"B\u00EDlkov\u00E1, Zuzana" . "RIV/00216275:25310/07:00006247!RIV08-MSM-25310___" . "V\u00EDde\u0148" . "Springer-Verlage. Wien" . . . . . "Hlavn\u00ED proteinovou sou\u010D\u00E1st\u00ED amyloidov\u00FDch plak\u016F nach\u00E1zen\u00FDch v mozc\u00EDch pacient\u016F s Alzheimerovou chorobou je peptid amyloid-\u03B2 (A\u03B2 or \u03B2A4). b-amyloidov\u00E9 peptidy (o molekulov\u00E9 hmotnosti kolem 4,5 kDa) maj\u00ED p\u0159ibli\u017En\u011B 42 aminokyselinov\u00FDch zbytk\u016F. Ukl\u00E1d\u00E1n\u00ED a agregace t\u011Bchto peptid\u016F je pova\u017Eov\u00E1na za d\u016Fle\u017Eit\u00FD patologick\u00FD jev pro vznik seniln\u00EDch plak\u016F. Hladiny peptid\u016F A\u03B2 a proteinu tau v mozkom\u00ED\u0161n\u00EDm moku a/nebo plasm\u011B spl\u0148uj\u00ED krit\u00E9ria pro spolehliv\u00E9 testov\u00E1n\u00ED intra vitam a pat\u0159\u00ED mezi biomarkery podporuj\u00EDc\u00ED diagnostiku Alzheimerovy choroby. B\u011B\u017En\u011B vyu\u017E\u00EDvan\u00E9 syst\u00E9my SDS-PAGE nejsou vhodn\u00E9 pro rozli\u0161en\u00ED A peptid\u016F li\u0161\u00EDc\u00EDch se pouze o jeden \u010Di dva aminokyselinov\u00E9 zbytky v d\u00E9lce peptidov\u00E9ho \u0159et\u011Bzce. Proto byly testov\u00E1ny i dal\u0161\u00ED elektroforetick\u00E9 techniky z hlediska jejich u\u017Eite\u010Dnosti pro separaci A peptid\u016F A\u03B2(1-38), A\u03B2(1-40) and A\u03B2(1-42). Z\u00E1kladn\u00EDm zvolen\u00FDm syst\u00E9mem byla Tris-Tricin SDS-PAGE, d\u00E1le SDS-PAGE s ureou a kyselinou octovou (HAc) jako denaturuj\u00EDc\u00EDmi \u010Dinidly. Pou\u017Eita byla t"@cs . . "1"^^ . "The main protein component of the amyloid deposits found in the brains of Alzheimer\u00B4s disease patients is a peptide termed amyloid-\u03B2 (A\u03B2 or \u03B2A4). The amyloid-\u03B2 peptides (molecular weight about 4,5 kDa) are approximately 42-amino acid residue peptides. Peptides deposition and aggregation are thought to be important pathological events in the formation of Alzheimer\u00B4s disease plaque. The concentrations of A\u03B2 peptides and tau protein(s) in cerebrospinal fluid and/or blood belong to the candidate biomarkers supporting diagnose of Alzheimer?s disease and they have been proven to fulfill the criteria for good diagnostic tests intra vitam. Conventional SDS-PAGE system is not suited for separating of amyloid-\u03B2 peptides A\u03B2(1-38), A\u03B2(1-40) and A\u03B2(1-42). Alternative electrophoretic techniques have been compared for their usefulness in separation analysis of peptides corresponding to amino acids (1-38), (1-40) and (1- 42) of the amyloid-\u03B2 sequence. The first sy"@en . . "RIV/00216275:25310/07:00006247" . "The main protein component of the amyloid deposits found in the brains of Alzheimer\u00B4s disease patients is a peptide termed amyloid-\u03B2 (A\u03B2 or \u03B2A4). The amyloid-\u03B2 peptides (molecular weight about 4,5 kDa) are approximately 42-amino acid residue peptides. Peptides deposition and aggregation are thought to be important pathological events in the formation of Alzheimer\u00B4s disease plaque. The concentrations of A\u03B2 peptides and tau protein(s) in cerebrospinal fluid and/or blood belong to the candidate biomarkers supporting diagnose of Alzheimer?s disease and they have been proven to fulfill the criteria for good diagnostic tests intra vitam. Conventional SDS-PAGE system is not suited for separating of amyloid-\u03B2 peptides A\u03B2(1-38), A\u03B2(1-40) and A\u03B2(1-42). Alternative electrophoretic techniques have been compared for their usefulness in separation analysis of peptides corresponding to amino acids (1-38), (1-40) and (1- 42) of the amyloid-\u03B2 sequence. The first sy" . "Electrophoretic separation of amyloid beta peptides"@en . "Electrophoretic separation of amyloid beta peptides"@en . "Zdra\u017Eilov\u00E1, Pavla" . "Elektroforetick\u00E1 separace beta amyloidov\u00FDch peptid\u016F"@cs . "Holub, Du\u0161an" . "P(LC06035), Z(MSM0021627502)" . "0939-4451" . . "[A5BBE8636A87]" . . "Elektroforetick\u00E1 separace beta amyloidov\u00FDch peptid\u016F"@cs . . "Amino Acids" . "Electrophoretic separation of amyloid beta peptides" . . . . . "25310" . . . "3"^^ . . "amyloid beta peptide; SDS-PAGE; Alzheimer\u00B4s disease"@en . . "Electrophoretic separation of amyloid beta peptides" . "2"^^ . "LII" . . "419791" .