"RIV/00216275:25310/05:00002796" . "Podivice, \u010Cesk\u00E1 Republika" . "[7D4DAC9E9EA4]" . . "Problematika stanoven\u00ED Km u enzymov\u00FDch reaktor\u016F"@cs . . . "Pardubice" . . "25310" . . . "Enzymov\u00E9 reaktory p\u0159edstavuj\u00ED typ biokatalyz\u00E1toru, kter\u00FD se od voln\u00FDch enzym\u016F li\u0161\u00ED z\u00E1kladn\u00EDmi kinetick\u00FDmi parametry. K ovlivn\u011Bn\u00ED kinetick\u00FDch konstant doch\u00E1z\u00ED ji\u017E na \u00FArovni imobilizace enzymu, a to volbou metody imobilizace, volbou pH pro vazbu enzymu a tak\u00E9 volbou nosi\u010De. U enzymov\u00FDch reaktor\u016F lze stanovit pouze tzv. %22zd\u00E1nlivou%22 konstantu Michaelis - Mentenov\u00E9 Km (Km,app). Je to konstanta vyjad\u0159uj\u00EDc\u00ED afinitu enzymu k dan\u00E9mu substr\u00E1tu zahrnuj\u00EDc\u00ED tak\u00E9 vliv mikrookol\u00ED nosi\u010De a dif\u016Fzn\u00EDch vliv\u016F. U vybran\u00FDch enzym\u016F (trypsin, lak\u00E1za, protein\u00E1za K) byly porovn\u00E1ny hodnoty %22zd\u00E1nliv\u00E9%22 Km a Vmax jejich solubiln\u00EDch a imobilizovan\u00FDch forem."@cs . "enzyme reactors; kinetic parameters; trypsin; laccase; proteinase K"@en . "Enzyme reactors represent type of biocatalyzer, which differ in essential kinetic parameters from free enzymes. Kinetic parameters are already influenced during immobilization of enzyme (choice of immobilization method, pH for binding of enzyme and type of carrier). For enzyme reactors only apparent Michaelis - Menten constant (Km,app) is possible determine. This constant Km expresses the affinity of enzyme to specific substrate including influence of microenvironment of carrier and influence of diffusion. Values of apparent Km and Vmax were determined and compared for selected free and immobilized enzymes (trypsin, laccase, proteinase K)."@en . "Horynov\u00E1, Milada" . . "8"^^ . "Enzymov\u00E9 reaktory p\u0159edstavuj\u00ED typ biokatalyz\u00E1toru, kter\u00FD se od voln\u00FDch enzym\u016F li\u0161\u00ED z\u00E1kladn\u00EDmi kinetick\u00FDmi parametry. K ovlivn\u011Bn\u00ED kinetick\u00FDch konstant doch\u00E1z\u00ED ji\u017E na \u00FArovni imobilizace enzymu, a to volbou metody imobilizace, volbou pH pro vazbu enzymu a tak\u00E9 volbou nosi\u010De. U enzymov\u00FDch reaktor\u016F lze stanovit pouze tzv. %22zd\u00E1nlivou%22 konstantu Michaelis - Mentenov\u00E9 Km (Km,app). Je to konstanta vyjad\u0159uj\u00EDc\u00ED afinitu enzymu k dan\u00E9mu substr\u00E1tu zahrnuj\u00EDc\u00ED tak\u00E9 vliv mikrookol\u00ED nosi\u010De a dif\u016Fzn\u00EDch vliv\u016F. U vybran\u00FDch enzym\u016F (trypsin, lak\u00E1za, protein\u00E1za K) byly porovn\u00E1ny hodnoty %22zd\u00E1nliv\u00E9%22 Km a Vmax jejich solubiln\u00EDch a imobilizovan\u00FDch forem." . "67-75" . "538823" . "Je\u017Eov\u00E1, Jana" . "Problems of determinations of Km for enzyme reactors"@en . "Problems of determinations of Km for enzyme reactors"@en . "Univerzita Pardubice" . "Problematika stanoven\u00ED Km u enzymov\u00FDch reaktor\u016F"@cs . . . "80-7194-817-9" . . . . . "2"^^ . "Problematika stanoven\u00ED Km u enzymov\u00FDch reaktor\u016F" . "Problematika stanoven\u00ED Km u enzymov\u00FDch reaktor\u016F" . . . "Slov\u00E1kov\u00E1, Marcela" . "4"^^ . . "Sborn\u00EDk p\u0159edn\u00E1\u0161ek - Monitorov\u00E1n\u00ED cizorod\u00FDch l\u00E1tek v \u017Eivotn\u00EDm prost\u0159ed\u00ED VII." . . . . "2005-03-23+01:00"^^ . "B\u00EDlkov\u00E1, Zuzana" . . "P(GA203/05/0241), Z(MSM0021627502)" . "RIV/00216275:25310/05:00002796!RIV08-MSM-25310___" . .