"http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0109875" . . "recombinant antibodies; immunomodulation; inhibition; protein-protein interactions; cytokinin signaling pathway"@en . . . . . . . "9"^^ . "10.1371/journal.pone.0109875" . "49313" . "Hrdinov\u00E1, Vendula" . . "Hej\u00E1tko, Jan" . "RIV/00216224:14740/14:00074131" . "6"^^ . . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . . "10" . "PLOS ONE : Public Library of Science" . "M\u00FCller, Bruno" . "Targeted In Vivo Inhibition of Specific Protein\u2013Protein Interactions Using Recombinant Antibodies"@en . . . . . . "Targeted In Vivo Inhibition of Specific Protein\u2013Protein Interactions Using Recombinant Antibodies" . "P(ED1.1.00/02.0068), P(EE2.3.20.0189), P(GA13-25280S), P(GA521/09/1699), P(GAP305/11/0756)" . "000343941200081" . "9" . "1932-6203" . . "[0ADE81E74929]" . . . . "RIV/00216224:14740/14:00074131!RIV15-MSM-14740___" . . "Janda, Lubom\u00EDr" . "Targeted In Vivo Inhibition of Specific Protein\u2013Protein Interactions Using Recombinant Antibodies" . . "Targeted In Vivo Inhibition of Specific Protein\u2013Protein Interactions Using Recombinant Antibodies"@en . "With the growing availability of genomic sequence information, there is an increasing need for gene function analysis. Antibody-mediated \u201Csilencing\u201D represents an intriguing alternative for the precise inhibition of a particular function of biomolecules. Here, we describe a method for selecting recombinant antibodies with a specific purpose in mind, which is to inhibit intrinsic protein\u2013protein interactions in the cytosol of plant cells. Experimental procedures were designed for conveniently evaluating desired properties of recombinant antibodies in consecutive steps. Our selection method was successfully used to develop a recombinant antibody inhibiting the interaction of ARABIDOPSIS HISTIDINE PHOSPHOTRANSFER PROTEIN 3 with such of its upstream interaction partners as the receiver domain of CYTOKININ INDEPENDENT HISTIDINE KINASE 1. The specific down-regulation of the cytokinin signaling pathway in vivo demonstrates the validity of our approach."@en . "Conrad, Udo" . "14740" . "Z\u00E1brady, Matej" . . . . "4"^^ . "With the growing availability of genomic sequence information, there is an increasing need for gene function analysis. Antibody-mediated \u201Csilencing\u201D represents an intriguing alternative for the precise inhibition of a particular function of biomolecules. Here, we describe a method for selecting recombinant antibodies with a specific purpose in mind, which is to inhibit intrinsic protein\u2013protein interactions in the cytosol of plant cells. Experimental procedures were designed for conveniently evaluating desired properties of recombinant antibodies in consecutive steps. Our selection method was successfully used to develop a recombinant antibody inhibiting the interaction of ARABIDOPSIS HISTIDINE PHOSPHOTRANSFER PROTEIN 3 with such of its upstream interaction partners as the receiver domain of CYTOKININ INDEPENDENT HISTIDINE KINASE 1. The specific down-regulation of the cytokinin signaling pathway in vivo demonstrates the validity of our approach." .