. "46" . "10812" . "Mysl\u00EDkov\u00E1, Kate\u0159ina" . . "000335009600003" . "Andrologia" . "Determination of retinol and alpha-tocopherol in human seminal plasma using an HPLC with UV detection"@en . "5" . . . "DE - Spolkov\u00E1 republika N\u011Bmecko" . . "RIV/00216275:25310/14:39898252" . "[C7AA106F1BFA]" . "7"^^ . "Hampl, Radek" . "10.1111/and.12103" . "tocopherol; seminal plasma; retinol; high-performance liquid chromatography; Butylated hydroxytoluene"@en . . . "Kan\u010F\u00E1r, Roman" . "Determination of retinol and alpha-tocopherol in human seminal plasma using an HPLC with UV detection"@en . . . . "25310" . . "I" . . "Determination of retinol and alpha-tocopherol in human seminal plasma using an HPLC with UV detection" . . . . . "Oxidative stress has been proposed as one of the potential causes for infertility in men. Retinol and alpha-tocopherol have an important role in the spermatozoa defences against oxidative stress. A method is described here for the simultaneous determination of retinol and alpha-tocopherol in human seminal plasma with a suitable sample preparation procedure to prevent retinol and alpha-tocopherol degradation. After adequate sample preparation, the samples were determined by reversed-phase column chromatography with UV detection. The analytical performance of this method was satisfactory. The intra-assay and inter-assay coefficients of variation were below 10%. The recoveries were as follows: 90.7% (CV 8.1%) for retinol and 98.2% (CV 4.8%) for alpha-tocopherol. No significant differences in both retinol and alpha-tocopherol concentration between the smokers and nonsmokers (15 +/- 7nm and 1.86 +/- 0.29 mu m versus 15 +/- 6nm and 1.93 +/- 0.45 mu m) were found. A selective high-performance liquid chromatography method for the determination of retinol and alpha-tocopherol in human seminal plasma was developed."@en . "Dr\u00E1bkov\u00E1, Petra" . "4"^^ . "3"^^ . "Determination of retinol and alpha-tocopherol in human seminal plasma using an HPLC with UV detection" . . "0303-4569" . "Oxidative stress has been proposed as one of the potential causes for infertility in men. Retinol and alpha-tocopherol have an important role in the spermatozoa defences against oxidative stress. A method is described here for the simultaneous determination of retinol and alpha-tocopherol in human seminal plasma with a suitable sample preparation procedure to prevent retinol and alpha-tocopherol degradation. After adequate sample preparation, the samples were determined by reversed-phase column chromatography with UV detection. The analytical performance of this method was satisfactory. The intra-assay and inter-assay coefficients of variation were below 10%. The recoveries were as follows: 90.7% (CV 8.1%) for retinol and 98.2% (CV 4.8%) for alpha-tocopherol. No significant differences in both retinol and alpha-tocopherol concentration between the smokers and nonsmokers (15 +/- 7nm and 1.86 +/- 0.29 mu m versus 15 +/- 6nm and 1.93 +/- 0.45 mu m) were found. A selective high-performance liquid chromatography method for the determination of retinol and alpha-tocopherol in human seminal plasma was developed." . "RIV/00216275:25310/14:39898252!RIV15-MSM-25310___" . .