. "000338271200016" . "Disparate HDV ribozyme crystal structures represent intermediates on a rugged free-energy landscape"@en . "HEPATITIS-DELTA-VIRUS; MOLECULAR-DYNAMICS SIMULATIONS; SELF-CLEAVING RIBOZYMES; ACID-BASE CATALYSIS; MULTICHANNEL REACTION-MECHANISM; LOCAL CONFORMATIONAL-CHANGES; DIVALENT METAL-IONS; GENERAL ACID; HAIRPIN RIBOZYME; GENOMIC RIBOZYME"@en . . "Sripathi, Kamali N." . . "Tay, Wendy W." . "Ban\u00E1\u0161, Pavel" . . "Disparate HDV ribozyme crystal structures represent intermediates on a rugged free-energy landscape"@en . . "10.1261/rna.044982.114" . "[B934F6035378]" . "20" . . "11743" . "17"^^ . . "Disparate HDV ribozyme crystal structures represent intermediates on a rugged free-energy landscape" . "RIV/00216224:14740/14:00076675!RIV15-MSM-14740___" . . "http://rnajournal.cshlp.org/content/20/7/1112.long" . . . "14740" . "Otyepka, Michal" . . "Walter, Nils G." . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "7" . "6"^^ . "1355-8382" . "I, P(ED1.1.00/02.0068), P(ED2.1.00/03.0058), P(EE2.3.20.0017), P(EE2.3.20.0058), P(GAP208/12/1878)" . "Disparate HDV ribozyme crystal structures represent intermediates on a rugged free-energy landscape" . . . . . . . . . . "1"^^ . "\u0160poner, Ji\u0159\u00ED" . . . "The hepatitis delta virus (HDV) ribozyme is a member of the class of small, self-cleaving catalytic RNAs found in a wide range of genomes from HDV to human. Both pre- and post-catalysis (precursor and product) crystal structures of the cis-acting genomic HDV ribozyme have been determined. These structures, together with extensive solution probing, have suggested that a significant conformational change accompanies catalysis. A recent crystal structure of a trans-acting precursor, obtained at low pH and by molecular replacement from the previous product conformation, conforms to the product, raising the possibility that it represents an activated conformer past the conformational change. Here, using fluorescence resonance energy transfer (FRET), we discovered that cleavage of this ribozyme at physiological pH is accompanied by a structural lengthening in magnitude comparable to previous trans-acting HDV ribozymes." . . . . "RNA" . "The hepatitis delta virus (HDV) ribozyme is a member of the class of small, self-cleaving catalytic RNAs found in a wide range of genomes from HDV to human. Both pre- and post-catalysis (precursor and product) crystal structures of the cis-acting genomic HDV ribozyme have been determined. These structures, together with extensive solution probing, have suggested that a significant conformational change accompanies catalysis. A recent crystal structure of a trans-acting precursor, obtained at low pH and by molecular replacement from the previous product conformation, conforms to the product, raising the possibility that it represents an activated conformer past the conformational change. Here, using fluorescence resonance energy transfer (FRET), we discovered that cleavage of this ribozyme at physiological pH is accompanied by a structural lengthening in magnitude comparable to previous trans-acting HDV ribozymes."@en . . "RIV/00216224:14740/14:00076675" . .