"36" . "1"^^ . "RIV/00216224:14330/99:00003925" . "RIV/00216224:14330/99:00003925!RIV/2002/GA0/143302/N" . "Holub\u00E1\u0159ov\u00E1, Alena" . . "757666" . . "Vliv cytoskelet\u00E1ln\u00EDch inhibitor\u016F na meiosu a sporulaci u d\u011Bl\u00EDc\u00EDch se kvasinek" . . . "P(GA204/97/1150)" . "Homothalick\u00E9 kvasinky Schizosaccharomyces japonicus var. versatilis kultivovan\u00E9 v m\u00E9diu se sladinov\u00FDm extraktem a gluk\u00F3zou, po dosa\u017Een\u00ED stacion\u00E1rn\u00ED f\u00E1ze r\u016Fstu za\u010D\u00EDnaj\u00ED aglutinovat, nast\u00E1v\u00E1 p\u00E1rov\u00E1n\u00ED, meiosa a sporulace (1). V r\u016Fzn\u00FDch f\u00E1z\u00EDch r\u016Fstu byly ke kultu\u0159e p\u0159id\u00E1ny inhibitory mikrotubul\u016F a mikrofilament. Mikrotubul\u00E1rn\u00ED inhibitory benomyl (BEM), thiabendazol (TBZ) a methylbenzimidazol (MBC) byly aplikov\u00E1ny v koncentrac\u00EDch 10 - 100 mg/ml v r\u016Fzn\u00FDch f\u00E1z\u00EDch v\u00FDvoje kultury a byl sledov\u00E1n jejich cytologick\u00FD efekt. MBC inhiboval r\u016Fst kultury a\u017E v koncentrac\u00EDch 150 mg/ml a indukoval cytol\u00FDzu bun\u011Bk. BEM dezintegroval mikrotubuly spolehliv\u011B p\u0159i 30 mg/ml, av\u0161ak i ni\u017E\u0161\u00ED koncentrace inhibitoru indukovaly poruchy v po\u010Dtu spor: m\u00EDsto typick\u00FDch 8 spor se v asc\u00EDch vyskytoval ni\u017E\u0161\u00ED po\u010Det r\u016Fzn\u011B velk\u00FDch spor, \u010Dasto byla sporulace abortivn\u00ED. TBZ inhiboval r\u016Fst kultury ji\u017E od 30 mg/ml, kdy u n\u011Bkter\u00FDch bun\u011Bk byly mikrotubuly dezintegrov\u00E1ny \u010D\u00E1ste\u010Dn\u011B, p\u0159i 50 mg/ml vymizely mikrotubuly zcela. I v koncentrac\u00EDch 30 mg/ml" . "Brno" . "Vliv cytoskelet\u00E1ln\u00EDch inhibitor\u016F na meiosu a sporulaci u d\u011Bl\u00EDc\u00EDch se kvasinek"@cs . "Brno" . . . . "Homothallic yeasts Schizosaccharomyces japonicus var. versatilis, undergo mass mating, meiosis and sporulation when they reach stationary phase after growth in malt extract broth glucose medium. Microtubular inhibitors benomyl (BEM), thiabendazole (TBZ) and methylbenzimidazole (MBC) were applied in concentrations 10 -100 mikro g/ml at different phases of culture growth and their cytological effects were followed microscopically. MBC inhibited the increase of cell number at 150 mikro g/ml and induced cytolysis. BEM disintegrated microtubules completely at 30 mikro g/ml,lower concentrations of inhibitor disturbed sporulation asci with 1 to 7 spore appeared instead of regular 8 spores per ascus. Frequently the sporulation process turned out abortive. TBZ affected the growth curve from concentration 30 mikro g/ml, where part of the cell population showed the microtubules partly disrupted. The microtubules disappeared completely at 50 mikro g/ml. Mating projections were formed at 30 or even at 50 mikro g/"@en . "[765DA20F7E7A]" . . . "Effect of cytoskeletal inhibitors of meiosis and sporulation of fisssion yeast"@en . "Vliv cytoskelet\u00E1ln\u00EDch inhibitor\u016F na meiosu a sporulaci u d\u011Bl\u00EDc\u00EDch se kvasinek"@cs . . . . . "Svoboda, Augustin" . "0"^^ . "Effect of cytoskeletal inhibitors of meiosis and sporulation of fisssion yeast"@en . "0"^^ . "2"^^ . "\u010Cs. biologick\u00E1 spole\u010Dnost" . "2"^^ . "Effect of cytoskeletal inhibitors of meiosis and sporulation of fisssion yeast"@en . . "VII. Cytoskelet\u00E1ln\u00ED klub" . "14330" . . "1999-01-01+01:00"^^ . "Vliv cytoskelet\u00E1ln\u00EDch inhibitor\u016F na meiosu a sporulaci u d\u011Bl\u00EDc\u00EDch se kvasinek" .