"4"^^ . "P(GA203/05/2106), Z(MSM0021627502)" . . "504083" . . "412" . . "25310" . "Vyt\u0159asov\u00E1, Jarmila" . . . "[36B94DA8D0F9]" . "International Committee on Food Microbiology and Hygiene" . "Toxinogenn\u00ED fus\u00E1ria v potravin\u00E1ch a krmivech."@cs . "Bologna" . . . "\u010Cervenka, Libor" . "Svobodov\u00E1, Lucie" . . . "Toxigenic Fusarium in Food and Feed"@en . "Toxinogenn\u00ED fus\u00E1ria v potravin\u00E1ch a krmivech."@cs . "20th International ICFMH Symposium Food Micro 2006" . . "2006-08-29+02:00"^^ . "Bro\u017Ekov\u00E1, Iveta" . . . "Pejchalov\u00E1, Marcela" . "Fusarium spp.; Fusarium graminearum; toxigenic fungi; polymerase chain reaction; Tri5 gene; trichothecenes; mycotoxins"@en . "RIV/00216275:25310/06:00004294!RIV08-MSM-25310___" . . "ur\u010Deno pro seznam publikac\u00ED"@cs . . "Toxigenic Fusarium in Food and Feed"@en . "RIV/00216275:25310/06:00004294" . "1"^^ . . "961-90346-3-5" . "Toxigenic Fusarium in Food and Feed" . "Bologna, It\u00E1lie" . . "Contamination of small-grain cereals with the fungal species Fusarium graminearum, F. culmorum, F. poae and F. sporotrichioides is an important source of trichothecenes, zearalenone and other mycotoxins which cause serious diseases in human and animals. Early detection and control of these Fusarium species is crucial to prevent toxins entering the food chain and a useful tool in disease management practices. Two PCR methods were evaluated for the successful identification of Fusarium species. The PCR was performed using a primer specifically targeted to Tri5 gene. The extraction technique of DNA by Cenis (1992) and the PCR procedure according to Edwards et al. (2001)) were compared with the DNA isolation and amplification using the Plant PCR Kit. The sensitivity of the latter method was determined using the reference strain of Fusarium graminearum CCM F-683 and the selectivity was examined using reference cultures of different genera of fungi naturally occurring in food and feedstuff. Fifty food an"@en . . . . "5"^^ . . . "Contamination of small-grain cereals with the fungal species Fusarium graminearum, F. culmorum, F. poae and F. sporotrichioides is an important source of trichothecenes, zearalenone and other mycotoxins which cause serious diseases in human and animals. Early detection and control of these Fusarium species is crucial to prevent toxins entering the food chain and a useful tool in disease management practices. Two PCR methods were evaluated for the successful identification of Fusarium species. The PCR was performed using a primer specifically targeted to Tri5 gene. The extraction technique of DNA by Cenis (1992) and the PCR procedure according to Edwards et al. (2001)) were compared with the DNA isolation and amplification using the Plant PCR Kit. The sensitivity of the latter method was determined using the reference strain of Fusarium graminearum CCM F-683 and the selectivity was examined using reference cultures of different genera of fungi naturally occurring in food and feedstuff. Fifty food an" . . "Toxigenic Fusarium in Food and Feed" . . . .