"25310" . . "Epitope extraction technique is based on the specific digestion of a target protein followed by immunoaffinity isolation of a specific recognition peptide. This technique, in combination with mass spectrometry, has been efficiently used for epitope identification. The major goal of this work was to utilize newly developed enzyme and immunoaffinity magnetic reactors for the epitope extraction procedure and confirm the efficiency of this improved system for epitope screening of proteins. Alginic acid-coated magnetite microparticles with immobilized TPCK-trypsin provided high working efficiency with low non-specific adsorption, digestion time in minutes and low frequency of missed cleavages. The sensitivity and specificity of tryptic fragmentation of the beta-amyloid-peptide Abeta (1-40) as a model polypeptide was confirmed by Fourier-transform ion cyclotron resonance mass spectrometry analysis. The Sepharose reactor or immunoaffinity magnetic reactors, both with anti-amyloid-beta monoclonal antibodies,"@en . "Epitope extraction technique using a proteolytic microreactor combined with FTICR-MS as a tool for high-throughput screening of potential vaccine lead proteins" . "Extrakce epitop\u016F - n\u00E1stroj ke screeningu potencion\u00E1ln\u00EDch peptid\u016F vhodn\u00FDch pro vakc\u00EDnu, za pou\u017Eit\u00ED proteolytick\u00E9ho magnetick\u00E9ho reaktoru kombinovan\u00E9ho s FT-ICR MS."@cs . "European Journal of Mass Spectrometry" . . . . "RIV/00216275:25310/05:00002641" . "Epitope extraction technique is based on the specific digestion of a target protein followed by immunoaffinity isolation of a specific recognition peptide. This technique, in combination with mass spectrometry, has been efficiently used for epitope identification. The major goal of this work was to utilize newly developed enzyme and immunoaffinity magnetic reactors for the epitope extraction procedure and confirm the efficiency of this improved system for epitope screening of proteins. Alginic acid-coated magnetite microparticles with immobilized TPCK-trypsin provided high working efficiency with low non-specific adsorption, digestion time in minutes and low frequency of missed cleavages. The sensitivity and specificity of tryptic fragmentation of the beta-amyloid-peptide Abeta (1-40) as a model polypeptide was confirmed by Fourier-transform ion cyclotron resonance mass spectrometry analysis. The Sepharose reactor or immunoaffinity magnetic reactors, both with anti-amyloid-beta monoclonal antibodies," . "Epitope extraction technique using a proteolytic microreactor combined with FTICR-MS as a tool for high-throughput screening of potential vaccine lead proteins"@en . . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . . . "Ouzk\u00E1, \u0160\u00E1rka" . "Extrakce epitop\u016F - n\u00E1stroj ke screeningu potencion\u00E1ln\u00EDch peptid\u016F vhodn\u00FDch pro vakc\u00EDnu, za pou\u017Eit\u00ED proteolytick\u00E9ho magnetick\u00E9ho reaktoru kombinovan\u00E9ho s FT-ICR MS."@cs . . "5" . "6"^^ . "B\u00EDlkov\u00E1, Zuzana" . . "11" . . . . . "Stefanescu, R." . . "trypsin; magnetic microspheres; Ab (1-40) peptide; epitope extraction; immunosorbent; FT-ICR-MS; vaccine lead structure"@en . . . "2"^^ . . . "P(GA203/05/0241), Z(MSM0021627502)" . "Przybylski, M." . . "Epitope extraction technique using a proteolytic microreactor combined with FTICR-MS as a tool for high-throughput screening of potential vaccine lead proteins"@en . "Viovy, Jean-Louis" . "Je\u017Eov\u00E1, Jana" . "1469-0667" . "RIV/00216275:25310/05:00002641!RIV08-MSM-25310___" . "Koreck\u00E1, Lucie" . . "Extrakce epitop\u016F - n\u00E1stroj ke screeningu potencion\u00E1ln\u00EDch peptid\u016F vhodn\u00FDch pro vakc\u00EDnu, za pou\u017Eit\u00ED proteolytick\u00E9ho magnetick\u00E9ho reaktoru kombinovan\u00E9ho s FT-ICR MS."@cs . "[83F874B5AB38]" . . "8"^^ . "520448" . "Epitope extraction technique using a proteolytic microreactor combined with FTICR-MS as a tool for high-throughput screening of potential vaccine lead proteins" . "489-495" . "Cecal, R." . .