"Optimizing of the Amperometric Method for Salmonella Enteritidis Detection Using Carbon Paste Electrode"@en . . "Optimalizace metody amperometrick\u00E9 detekce Salmonella Enteritidis s vyu\u017Eit\u00EDm uhl\u00EDkov\u00E9 pastov\u00E9 elektrody"@cs . "Optimizing of the Amperometric Method for Salmonella Enteritidis Detection Using Carbon Paste Electrode" . "Optimalizace metody amperometrick\u00E9 detekce Salmonella Enteritidis s vyu\u017Eit\u00EDm uhl\u00EDkov\u00E9 pastov\u00E9 elektrody"@cs . . "Pe\u0161kov\u00E1, Iva" . . "282"^^ . "Pardubice" . . . "Kristlov\u00E1, Jana" . "RIV/00216275:25310/07:00006161!RIV08-MSM-25310___" . "Univerzita Pardubice" . "\u010Cervenka, Libor" . "RIV/00216275:25310/07:00006161" . "2"^^ . . "Vyt\u0159asov\u00E1, Jarmila" . "An amperometric method for salmonella detection was optimized. The method is based on the reaction of salmonella with an enzyme-linked specific antibody (linked by alcaline phosphatase), froming salmonella-antibody-alkaline phosphatase (SAAP) conjugates. After substrate hydrolysis, the product formed (phenol) are detected amperometrically using a carbon paste electrode; current signals were monited at 0.65 V vs. Ag/AgCl/sat. KCl. A medium of pH 10 was found as the best. Amount of phenol generated bz SAAP conjugate was proportional to the number of Salmonella enteritidis in a sample"@en . . . . "[667A4D522817]" . . . "YISAC 07 Proceedings" . "5"^^ . "An amperometric method for salmonella detection was optimized. The method is based on the reaction of salmonella with an enzyme-linked specific antibody (linked by alcaline phosphatase), froming salmonella-antibody-alkaline phosphatase (SAAP) conjugates. After substrate hydrolysis, the product formed (phenol) are detected amperometrically using a carbon paste electrode; current signals were monited at 0.65 V vs. Ag/AgCl/sat. KCl. A medium of pH 10 was found as the best. Amount of phenol generated bz SAAP conjugate was proportional to the number of Salmonella enteritidis in a sample" . "Salmonella enteritidis; carbon paste electrode; amperometric detection; alkaline phosphatase"@en . "Optimizing of the Amperometric Method for Salmonella Enteritidis Detection Using Carbon Paste Electrode"@en . . "978-80-7194-969-5" . "V r\u00E1mci v\u00FDzkumu byly optimalizov\u00E1ny podm\u00EDnky pro amperometrickou detekci bun\u011Bk Salmonella s vyu\u017Eit\u00EDm uhl\u00EDkov\u00E9 pastov\u00E9 elektrody. Metoda je zalo\u017Eena na reakci bun\u011Bk salmonely se specifickou protil\u00E1tkou zna\u010Dnou alkalickou fosfat\u00E1zou. Po hydrol\u00FDze substr\u00E1tu byl vznikl\u00FD fenol detekov\u00E1n amperometricky s vyu\u017Eit\u00EDm uhl\u00EDkov\u00E9 pastov\u00E9 elektrody. Sign\u00E1l proudu byl detekov\u00E1n p\u0159i 0.65 V vs. Ag/AgCl/sat. KCl. nejvhodn\u011Bj\u0161\u00EDm prosted\u00EDm pro detekci byl pufr s pH 10. Mno\u017Estv\u00ED fenolu odpov\u00EDd\u00E1 po\u010Dtu bun\u011Bk Salmonella Enteritidis ve vzorku."@cs . "25310" . . . "Hrube\u0161, Michal" . . "86-91" . . "2007-06-25+02:00"^^ . . "Pardubice, \u010Cesk\u00E1 republika" . "439934" . . "Optimizing of the Amperometric Method for Salmonella Enteritidis Detection Using Carbon Paste Electrode" . "P(LC06035), Z(MSM0021627502)" . . .