"Elektrochemick\u00E1 detekce mikroorganism\u016F s vyu\u017Eit\u00EDm uhl\u00EDkov\u00FDch pastov\u00FDch elektrod"@cs . . "2"^^ . "P(GA203/08/1536), Z(MSM0021627502)" . "RIV/00216275:25310/08:00007638!RIV09-GA0-25310___" . . "University of Ljublana" . . "Detection of Microorganisms by Carbon Paste Electrode - The Study of Different Technique/Buffer/Substrate Combinations" . . . "Ljublana" . "25310" . . "978-961-6286-96-1" . . "Ljubljana, Slovenia" . . . . "RIV/00216275:25310/08:00007638" . "The principle of detection is based on enzymatic activity of alkaline phosphatase enzyme1 a component of enzyme-antibody-bacteria immunocomplex, which provides the change of enzymatic substrate (phenyl phosphate2, \u03B1 ? naphthyl phosphate3) to electrochemical active product (phenol, naphthol). It?s quantified in five different buffers (carbonate, TBS2, DEA3, B-R and phosphate buffer) by voltammetric techniques (linear sweep LSV, differential pulse DPV2, square wave SWV4, normal pulse NPV5, alternate current ACV and cyclic voltammetry CV6) and amperometric method (AD1). Three electrode system is used with Ag/AgCl/3M KCl reference electrode, platinum wire auxiliary electrode and bare carbon paste electrode2,3 with 0,2mm diameter as working electrode. No stripping analysis is used, no modification of carbon paste electrode as well." . "2"^^ . . . . "4"^^ . "electrochemical detection; immunocomplex; Salmonella; carbon paste electrode; antibodies"@en . "362822" . "[45DE3B2E2211]" . "Detection of Microorganisms by Carbon Paste Electrode - The Study of Different Technique/Buffer/Substrate Combinations" . "YISAC 2008: 15th Young Investigators' Seminar on Analytical Chemistry" . . . "Detection of Microorganisms by Carbon Paste Electrode - The Study of Different Technique/Buffer/Substrate Combinations"@en . "Elektrochemick\u00E1 detekce mikroorganism\u016F s vyu\u017Eit\u00EDm uhl\u00EDkov\u00FDch pastov\u00FDch elektrod"@cs . . "Klodov\u00E1, Kate\u0159ina" . "The principle of detection is based on enzymatic activity of alkaline phosphatase enzyme1 a component of enzyme-antibody-bacteria immunocomplex, which provides the change of enzymatic substrate (phenyl phosphate2, \u03B1 ? naphthyl phosphate3) to electrochemical active product (phenol, naphthol). It?s quantified in five different buffers (carbonate, TBS2, DEA3, B-R and phosphate buffer) by voltammetric techniques (linear sweep LSV, differential pulse DPV2, square wave SWV4, normal pulse NPV5, alternate current ACV and cyclic voltammetry CV6) and amperometric method (AD1). Three electrode system is used with Ag/AgCl/3M KCl reference electrode, platinum wire auxiliary electrode and bare carbon paste electrode2,3 with 0,2mm diameter as working electrode. No stripping analysis is used, no modification of carbon paste electrode as well."@en . . . . "C\u00EDlem pr\u00E1ce je navrhnout nejv\u00FDhodn\u011Bj\u0161\u00ED kombinaci pufru a enzymatick\u00E9ho substr\u00E1tu pro amperometrickou metodu (AD) stanoven\u00ED mikroorganism\u016F na z\u00E1klad\u011B detek\u010Dn\u00EDho limitu (LOD) a meze stanoven\u00ED (LOQ) pro jednoho ze z\u00E1stupc\u016F patogenn\u00EDch bakteri\u00ED v potravin\u00E1ch (Salmonella Enteritidis). Pro studii byly vybr\u00E1ny \u010Dty\u0159i r\u016Fzn\u00E9 pufry (uhli\u010Ditanov\u00FD pufr, TBS, DEA, B-R) a dva enzymatick\u00E9 substr\u00E1ty (fenylfosf\u00E1t, 1-naftylfosf\u00E1t). Princip detekce salmonely je zalo\u017Een na enzymatick\u00E9 aktivit\u011B enzymu alkalick\u00E9 fosfat\u00E1zy, kter\u00FD je sou\u010D\u00E1st\u00ED imunokomplexu slo\u017Een\u00E9ho z enzymu-protil\u00E1tky-bakterie. Enzym umo\u017E\u0148uje p\u0159em\u011Bnit enzymatick\u00FD substr\u00E1t na elektrochemicky aktivn\u00ED produkt (fenol, 1-naftol)."@cs . "2008-07-05+02:00"^^ . . "Vyt\u0159asov\u00E1, Jarmila" . "Vyt\u0159as, Karel" . "Hrube\u0161, Michal" . . "Detection of Microorganisms by Carbon Paste Electrode - The Study of Different Technique/Buffer/Substrate Combinations"@en .