"5"^^ . "CZ - \u010Cesk\u00E1 republika" . "Kan\u010F\u00E1r, Roman" . "18" . "\u017D\u00E1kov\u00E1, Pavla" . "S" . "Klinick\u00E1 biochemie a metabolismus" . "Kova\u0159\u00EDk, Jakub" . "Skalick\u00FD, Ji\u0159\u00ED" . . . . "2"^^ . "8"^^ . "RIV/00216275:25310/10:39881901!RIV11-MSM-25310___" . "Vzorky s\u00E9r byly deproteinov\u00E1ny pomoc\u00ED kyseliny chlorist\u00E9 a supernatanty byly analyzov\u00E1ny vysoce \u00FA\u010Dinnou kapalinovou chromatografi\u00ED na reverzn\u00ED f\u00E1zi. Pro separaci byla u\u017Eita kolona MAG 1, 4,6 x 150 mm, Biospher PSI 200, 5 um. Mobiln\u00ED f\u00E1z\u00ED byla sm\u011Bs 5% methanolu v 25 mmol/l dihydrogenfosfore\u010Dnanu sodn\u00E9ho (v/v), pH 4,75. Analytick\u00E9 parametry metody jsou vyhovuj\u00EDc\u00ED: varia\u010Dn\u00ED koeficienty p\u0159esnosti v s\u00E9rii a mezi s\u00E9riemi se pohybovaly pod 5 %. V\u00FDt\u011B\u017Enost, ur\u010Den\u00E1 metodou standardn\u00EDch p\u0159\u00EDdavk\u016F, se pohybovala v rozmez\u00ED 90,0-103,4 %. Mez stanovitelnosti byla 10,0 umol/l (3,3 pmol/n\u00E1st\u0159ik). V\u00FDsledky z\u00EDskan\u00E9 chromatografickou metodou dob\u0159e korelovaly s v\u00FDsledky z\u00EDskan\u00FDmi rutinn\u00ED enzymatickou metodou, av\u0161ak chromatografick\u00E1 metoda poskytovala vy\u0161\u0161\u00ED hodnoty, a to v z\u00E1vislosti na dan\u00E9 skupin\u011B pacient\u016F. zde prezentovan\u00E1 metoda je vhodn\u00E1 pro anal\u00FDzu t\u011Bch vzork\u016F, u nich\u017E klasick\u00E1 enzymatick\u00E1 metoda poskytuje nespolehliv\u00E9 v\u00FDsledky." . "3" . "Stanoven\u00ED kyseliny mo\u010Dov\u00E9 v lidsk\u00E9m s\u00E9ru pomoc\u00ED HPLC s UV detekc\u00ED - porovn\u00E1n\u00ED s enzymatickou metodou"@cs . "[BFD0DB85FE42]" . . "25310" . "Stanoven\u00ED kyseliny mo\u010Dov\u00E9 v lidsk\u00E9m s\u00E9ru pomoc\u00ED HPLC s UV detekc\u00ED - porovn\u00E1n\u00ED s enzymatickou metodou" . "1210-7921" . "Serum samples were deproteinized with perchloric acid and analyzed be reversed-phase high-performance liquid chromatography. For the separation, a reverse phase column MAG 1, 4.6 x 150 mm, Biospher PSI 200 C18, 5 um, was used. The mobile phase consisted of 5 % methanol in 25-mmol/L sodium dihydrogenphosphate (v/v), pH 4.75. The nalytical performance of this method is satisfactory: the intra-assay and inter-assay coefficients of variation were below 5 %. Quantitative recoveries of spiked serum samples were between 90.0-103.4 %. The limit of quantification was 10.0 umol/L (3.3 pmol/inject). Results obtained by chromatographic method correlated well with an enzymatic method, but gave at average higher values depending on a group of patients. Presented method is useful for the analysis of samples where the classical enzymatic method do not give reliable results."@en . . "Mocov\u00E1, Tereza" . . "Determination of uric acid in human serum usind HPLC with UV detection"@en . "a comparison of methods; enzymatic method; HPLC with UV detection; uric acid"@en . . "Determination of uric acid in human serum usind HPLC with UV detection"@en . . "RIV/00216275:25310/10:39881901" . "Stanoven\u00ED kyseliny mo\u010Dov\u00E9 v lidsk\u00E9m s\u00E9ru pomoc\u00ED HPLC s UV detekc\u00ED - porovn\u00E1n\u00ED s enzymatickou metodou"@cs . "289718" . . . "Vzorky s\u00E9r byly deproteinov\u00E1ny pomoc\u00ED kyseliny chlorist\u00E9 a supernatanty byly analyzov\u00E1ny vysoce \u00FA\u010Dinnou kapalinovou chromatografi\u00ED na reverzn\u00ED f\u00E1zi. Pro separaci byla u\u017Eita kolona MAG 1, 4,6 x 150 mm, Biospher PSI 200, 5 um. Mobiln\u00ED f\u00E1z\u00ED byla sm\u011Bs 5% methanolu v 25 mmol/l dihydrogenfosfore\u010Dnanu sodn\u00E9ho (v/v), pH 4,75. Analytick\u00E9 parametry metody jsou vyhovuj\u00EDc\u00ED: varia\u010Dn\u00ED koeficienty p\u0159esnosti v s\u00E9rii a mezi s\u00E9riemi se pohybovaly pod 5 %. V\u00FDt\u011B\u017Enost, ur\u010Den\u00E1 metodou standardn\u00EDch p\u0159\u00EDdavk\u016F, se pohybovala v rozmez\u00ED 90,0-103,4 %. Mez stanovitelnosti byla 10,0 umol/l (3,3 pmol/n\u00E1st\u0159ik). V\u00FDsledky z\u00EDskan\u00E9 chromatografickou metodou dob\u0159e korelovaly s v\u00FDsledky z\u00EDskan\u00FDmi rutinn\u00ED enzymatickou metodou, av\u0161ak chromatografick\u00E1 metoda poskytovala vy\u0161\u0161\u00ED hodnoty, a to v z\u00E1vislosti na dan\u00E9 skupin\u011B pacient\u016F. zde prezentovan\u00E1 metoda je vhodn\u00E1 pro anal\u00FDzu t\u011Bch vzork\u016F, u nich\u017E klasick\u00E1 enzymatick\u00E1 metoda poskytuje nespolehliv\u00E9 v\u00FDsledky."@cs . . . . . . "Stanoven\u00ED kyseliny mo\u010Dov\u00E9 v lidsk\u00E9m s\u00E9ru pomoc\u00ED HPLC s UV detekc\u00ED - porovn\u00E1n\u00ED s enzymatickou metodou" . .