"RIV/00216224:14740/12:00080243!RIV15-MSM-14740___" . . "Tur\u00E1nek, Jaroslav" . . . "3"^^ . . . . . "2"^^ . "The cell adhesion and cytotoxicity effects of the derivate of vitamin E compared for two cell lines using a piezoelectric biosensor" . "5"^^ . . "The cell adhesion and cytotoxicity effects of the derivate of vitamin E compared for two cell lines using a piezoelectric biosensor" . . "RIV/00216224:14740/12:00080243" . "The cell adhesion and cytotoxicity effects of the derivate of vitamin E compared for two cell lines using a piezoelectric biosensor"@en . "0925-4005" . . . "http://ac.els-cdn.com/S0925400512008611/1-s2.0-S0925400512008611-main.pdf?_tid=aecf51c4-ff94-11e4-83b5-00000aab0f6b&acdnat=1432197658_6fb3ce713a0c252bffd0a36ed1c9ba50" . "Skl\u00E1dal, Petr" . "The cell adhesion and cytotoxicity effects of the derivate of vitamin E compared for two cell lines using a piezoelectric biosensor"@en . "126218" . "The quartz crystal microbalance (QCM) was used to construct a piezoelectric biosensor utilizing rat liver epithelial cells (WB F344) and lung melanoma cells (B16F10) as biorecognition elements. The cells adhered on the gold surface modified by either hydrophobic polystyrene or fibronectin. The simultaneous monitoring of changes in the resonant frequency Delta f and the resistance Delta R corresponded with the mass and viscoelastic properties of the adhered cells. In addition, the bound cells were stained using fluorescent probes to evaluate their viability on the non-transparent gold surface. Furthermore, this piezoelectric biosensor was used for monitoring of drug-induced apoptosis initiated by micromolar concentrations of alpha-tocopherol amidomalate (alpha-TAM), a potential anticancer drug. The characteristic features of apoptosis as shrinkage and disintegration of cell-cell contacts could be monitored by QCM." . "November" . . "10.1016/j.snb.2012.08.047" . "Fohlerov\u00E1, Zdenka" . "P(ED1.1.00/02.0068)" . "Quartz crystal microbalance; Fluorescence microscopy; Cell adhesion; Extracellular matrix; Apoptosis; Vitamin E"@en . "The quartz crystal microbalance (QCM) was used to construct a piezoelectric biosensor utilizing rat liver epithelial cells (WB F344) and lung melanoma cells (B16F10) as biorecognition elements. The cells adhered on the gold surface modified by either hydrophobic polystyrene or fibronectin. The simultaneous monitoring of changes in the resonant frequency Delta f and the resistance Delta R corresponded with the mass and viscoelastic properties of the adhered cells. In addition, the bound cells were stained using fluorescent probes to evaluate their viability on the non-transparent gold surface. Furthermore, this piezoelectric biosensor was used for monitoring of drug-induced apoptosis initiated by micromolar concentrations of alpha-tocopherol amidomalate (alpha-TAM), a potential anticancer drug. The characteristic features of apoptosis as shrinkage and disintegration of cell-cell contacts could be monitored by QCM."@en . . "[96F4C8C40D2D]" . "000311329000022" . . "CH - \u0160v\u00FDcarsk\u00E1 konfederace" . . . "174" . . "Sensors and Actuators B Chemical" . . "14740" . .