. "Sobodov\u00E1, Lucie" . . . "Two methods of isolation of DNA were evaluated for successful identification of Fusarium species. The PCR was performed using primer specifically targeted to Tri5 gene. Extraction technique of DNA by Cenis (1992) and PCR procedure according to Edwards et al. (2001) was compared with DNA isolation and amplification using Plant PCR Kit. Sensitivity of the latter method was determined using reference strain of Fusarium graminearum CCM F-683 and selectivity was examined using reference cultures of different genera of fungi naturally occurred in food and feedstuff. Morphological features are important for species classification whereby PCR can distinguish between toxigenic and non-toxigenic fungi of Fusarium. These fungi could be detected within 2 days, while their identification by macroscopic and microscopic features takes 2-3 weeks. Thus the PCR technique has proved itself as reliable and rapid method for detection of toxigenic species of Fusarium."@en . "517474" . "RIV/00216275:25310/05:00003162!RIV06-GA0-25310___" . "Detekce fus\u00E1ri\u00ED metodou PCR"@cs . . "Detekce fus\u00E1ri\u00ED metodou PCR"@cs . "\u010Cervenka, Libor" . "P(GA203/05/2106), Z(MSM0021627502)" . . "Two methods of isolation of DNA were evaluated for successful identification of Fusarium species. The PCR was performed using primer specifically targeted to Tri5 gene. Extraction technique of DNA by Cenis (1992) and PCR procedure according to Edwards et al. (2001) was compared with DNA isolation and amplification using Plant PCR Kit. Sensitivity of the latter method was determined using reference strain of Fusarium graminearum CCM F-683 and selectivity was examined using reference cultures of different genera of fungi naturally occurred in food and feedstuff. Morphological features are important for species classification whereby PCR can distinguish between toxigenic and non-toxigenic fungi of Fusarium. These fungi could be detected within 2 days, while their identification by macroscopic and microscopic features takes 2-3 weeks. Thus the PCR technique has proved itself as reliable and rapid method for detection of toxigenic species of Fusarium." . "Vyt\u0159asov\u00E1, Jarmila" . . "Detection of Fusarium using the PCR Method" . "2005-08-14+02:00"^^ . "Detection of Fusarium using the PCR Method"@en . "[A4D48B345C6F]" . "963-661-681-7" . . "417-423" . . "Detection of Fusarium using the PCR Method" . "2"^^ . "4"^^ . . . "Byly optimalizov\u00E1ny dv\u011B metody. Jednak postup extrakce DNA dle Cenise (1992) a postup PCR dle Edwardse et al. (2001), jednak byl pro izolaci DNA a jej\u00ED n\u00E1slednou amplifikaci pou\u017Eit komer\u010Dn\u00ED set ur\u010Den\u00FD pro izolaci a amplifikaci genomick\u00E9 DNA z list\u016F rostlin. Pou\u017Eit\u00ED komer\u010Dn\u00EDho setu se pro detekci toxinogenn\u00EDch pl\u00EDsn\u00ED rodu Fusarium jev\u00ED jako spolehliv\u011Bj\u0161\u00ED a rychlej\u0161\u00ED. Pro optimalizaci a stanoven\u00ED citlivosti reakce byla pou\u017Eita sb\u00EDrkov\u00E1 kultura F. graminearum CCM F - 683. Selektivita metody byla ov\u011B\u0159ena na sb\u00EDrkov\u00FDch kultur\u00E1ch pl\u00EDsn\u00ED, kter\u00E9 se mohou vyskytovat v re\u00E1ln\u00FDch vzorc\u00EDch potravin spole\u010Dn\u011B s pl\u00EDsn\u011Bmi rodu Fusarium.Polymerasov\u00E1 \u0159et\u011Bzov\u00E1 reakce byla pou\u017Eita pro sledov\u00E1n\u00ED p\u0159\u00EDtomnosti Tri5 genu k\u00F3duj\u00EDc\u00EDho tvorbu trichothecenov\u00FDch mykotoxin\u016F. Bylo vy\u0161et\u0159eno 50 vzork\u016F potravin a krmiv. Sedm z nich bylo pozitivn\u00EDch na p\u0159\u00EDtomnost pl\u00EDsn\u00ED rodu Fusarium. P\u011Bt druh\u016F rodu Fusarium vyizolovan\u00FDch ze vzork\u016F obsahovalo v DNA Tri5 gen. U jednotliv\u00FDch sb\u00EDrkov\u00FDch pl\u00EDsn\u00ED i pl\u00EDsn\u00ED z\u00EDskan\u00FDch ze vzork\u016F byly rovn\u011B\u017E sledov"@cs . "University of Miskolc" . "Detection of Fusarium using the PCR Method"@en . "RIV/00216275:25310/05:00003162" . . "University of Miskolc" . . . . . "Natural Sciences" . "7"^^ . . . "Miskolc Hungary" . "Fusarium graminearum; toxigenic fungi; PCR; trichothecenes"@en . "Zachov\u00E1, Iveta" . . . "25310" . .