"312831" . . "Hrube\u0161, Michal" . "P(LC06035), Z(MSM0021627502)" . "Vyt\u0159asov\u00E1, Jarmila" . . . "Electroimmunoassay for detection of bacterial cells" . "An amperometric method for Salmonella detection is presented as an example of electroimmunoassay of bacterial cells. The method is based on the reaction of salmonella with an enzyme-linked specific antibody (alkaline phosphatase), forming salmonella-antibody-alkaline phosphatase (SAAP) conjugates. After their hydrolysis, the products formed (phenols) are detected amperometrically using a carbon paste electrode; current signals were monitored at 0.65 V vs. Ag/AgCl/sat. KCl. A medium of pH = 10.00 was found as the best. Amount of phenol generated by SAAP was proportional to the number of Salmonella Enteritidis in a sample" . . "4"^^ . "Electroimmunoassay for detection of bacterial cells"@en . "978-981-283-754-7" . "788"^^ . . . "25310" . . "3"^^ . "World Scientific Publishing Co." . . . "alkaline phosphatase; amperometric detection; carbon paste electrode; Salmonella Enteritidis"@en . . . "Pe\u0161kov\u00E1, Iva" . . "\u010Cervenka, Libor" . . "5"^^ . . . "Vyt\u0159as, Karel" . . "An amperometric method for Salmonella detection is presented as an example of electroimmunoassay of bacterial cells. The method is based on the reaction of salmonella with an enzyme-linked specific antibody (alkaline phosphatase), forming salmonella-antibody-alkaline phosphatase (SAAP) conjugates. After their hydrolysis, the products formed (phenols) are detected amperometrically using a carbon paste electrode; current signals were monitored at 0.65 V vs. Ag/AgCl/sat. KCl. A medium of pH = 10.00 was found as the best. Amount of phenol generated by SAAP was proportional to the number of Salmonella Enteritidis in a sample"@en . "Electroimmunoassay for detection of bacterial cells"@en . . . "RIV/00216275:25310/09:00008382" . . "RIV/00216275:25310/09:00008382!RIV11-MSM-25310___" . "Current Research topics in Applied Microbiology and Microbial Biotechnology" . "Electroimmunoassay for detection of bacterial cells" . "[32E855A815D1]" . "Mountain View" . .