. "14740" . "6"^^ . . "[AC4A187593FC]" . "CE-LIF of Green Fluorescent Fusion Proteins for Investigation of Phosphorylation" . . . "RIV/00216224:14740/11:00050124!RIV12-GA0-14740___" . "P(ED1.1.00/02.0068), P(GA203/09/1025), Z(MSM0021622415)" . . . . . "Ryvolov\u00E1, Mark\u00E9ta" . . "CE-LIF; phosphorylation; AHP proteins"@en . "RIV/00216224:14740/11:00050124" . . "Preisler, Jan" . "189364" . . . "V\u00E1lkov\u00E1, Martina" . . . "CE-LIF of Green Fluorescent Fusion Proteins for Investigation of Phosphorylation" . . . "Hej\u00E1tko, Jan" . "Michalcov\u00E1, Lenka" . . . . "In this study, CE-LIF with 405 nm laser diode was used to study AHP5 fused with GFP. AHP5-GFP fusion proteins overexpressed in E. coli were used for initial optimization of CE-LIF technique because of lower concentrations of the fusion protein produced by plant. The stability of the AHP5-GFP sample was examined; the complex was found to be unstable with GFP being released. The GFP release was not affected by either a long-term storage at -80 and -20 C or the addition of one of the inhibitors (EDTA, PMSF). These results were confirmed by the western blotting. The phosphorylation of the sample AHP5-GFP in vitro is under investigation. Gel filtration was used to obtain mass fractions for better understanding electrophoregrams. The mass fractions were investigated by CE-LIF and Western blotting-LIF and Western blotting." . "CE-LIF of Green Fluorescent Fusion Proteins for Investigation of Phosphorylation"@en . . "In this study, CE-LIF with 405 nm laser diode was used to study AHP5 fused with GFP. AHP5-GFP fusion proteins overexpressed in E. coli were used for initial optimization of CE-LIF technique because of lower concentrations of the fusion protein produced by plant. The stability of the AHP5-GFP sample was examined; the complex was found to be unstable with GFP being released. The GFP release was not affected by either a long-term storage at -80 and -20 C or the addition of one of the inhibitors (EDTA, PMSF). These results were confirmed by the western blotting. The phosphorylation of the sample AHP5-GFP in vitro is under investigation. Gel filtration was used to obtain mass fractions for better understanding electrophoregrams. The mass fractions were investigated by CE-LIF and Western blotting-LIF and Western blotting."@en . . . "CE-LIF of Green Fluorescent Fusion Proteins for Investigation of Phosphorylation"@en . . . "6"^^ . "Dopitov\u00E1, Radka" .