"Objectives: Streptococcus dysgalactiae ssp. equisimilis belongs to Lancefield serogroup G, which forms heterogeneous group of beta-hemolytic streptococci. Presently, three taxonomic types of this serological group can be distinguished, as follows: minute colony formers from humans (S. anginosus group), and large colony formers from humans (S. dysgalactiae ssp. equisimilis) and animals (S. canis). In humans, they may colonize pharynx, skin, gastrointestinal and female genital tract. In recent years, they have been reported with increasing frequency as a cause of variety of human infections, such as endocarditis, arthritis, pharyngitis and sepsis. The differentiation of streptococci traditionally relies on the evaluation of growth, serological and biochemical properties. However, developments in genetic technology have resulted in new methods that can be used for identification of bacteria. The aim of the present work was the development of new PCR assay for the rapid and reliable identification of S. d"@en . "PCR assay for detection of Streptococcus dysgalactiae ssp. equisimilis"@en . . "PCR assay for detection of Streptococcus dysgalactiae ssp. equisimilis" . "1"^^ . "PCR detekce Streptococcus dysgalactiae ssp. equisimilis"@cs . "Objectives: Streptococcus dysgalactiae ssp. equisimilis belongs to Lancefield serogroup G, which forms heterogeneous group of beta-hemolytic streptococci. Presently, three taxonomic types of this serological group can be distinguished, as follows: minute colony formers from humans (S. anginosus group), and large colony formers from humans (S. dysgalactiae ssp. equisimilis) and animals (S. canis). In humans, they may colonize pharynx, skin, gastrointestinal and female genital tract. In recent years, they have been reported with increasing frequency as a cause of variety of human infections, such as endocarditis, arthritis, pharyngitis and sepsis. The differentiation of streptococci traditionally relies on the evaluation of growth, serological and biochemical properties. However, developments in genetic technology have resulted in new methods that can be used for identification of bacteria. The aim of the present work was the development of new PCR assay for the rapid and reliable identification of S. d" . "Z(MSM0021627502)" . "Kroupa, Tom\u00E1\u0161" . "Vydr\u017Ealov\u00E1, Mark\u00E9ta" . "386115" . "2008-04-22+02:00"^^ . "PCR assay for detection of Streptococcus dysgalactiae ssp. equisimilis"@en . . . "Streptococcus dysgalactiae ssp. equisimilis pat\u0159\u00ED mezi streptokoky se skupinov\u00FDm antigenem G, kter\u00E9 tvo\u0159\u00ED heterogenn\u00ED skupinu beta-hemolytick\u00FDch streptokok\u016F. V sou\u010Dasnosti se do t\u00E9to serologick\u00E9 skupiny \u0159ad\u00ED t\u0159i taxony: Streptococcus dysgalactiae ssp. equisimilis, S. canis a skupina S. anginosus. Mohou vyskytovat na k\u016F\u017Ei a na sliznic\u00EDch nosohltanu, gastrointestin\u00E1ln\u00EDho a urogenit\u00E1ln\u00EDho traktu lid\u00ED. V posledn\u00ED dob\u011B jsou v\u0161ak st\u00E1le \u010Dast\u011Bji zji\u0161\u0165ov\u00E1ny jako z\u00E1va\u017En\u00E1 p\u0159\u00ED\u010Dina r\u016Fzn\u00FDch, p\u0159ev\u00E1\u017En\u011B akutn\u00EDch infekc\u00ED, jako jsou endokarditidy, artritidy, faryngitidy a sepse. V\u011Bt\u0161ina klinick\u00FDch laborato\u0159\u00ED neprov\u00E1d\u00ED druhovou typizaci streptokok\u016F, ale omezuje se pouze na posouzen\u00ED morfologie koloni\u00ED, biochemick\u00FDch vlastnost\u00ED a pr\u016Fkaz skupinov\u00E9ho antigenu. C\u00EDlem na\u0161\u00ED studie bylo vyvinout novou PCR metody, kter\u00E1 by umo\u017Enila rychlou a spolehlivou identifikaci S. dysgalactiae ssp. equisimilis."@cs . "[232802771D78]" . . . "group G streptococci; PCR; S. dysgalactiae ssp. equisimilis"@en . "Clinical Microbiology and Infection" . . "Lyskov\u00E1, Petra" . "Oxford" . . "1198-743X" . "PCR detekce Streptococcus dysgalactiae ssp. equisimilis"@cs . . "PCR assay for detection of Streptococcus dysgalactiae ssp. equisimilis" . . "4"^^ . "Barcelona, Spain" . . . "Blackwell Publishing Ltd" . "25310" . . . . "Mazurov\u00E1, Jaroslava" . . "4"^^ . . "RIV/00216275:25310/08:00007880" . . . "RIV/00216275:25310/08:00007880!RIV09-MSM-25310___" . .