"Lebedev, Y.B." . . "Lukyanov, S." . . "RIV/00216224:14740/14:00079244!RIV15-MSM-14740___" . . "Bolotin, D. A." . "10.4049/jimmunol.1302064" . . . . "000332702700018" . . . "Chudakov, Dmitriy" . "P(ED1.1.00/02.0068)" . "Shugay, M." . . "Age-Related Decrease in TCR Repertoire Diversity Measured with Deep and Normalized Sequence Profiling" . "T-CELL REPERTOIRE; RECEPTOR DIVERSITY; THYMIC INVOLUTION; IMMUNE DEFENSE; OLD PRIMATES; IFN-GAMMA; TNF-ALPHA; RESPONSES; HOMEOSTASIS; GENERATION"@en . "10"^^ . "Bogdanova, E. A." . "Age-Related Decrease in TCR Repertoire Diversity Measured with Deep and Normalized Sequence Profiling"@en . "1"^^ . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . . . "The decrease of TCR diversity with aging has never been studied by direct methods. In this study, we combined high-throughput Illumina sequencing with unique cDNA molecular identifier technology to achieve deep and precisely normalized profiling of TCR beta repertoires in 39 healthy donors aged 6-90 y. We demonstrate that TCR beta diversity per 10(6) T cells decreases roughly linearly with age, with significant reduction already apparent by age 40. The percentage of naive T cells showed a strong correlation with measured TCR diversity and decreased linearly up to age 70. Remarkably, the oldest group (average age 82 y) was characterized by a higher percentage of naive CD4(+) T cells, lower abundance of expanded clones, and increased TCR diversity compared with the previous age group (average age 62 y), suggesting the influence of age selection and association of these three related parameters with longevity."@en . . "6" . "Chudakov, Dmitriy" . . "Age-Related Decrease in TCR Repertoire Diversity Measured with Deep and Normalized Sequence Profiling" . "12"^^ . "0022-1767" . "The decrease of TCR diversity with aging has never been studied by direct methods. In this study, we combined high-throughput Illumina sequencing with unique cDNA molecular identifier technology to achieve deep and precisely normalized profiling of TCR beta repertoires in 39 healthy donors aged 6-90 y. We demonstrate that TCR beta diversity per 10(6) T cells decreases roughly linearly with age, with significant reduction already apparent by age 40. The percentage of naive T cells showed a strong correlation with measured TCR diversity and decreased linearly up to age 70. Remarkably, the oldest group (average age 82 y) was characterized by a higher percentage of naive CD4(+) T cells, lower abundance of expanded clones, and increased TCR diversity compared with the previous age group (average age 62 y), suggesting the influence of age selection and association of these three related parameters with longevity." . . "192" . . . . . "14740" . "Journal of immunology" . "Britanova, O. V." . . "[F90E7AC9094D]" . "Staroverov, D. B." . . "Age-Related Decrease in TCR Repertoire Diversity Measured with Deep and Normalized Sequence Profiling"@en . "Merzlyak, E. M." . "RIV/00216224:14740/14:00079244" . "Mamedov, I.Z." . . "Putintseva, E. V." . "Turchaninova, M. A." . "1851" .