine the laser based analytical techniques \u2013 Laser Induced Breakdown Spectroscopy (LIBS), femtosecond Laser Ablation Inductively Coupled Plasma Time of Flight Mass Spectrometry (fs-LA-ICP-TOF-MS) and Raman spectroscopy/Raman tweezers to assess both the elemental composition and the character of the organic matter comprising the biofilms. We correlate the information obtained in Raman experiments with the data from the LIBS and TOF-MS experiments to obtain a complex set of data characterizing the biofilms cultured in different conditions (e.g. different culture media, metal-ion binding properties, time of cultivation, temperature etc.). These findings contribute to elucidating of the fundamental" . "P(ME09015), P(ME10061)" . . . "26210" . "The question of biofilm definition has been debated in many discussions. We favour the simple definition from a recently published review \u2013 a biofilm is a thin coating comprised of living material. We report on chemical analysis of biofilms (algal and bacterial) and single microorganism cells. We combine the laser based analytical techniques \u2013 Laser Induced Breakdown Spectroscopy (LIBS), femtosecond Laser Ablation Inductively Coupled Plasma Time of Flight Mass Spectrometry (fs-LA-ICP-TOF-MS) and Raman spectroscopy/Raman tweezers to assess both the elemental composition and the character of the organic matter comprising the biofilms. We correlate the information obtained in Raman experiments with the data from the LIBS and TOF-MS experiments to obtain a complex set of data characterizing the biofilms cultured in different conditions (e.g. different culture media, metal-ion binding properties, time of cultivation, temperature etc.). These findings contribute to elucidating of the fundamental"@en . "The Potential of Laser Based Analytical Techniques for the Characterization of Biofilms."@en . . "The Potential of Laser Based Analytical Techniques for the Characterization of Biofilms." . "Kaiser, Jozef" . . "RIV/00216305:26210/11:PU96865!RIV12-MSM-26210___" . . "The Potential of Laser Based Analytical Techniques for the Characterization of Biofilms." . . . "The Potential of Laser Based Analytical Techniques for the Characterization of Biofilms."@en . . "LIBS, biofilm, laser-ablation"@en . . "8"^^ . . . . . "Malina, Radom\u00EDr" . "http://www.emslibs2011.org/" . .