"\u010Cervenka, Libor" . "P(GA203/05/2106), Z(MSM0021627502)" . "RIV/00216275:25310/05:00002650!RIV08-MSM-25310___" . . "Pl\u00EDsn\u011B rodu Fusarium v potravin\u00E1ch a krmivech"@cs . "37-38" . . . "3"^^ . "ur\u010Deno pro seznam publikac\u00ED"@cs . . "Praha" . "Occurence of Fusarium in food and feed" . . "Czech Mycology" . "4"^^ . "Occurence of Fusarium in food and feed"@en . . . "Vyt\u0159asov\u00E1, Jarmila" . "533927" . . . . . "Olomouc" . "Bro\u017Ekov\u00E1, Iveta" . . . "2005-09-02+02:00"^^ . . . . . "Two PCR methods were evaluated for the successful identification of Fusarium species. The PCR was performed using a primer specifically targeted to Tri5 gene. The extraction technique of DNA by Cenis (1992) and the PCR procedure according to Edwards et al. (2001)) were compared with the DNA isolation and amplification using the Plant PCR Kit. The sensitivity of the latter method was determined using the reference strain of Fusarium graminearum CCM F-683 and the selectivity was examined using reference cultures of different genera of fungi naturally occurring in food and feedstuff. Fifty food and feedstuff samples were examined for the presence of trichothecenes-producing Fusarium. In total, seven Fusarium species were isolated, of which five reacted positively with the primer, resulting in the expected size amplicon of 260 pb. Macroscopic and microscopic features were observed on the pure culture of Fusarium and on fungi isolated in relation to their growth in different laboratory media. Morphologic"@en . "Pl\u00EDsn\u011B rodu Fusarium v potravin\u00E1ch a krmivech"@cs . "2"^^ . "RIV/00216275:25310/05:00002650" . "Two PCR methods were evaluated for the successful identification of Fusarium species. The PCR was performed using a primer specifically targeted to Tri5 gene. The extraction technique of DNA by Cenis (1992) and the PCR procedure according to Edwards et al. (2001)) were compared with the DNA isolation and amplification using the Plant PCR Kit. The sensitivity of the latter method was determined using the reference strain of Fusarium graminearum CCM F-683 and the selectivity was examined using reference cultures of different genera of fungi naturally occurring in food and feedstuff. Fifty food and feedstuff samples were examined for the presence of trichothecenes-producing Fusarium. In total, seven Fusarium species were isolated, of which five reacted positively with the primer, resulting in the expected size amplicon of 260 pb. Macroscopic and microscopic features were observed on the pure culture of Fusarium and on fungi isolated in relation to their growth in different laboratory media. Morphologic" . "Svobodov\u00E1, Lucie" . . "Occurence of Fusarium in food and feed" . . "1211-0981" . "25310" . "[46B173C38013]" . "\u010Cesk\u00E1 v\u011Bdeck\u00E1 spole\u010Dnost pro mykologii" . . . . . "Fusarium spp.; Fusarium graminearum; toxinogenic fungi; polymerase chain reaction; Tri5 gene; trichothecenes; mycotoxins"@en . . "Occurence of Fusarium in food and feed"@en .