. . "10.1007/s13277-012-0573-2" . . "RIV/00216224:14740/13:00065569" . "Poprach, Alexandr" . "MiR-210 expression in tumor tissue and in vitro effects of its silencing in renal cell carcinoma"@en . "MiR-210 expression in tumor tissue and in vitro effects of its silencing in renal cell carcinoma" . "Vyzula, Rostislav" . . . "Iliev, Robert" . "RIV/00216224:14740/13:00065569!RIV14-MZ0-14740___" . "34" . . . "000313875400056" . . . . "1" . . "88520" . . . "MiR-210 expression in tumor tissue and in vitro effects of its silencing in renal cell carcinoma"@en . "MiR-210 expression in tumor tissue and in vitro effects of its silencing in renal cell carcinoma" . "Lakom\u00FD, Radek" . "Nekvindov\u00E1, Jana" . . "1010-4283" . . "14740" . "11"^^ . "Renal cell carcinoma; MicroRNA; MiR-210; ACHN; CAKI-2; Proliferation; Invasiveness"@en . "Radov\u00E1, Lenka" . "Slab\u00FD, Ond\u0159ej" . . . "NL - Nizozemsko" . "Tumor Biology" . . . . "4"^^ . "I, P(ED1.1.00/02.0068), P(LM2010004), P(NT13547)" . "Svoboda, Marek" . . "Renal cell carcinoma (RCC) is the most common neoplasm of adult kidney accounting for about 3 % of adult malignancies. MicroRNAs (miRNAs) are a class of naturally occurring, short non-coding RNAs that regulate gene expression at the post-transcriptional level. We determined global miRNA expression profiles of RCC and parallel renal parenchyma tissues by using quantitative reverse transcriptase-polymerase chain reaction-based TaqMan low-density arrays. Afterward, we validated the difference in miR-210 expression levels on the larger group of RCC patients (35 RCC versus 10 non-tumorous parenchyma samples). Functional in vitro experiments were performed on ACHN and CAKI-2 RCC cell lines transfected with miRNA-210 inhibitor. Cell viability, apoptosis, cell cycle, scratch wound migration assay, and invasion assay (xCELLigence) were performed. We have identified original ccRCC-specific miRNA signature in clinical samples (73 miRNAs were significantly downregulated and five miRNAs upregulated."@en . "Dole\u017Eel, Jan" . "Lojov\u00E1, Martina" . "11"^^ . "[2BC3E608D41F]" . "Be\u0161\u0161e, Andrej" . . . . . . "Renal cell carcinoma (RCC) is the most common neoplasm of adult kidney accounting for about 3 % of adult malignancies. MicroRNAs (miRNAs) are a class of naturally occurring, short non-coding RNAs that regulate gene expression at the post-transcriptional level. We determined global miRNA expression profiles of RCC and parallel renal parenchyma tissues by using quantitative reverse transcriptase-polymerase chain reaction-based TaqMan low-density arrays. Afterward, we validated the difference in miR-210 expression levels on the larger group of RCC patients (35 RCC versus 10 non-tumorous parenchyma samples). Functional in vitro experiments were performed on ACHN and CAKI-2 RCC cell lines transfected with miRNA-210 inhibitor. Cell viability, apoptosis, cell cycle, scratch wound migration assay, and invasion assay (xCELLigence) were performed. We have identified original ccRCC-specific miRNA signature in clinical samples (73 miRNAs were significantly downregulated and five miRNAs upregulated." .