. "Effect of Selected Fungicides on Fusarium Growth and toxins production" . "Effect of Selected Fungicides on Fusarium Growth and toxins production"@en . "SI" . . "Hrubo\u0161ov\u00E1, Dana" . . "CZ - \u010Cesk\u00E1 republika" . . . "RIV/00216275:25310/11:39892302" . . . . . . "Vyt\u0159asov\u00E1, Jarmila" . "PCR; fungicide; T-2 toxin; deoxynivalenol; mycotoxin; genus Fusarium"@en . . . . "We examine the effects of selected fungicides on the Fusarium growth and toxins production especially of DON and T-2 toxins. Appropriate nutritive media were prepared containing different concentrations of the fungicides tested , inoculated with Fusarium moulds, and incubated under various conditions. The extraction of Fusarium toxins and their determination were carried out after the incubation using Agra Quant(R)Deoxynivalenol Test kit and Agra Quant(R) T-2 toxin Test kit. The results indicated a considerable variability of the individual strains in the formation of toxins. The strains of Fusarium graminearum CCM F-683 and Fusarium spp. produced large quantities of both DON and T-2 toxins. Fusarium poae CCM F-584 and Fusarium sporotrichioides CCM F-352 always produced larger quantities of only T-2 toxin in the given nutrient media. The experiment showed that the use of lower concentrations of fungicides resulted in an increased production of the monitored mycotoxins. The presence of Tri5 gene in the tested Fusarium strains was confirmed by PCR. The enzyme - trichodiene synthase - catalyses the isomerisation and cyclisation of farnesyl phosphate to trichodiene, the first step in the biosynthetic pathway of trichothecenes."@en . "000299721700011" . "25310" . "Czech journal of food sciences (Potravin\u00E1\u0159sk\u00E9 v\u011Bdy)" . "29" . . "3"^^ . . "Effect of Selected Fungicides on Fusarium Growth and toxins production" . "Effect of Selected Fungicides on Fusarium Growth and toxins production"@en . "7"^^ . . . "1212-1800" . "S, Z(MSM0021627502)" . "3"^^ . "196443" . "We examine the effects of selected fungicides on the Fusarium growth and toxins production especially of DON and T-2 toxins. Appropriate nutritive media were prepared containing different concentrations of the fungicides tested , inoculated with Fusarium moulds, and incubated under various conditions. The extraction of Fusarium toxins and their determination were carried out after the incubation using Agra Quant(R)Deoxynivalenol Test kit and Agra Quant(R) T-2 toxin Test kit. The results indicated a considerable variability of the individual strains in the formation of toxins. The strains of Fusarium graminearum CCM F-683 and Fusarium spp. produced large quantities of both DON and T-2 toxins. Fusarium poae CCM F-584 and Fusarium sporotrichioides CCM F-352 always produced larger quantities of only T-2 toxin in the given nutrient media. The experiment showed that the use of lower concentrations of fungicides resulted in an increased production of the monitored mycotoxins. The presence of Tri5 gene in the tested Fusarium strains was confirmed by PCR. The enzyme - trichodiene synthase - catalyses the isomerisation and cyclisation of farnesyl phosphate to trichodiene, the first step in the biosynthetic pathway of trichothecenes." . "Mo\u0165kov\u00E1, Petra" . . "RIV/00216275:25310/11:39892302!RIV12-MSM-25310___" . "[19424C14E663]" . . . . .