. . "Krontor\u00E1d, Petr" . "9"^^ . . "Neoplasma" . "microarray; cell profiling; protein expression; mRNA expression; HL-60"@en . . . "Kozubek, Michal" . "RIV/00216224:14330/09:00034277!RIV10-MSM-14330___" . "RIV/00216224:14330/09:00034277" . . "14330" . "Peterkov\u00E1, Martina" . "This study combines mRNA and protein analysis using cDNA and antibody microarray techniques, respectively. These create a novel, integrated perspective into cellular molecular profiles. The aims of this study were to establish a reliable way of integrating these two approaches in order to obtain complex molecular profiles of the cell and to find suitable methods to normalize the data obtained using these approaches. Antibody microarray and cDNA microarray techniques were used to study expression alterations in HL-60 cells that were differentiated into granulocytes using all-trans retinoic acid (ATRA). We selected this model to evaluate this combined profiling technique because the expression levels of most of the mRNA and protein species in these cells are not altered; therefore it is easier to track and define those species that are changed. The proteins whose levels were altered included cmyc, c-jun, Pyk2, FAK, PKC, TRF1, NF-kappaB and certain caspase types."@en . . "CZ - \u010Cesk\u00E1 republika" . . . . . "56" . "Combination of mRNA and protein microarray analysis in complex cell profiling" . "Stejskal, Stanislav" . "Krontor\u00E1d Koutn\u00E1, Irena" . . . "Combination of mRNA and protein microarray analysis in complex cell profiling" . . "Combination of mRNA and protein microarray analysis in complex cell profiling"@en . "Ru\u010Dka, Zden\u011Bk" . "Combination of mRNA and protein microarray analysis in complex cell profiling"@en . "This study combines mRNA and protein analysis using cDNA and antibody microarray techniques, respectively. These create a novel, integrated perspective into cellular molecular profiles. The aims of this study were to establish a reliable way of integrating these two approaches in order to obtain complex molecular profiles of the cell and to find suitable methods to normalize the data obtained using these approaches. Antibody microarray and cDNA microarray techniques were used to study expression alterations in HL-60 cells that were differentiated into granulocytes using all-trans retinoic acid (ATRA). We selected this model to evaluate this combined profiling technique because the expression levels of most of the mRNA and protein species in these cells are not altered; therefore it is easier to track and define those species that are changed. The proteins whose levels were altered included cmyc, c-jun, Pyk2, FAK, PKC, TRF1, NF-kappaB and certain caspase types." . . . . . "P(2B06052), Z(MSM0021622430)" . "7"^^ . . . "\u0160imara, Pavel" . . "7"^^ . . . "[9E51CE8BA297]" . "2/2009" . . . "0028-2685" . "307527" .