"5"^^ . "H\u00E1jek, Tom\u00E1\u0161" . "Sta\u0148kov\u00E1, Magda" . . "RIV/00216275:25310/12:39895677!RIV13-GA0-25310___" . "P(GAP206/12/0398), S" . "11"^^ . "In-line coupled comprehensive HILICxRP systems should offer larger selectivity differences and better two-dimensional orthogonality than coupled RPxRP systems. However, this may not apply for all systems. The HILIC selectivity depends on the mix of selective polar and non-polar interactions with the functional groups, but also with the matrix of polar columns and depends on the sample type. We synthesized a new polar monolithic sulfobetaine polymethacrylate capillary column with excellent efficiency for low-molecular compounds. When used in the first, HILIC dimension coupled to core-shell or monolithic RP columns in the second dimension, this column provides much improved ortogonality for two-dimensional separations of phenolic and flavonoid compounds, in comparison to silica-bonded Diol, Polyethylene glycol or Zwitterionic columns. We investigated the performance of 11 short 5 cm and 3 cm columns for fast (1-2 min) gradient second-dimension separations. Band broadening or distortion may occur in directly coupled comprehensive HILICxRP systems, due to strong solvent-strength differences between the mobile phases used in the first and in the second dimension. To suppress this effect, low fraction volumes were collected from a 0.5 mm I.D. capillary monolithic sulfobetaine column at the flow-rate of a few microliters per min, coupled in-line with various core-shell columns operated at the maximum flow-rate. This setup with simultaneous gradient elution in the HILIC and in the RP dimension provided successful separation of natural antioxidants."@en . . "Optimization of Comprehensive Two-Dimensional Gradient Chromatography Coupling In-Line Hydrophilic Interaction and Reversed Phase Liquid Chromatography" . . "156940" . . . "Optimization of Comprehensive Two-Dimensional Gradient Chromatography Coupling In-Line Hydrophilic Interaction and Reversed Phase Liquid Chromatography" . . . "Optimization of Comprehensive Two-Dimensional Gradient Chromatography Coupling In-Line Hydrophilic Interaction and Reversed Phase Liquid Chromatography"@en . . "0021-9673" . "000312177300012" . "Optimization of Comprehensive Two-Dimensional Gradient Chromatography Coupling In-Line Hydrophilic Interaction and Reversed Phase Liquid Chromatography"@en . . . . . . "10.1016/j.chroma.2012.10.041" . . "In-line coupled comprehensive HILICxRP systems should offer larger selectivity differences and better two-dimensional orthogonality than coupled RPxRP systems. However, this may not apply for all systems. The HILIC selectivity depends on the mix of selective polar and non-polar interactions with the functional groups, but also with the matrix of polar columns and depends on the sample type. We synthesized a new polar monolithic sulfobetaine polymethacrylate capillary column with excellent efficiency for low-molecular compounds. When used in the first, HILIC dimension coupled to core-shell or monolithic RP columns in the second dimension, this column provides much improved ortogonality for two-dimensional separations of phenolic and flavonoid compounds, in comparison to silica-bonded Diol, Polyethylene glycol or Zwitterionic columns. We investigated the performance of 11 short 5 cm and 3 cm columns for fast (1-2 min) gradient second-dimension separations. Band broadening or distortion may occur in directly coupled comprehensive HILICxRP systems, due to strong solvent-strength differences between the mobile phases used in the first and in the second dimension. To suppress this effect, low fraction volumes were collected from a 0.5 mm I.D. capillary monolithic sulfobetaine column at the flow-rate of a few microliters per min, coupled in-line with various core-shell columns operated at the maximum flow-rate. This setup with simultaneous gradient elution in the HILIC and in the RP dimension provided successful separation of natural antioxidants." . "Vy\u0148uchalov\u00E1, Kate\u0159ina" . "\u010Cesla, Petr" . "1268" . . "RIV/00216275:25310/12:39895677" . . . "Journal of Chromatography A" . . "Jandera, Pavel" . "December" . "5"^^ . . . . "NL - Nizozemsko" . "Phenolic compounds.; Gradient elution; HILIC; Two-dimensional liquid chromatography"@en . "[CE662D441BF8]" . "25310" . .