. . . "Recent findings suggest that apoptotic protein apoptosis-inducing factor (AIF) may also play an important non-apoptotic function inside mitochondria. AIF was proposed to be an important component of respiratory chain complex I that is the major producer of superoxide radical. The possible role of AIF is still controversial. Superoxide production could be used as a valuable measure of complex I function, because the majority of superoxide is produced there. Therefore, we employed superoxide-specific mitochondrial fluorescence dye for detection of superoxide production. We studied an impact of AIF knockdown on function of mitochondrial complex I by analyzing superoxide production in selected cell lines." . . "Knockdown of apoptosis-inducing factor disrupts function of respiratory complex I"@en . "3" . "RIV/00216224:14330/12:00057522!RIV13-GA0-14330___" . "superoxide; fluorescence microscopy; image analysis; mitochondria; telomerase"@en . "P(GBP302/12/G157)" . "BIOCELL" . "Va\u0159echa, Miroslav" . "Matula, Pavel" . "5"^^ . "P\u00E1clov\u00E1, Daniela" . "Proch\u00E1zkov\u00E1, Ji\u0159ina" . "6"^^ . . "6"^^ . "Kozubek, Michal" . . . . . "36" . . "AR - Argentinsk\u00E1 republika" . . "Knockdown of apoptosis-inducing factor disrupts function of respiratory complex I" . . "Cmarko, Du\u0161an" . "14330" . "Recent findings suggest that apoptotic protein apoptosis-inducing factor (AIF) may also play an important non-apoptotic function inside mitochondria. AIF was proposed to be an important component of respiratory chain complex I that is the major producer of superoxide radical. The possible role of AIF is still controversial. Superoxide production could be used as a valuable measure of complex I function, because the majority of superoxide is produced there. Therefore, we employed superoxide-specific mitochondrial fluorescence dye for detection of superoxide production. We studied an impact of AIF knockdown on function of mitochondrial complex I by analyzing superoxide production in selected cell lines."@en . "0327-9545" . . . "144834" . . . "Knockdown of apoptosis-inducing factor disrupts function of respiratory complex I"@en . . . . . . . "[9D46A46D507B]" . "RIV/00216224:14330/12:00057522" . "Knockdown of apoptosis-inducing factor disrupts function of respiratory complex I" .