. . . "A New Biosensor for Glucose Based on Carbon Paste and Enzyme Immobilized onto the Polyaniline Film"@en . . "10"^^ . . "A New Biosensor for Glucose Based on Carbon Paste and Enzyme Immobilized onto the Polyaniline Film" . . "Conductive polymer polyaniline (PANI) was electrochemically synthesized by controlled potential cycling as a film at the surface of a carbon paste electrode (CPE) from acidic aqueous solution containing 1 M HCl and 0.25 M aniline. The characterization of the PANI-CPE was carried out with the aid of cyclic voltammetry in 1 M HCl, obtaining three couple of peaks from which the two pairs were distinct, having proved the electroactivity of the PANI film. Then, the PANI-CPE system was for the first time used and tested as glucose biosensor, when the respective enzyme was potentiostatically immobilized onto the surface from 0.1 M phosphate buffer (pH 7.5) with 0.75 mg/mL GOD. Optimal conditions for the enzyme immobilization were investigated and the biosensor tested for glucose sensing within the concentration range of 1-500 mg/L D-glucose. All preliminary assays with the GOD/PANI-CPE configuration have shown favorable operational features of this new carbon paste biosensor whose further development and adaptation for flowing streams are also outlined."@en . "CS - St\u00E1tn\u00ED svaz Srbska a \u010Cern\u00E9 Hory" . . . "A New Biosensor for Glucose Based on Carbon Paste and Enzyme Immobilized onto the Polyaniline Film"@en . "P(LC06035), Z(MSM0021627502)" . "4"^^ . . "\u0160vancara, Ivan" . . "A New Biosensor for Glucose Based on Carbon Paste and Enzyme Immobilized onto the Polyaniline Film" . "000292330700014" . "6" . "3"^^ . "Conductive polymer polyaniline (PANI) was electrochemically synthesized by controlled potential cycling as a film at the surface of a carbon paste electrode (CPE) from acidic aqueous solution containing 1 M HCl and 0.25 M aniline. The characterization of the PANI-CPE was carried out with the aid of cyclic voltammetry in 1 M HCl, obtaining three couple of peaks from which the two pairs were distinct, having proved the electroactivity of the PANI film. Then, the PANI-CPE system was for the first time used and tested as glucose biosensor, when the respective enzyme was potentiostatically immobilized onto the surface from 0.1 M phosphate buffer (pH 7.5) with 0.75 mg/mL GOD. Optimal conditions for the enzyme immobilization were investigated and the biosensor tested for glucose sensing within the concentration range of 1-500 mg/L D-glucose. All preliminary assays with the GOD/PANI-CPE configuration have shown favorable operational features of this new carbon paste biosensor whose further development and adaptation for flowing streams are also outlined." . "Glucose; Glucose oxidase; Polyaniline film; Carbon paste; Biosensor"@en . "1452-3981" . . . . "Vyt\u0159as, Karel" . "RIV/00216275:25310/11:39892617" . "Sto\u010Des, Mat\u011Bj" . . "International Journal of Electrochemical Science" . "[3F69E73D32CF]" . "25310" . "Kalcher, Kurt" . . "183934" . . . . . "6" . . . "RIV/00216275:25310/11:39892617!RIV12-MSM-25310___" . .