. . "Hrazdilov\u00E1, Krist\u00FDna" . "Air2p is critical for the assembly and RNA-binding of the TRAMP complex and the KOW domain of Mtr4p is crucial for exosome activation"@en . "Nucleic Acids Research" . "P(ED1.1.00/02.0068), P(GAP305/10/1490), P(GAP305/11/1095), P(GBP305/12/G034)" . . "RNA recognition; binding; RNA processing; RNA-protein interaction"@en . . "RIV/00216224:14740/12:00057391!RIV13-GA0-14740___" . "Sa\u0148udo Arce, Maria" . "RIV/00216224:14740/12:00057391" . . "Air2p is critical for the assembly and RNA-binding of the TRAMP complex and the KOW domain of Mtr4p is crucial for exosome activation" . "http://nar.oxfordjournals.org/content/early/2012/03/08/nar.gks223.abstract" . . . . "10.1093/nar/gks223" . . . "14740" . "12" . "S\u00E1razov\u00E1, Marie" . . . "000305829000049" . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . "Trf4/5p-Air1/2p-Mtr4p polyadenylation complex (TRAMP) is an essential component of nuclear RNA surveillance in yeast. It recognizes a variety of nuclear transcripts produced by all three RNA polymerases, adds short poly(A) tails to aberrant or unstable RNAs and activates the exosome for their degradation. Despite the advances in understanding the structural features of the isolated complex subunits or their fragments, the details of complex assembly, RNA recognition and exosome activation remain poorly understood. Here we provide the first understanding of the RNA binding mode of the complex. We show that Air2p is an RNA-binding subunit of TRAMP. We identify the zinc knuckles (ZnK) 2, 3 and 4 as the RNA-binding domains, and reveal the essentiality of ZnK4 for TRAMP4 polyadenylation activity. Furthermore, we identify Air2p as the key component of TRAMP4 assembly providing bridging between Mtr4p and Trf4p."@en . . "[FC9D1B611D28]" . "10"^^ . "Va\u0148\u00E1\u010Dov\u00E1, \u0160t\u011Bp\u00E1nka" . "\u010Cern\u00E1, Hana" . "Pasulka, Josef" . "121406" . "0305-1048" . "40" . . . . "15"^^ . . "Trf4/5p-Air1/2p-Mtr4p polyadenylation complex (TRAMP) is an essential component of nuclear RNA surveillance in yeast. It recognizes a variety of nuclear transcripts produced by all three RNA polymerases, adds short poly(A) tails to aberrant or unstable RNAs and activates the exosome for their degradation. Despite the advances in understanding the structural features of the isolated complex subunits or their fragments, the details of complex assembly, RNA recognition and exosome activation remain poorly understood. Here we provide the first understanding of the RNA binding mode of the complex. We show that Air2p is an RNA-binding subunit of TRAMP. We identify the zinc knuckles (ZnK) 2, 3 and 4 as the RNA-binding domains, and reveal the essentiality of ZnK4 for TRAMP4 polyadenylation activity. Furthermore, we identify Air2p as the key component of TRAMP4 assembly providing bridging between Mtr4p and Trf4p." . "H\u00F3bor, Fruzsina" . "Lal\u00E1kov\u00E1, Jana" . "Holub, Peter" . "10"^^ . . . . "\u0160tefl, Richard" . . "Air2p is critical for the assembly and RNA-binding of the TRAMP complex and the KOW domain of Mtr4p is crucial for exosome activation"@en . . . . "Sa\u0148udo Arce, Maria" . . . "Air2p is critical for the assembly and RNA-binding of the TRAMP complex and the KOW domain of Mtr4p is crucial for exosome activation" . "H\u00F3bor, Fruzsina" . .