"P(GAP207/10/0321), P(GPP207/11/P185), Z(MSM0021622413)" . "14740" . "Wimmerov\u00E1, Michaela" . . "rotein-carbohydrate interactions are usually characterized by a low affinity for monovalent ligands that is balanced by multivalency resulting in high avidity for ligands with several potential epitops. However, recent characterization of lectins involved in pathogenesis has demonstrated their much higher affinity even towards monosaccharide than that observed for plant or animal lectins [1]. This feature allows to use such lectins as a more/less specific tool, for example, for evaluation of protein glycosylation or isolation of glyco-conjugates. Random mutagenesis is often used in protein engineering for producing proteins with altered or improved properties. In our work several methodical directions have been applied. Error-prone PCR is based on error-prone DNA polymerase which introduces nucleotide changes during extension of amplified DNA."@en . "Protein engineering of lectins - in silico and in vitro approaches" . "lectin; error prone PCR; random mutagenesis"@en . "5"^^ . "rotein-carbohydrate interactions are usually characterized by a low affinity for monovalent ligands that is balanced by multivalency resulting in high avidity for ligands with several potential epitops. However, recent characterization of lectins involved in pathogenesis has demonstrated their much higher affinity even towards monosaccharide than that observed for plant or animal lectins [1]. This feature allows to use such lectins as a more/less specific tool, for example, for evaluation of protein glycosylation or isolation of glyco-conjugates. Random mutagenesis is often used in protein engineering for producing proteins with altered or improved properties. In our work several methodical directions have been applied. Error-prone PCR is based on error-prone DNA polymerase which introduces nucleotide changes during extension of amplified DNA." . "Adam, Jan" . . . "Mr\u00E1zkov\u00E1, Jana" . . . . "[B68CC778CCD3]" . "RIV/00216224:14740/11:00050180!RIV12-GA0-14740___" . . "5"^^ . "Protein engineering of lectins - in silico and in vitro approaches"@en . "Protein engineering of lectins - in silico and in vitro approaches"@en . . . . . . . "Ko\u010Da, Jaroslav" . "224813" . . "RIV/00216224:14740/11:00050180" . . "Protein engineering of lectins - in silico and in vitro approaches" . . "Pokorn\u00E1, Martina" . . . . . . . .