"Crystallization and structure determination of delta-subunit of RNApolymerase" . . . . "[E187533C253D]" . "Wimmerov\u00E1, Michaela" . . "delta-subunit RNA polymerase crystallization structure"@en . "4"^^ . "\u0160anderov\u00E1, Hana" . "RIV/00216224:14740/11:00049774!RIV12-GA0-14740___" . . "Demo, Gabriel" . "The structure of N-terminal domain was determined by NMR. It consists mainly of three alpha-helixes and one short beta-sheet, yet the N-terminal part remains unstructured. The cause of this flexibility is probably the His6-Tag attached at the N-terminus.The N-terminal domain of delta subunit was conquested to high-throughput screening (sitting drop), where several crystallization conditions were found.Higher quality crystals were obtained after 7-9 days with an average size 150 x 100 micrometers.Diffraction data were collected at ESRF Grenoble, ID-23. The resolution of the structure was 3.5 Angstrom. The data were processed by MOSFLM and the determination of the structure was done by molecular replacement with NMR structure used as a model."@en . "Crystallization and structure determination of delta-subunit of RNApolymerase" . "\u017D\u00EDdek, Luk\u00E1\u0161" . . . "14740" . . "Crystallization and structure determination of delta-subunit of RNApolymerase"@en . . . . . "5"^^ . . . . . "192371" . "Crystallization and structure determination of delta-subunit of RNApolymerase"@en . "RIV/00216224:14740/11:00049774" . . . . "P(GA204/09/0583), P(GD301/09/H004), Z(MSM0021622413)" . "The structure of N-terminal domain was determined by NMR. It consists mainly of three alpha-helixes and one short beta-sheet, yet the N-terminal part remains unstructured. The cause of this flexibility is probably the His6-Tag attached at the N-terminus.The N-terminal domain of delta subunit was conquested to high-throughput screening (sitting drop), where several crystallization conditions were found.Higher quality crystals were obtained after 7-9 days with an average size 150 x 100 micrometers.Diffraction data were collected at ESRF Grenoble, ID-23. The resolution of the structure was 3.5 Angstrom. The data were processed by MOSFLM and the determination of the structure was done by molecular replacement with NMR structure used as a model." . . "Papou\u0161kov\u00E1, Veronika" .